Services currently offered by the Analytical Pharmacology Core facility:
- Initial consultation on research projects
- Study design and protocol review
- Development, implementation, and validation of analytical methods.
- Sample analysis (LC/MS/MS, GC/MS, HPLC, and AAS).
- Pharmacokinetic programming and data analysis
- Sample acquisition, tracking, and storage.
- Sample acquisition, storage analysis, including HPLC, LC/MS/MS and GC/MS, and microbiological assay methodologies
- Participation in collaborative writing
Analytical capabilities cover a wide range of available methods including:
- LC/MC/MS (Waters Quattro Ultima and Quattro Premier XE
- GC/MS (2 Shimadzu QP-5000's
- HPLC with UV/Vis, fluorescence, and electrochemical detection (Shimadzu and Thermo Separations)
- AAS with graphite furnace (PerkinElmer AAnalyst 300
HPLC capability includes four complete systems, which are integrated directly to dedicated computers running EZChrom software for automated post-run analysis. Three of the HPLC systems include automated injection systems for more convenient analysis of a large number of samples.
Instrument control and data acquisition for the Micromass mass detector and the associated Agilent HPLC are coordinated through a MassLynx-NT workstation running MassLynx and QuanLynx software. The GC/MS instrumentation is composed of a Shimadzu Model QP-5000 EI gas chromatograph/mass spectrometer, interfaced directly to a dedicated computer running CLASS-5000 software.
In addition to the chromatographic instrumentation, a Perkin Elmer AAnalyst 300 flameless atomic absorption spectrometer (AA) is available for the determination of metals and metal-containing compounds such as cisplatin and its analogs. Sample analysis by AA utilizes an automated injection system, with data acquisition and analysis using WinLab software on a dedicated computer.