HiSeq 2000, Illumina
Yield: 240 GB per flow cell
Read lengths: 2 x 100 bases
The HiSeq 2000 sequencing system offers unprecedented output and a breakthrough user experience. Leveraging Illumina's proven and widely-adopted, reversible terminator-based sequencing by synthesis chemistry in combination with innovative engineering, HiSeq 2000 delivers the industry's highest sequencing output and fastest data generation rate.
454 GS-FLX, Roche
Yield: 600 MB
Read lengths: up to 400 bases
The 454 Genome Sequencer FLX Titanium platform generates about 1,000,000 reads with an average length of 300-500bp in a typical run. It is an excellent platform for de novo sequencing and for projects that require longer read lengths. The GS FLX Titanium accommodates shotgun, paired-end and amplicon sequencing runs.
The Genome Analyzer is capable of a wide array of applications: if adapters can be added to it, it can be sequenced. Below is a list of common applications.
Genome Sequencing (gDNA)
Leverage single- or paired-end reads for:
- Single Nucleotide Variations from whole-genome and targeted resequencing
- Copy Number Variations and chromosomal rearrangements
- De novo sequencing and assembly
Targeted Exome Capture (Seq-cap)
The exome represents all the exons in the human genome (i.e., the transcribed region of the genome). Exome sequencing is an efficient strategy to selectively sequence the coding regions of the human genome to identify novel genes associated with rare and common disorders.
Whole Transcriptome Sequencing (mRNA-seq)
With RNA sequencing, you can characterize all transcriptional activity, coding and noncoding in any organism without a priori assumptions. Illumina's unique combination of long and short reads, single and paired-end sequencing, strand specificity, and capacity for tens of millions to billions of reads per run allows you to:
- Annotate coding SNPs
- Discover transcript isoforms
- Identify regulatory RNAs
- Characterize splice junctions
- Determine the relative abundance of transcripts
DNA-Protein Interactions (ChIP-seq)
Generate genome-wide profiles of DNA-protein interactions. Identify DNA fragments bound to:
- Transcription factors
Small RNA Discovery and Analysis (smRNA-seq)
Discover and profile all forms of small noncoding RNA. No prior sequence or structure information is required.
- Novel miRNAs
- Differential expression
- Sequence variants
Discover methylation variable positions (MVPs) across the whole genome by sequencing DNA from:
- Methylation-sensitive restriction digests
- Bisulfite conversion
- Anti-methyl C precipitation
NanoDrop® ND-1000 UV-Vis
The NanoDrop® ND-1000 UV-Vis Spectrophotometer enables accurate analyses of as low as 1 ml of sample, with good reproducibility.
Agilent 2100 Bioanalyzer
The Agilent 2100 Bioanalyzer is a microfluidics-based platform that offers solutions for the analysis of DNA, RNA, proteins and cells.