The High Throughput Screening (HTS) Core facility offers the following services:
Discussing with investigators the various approaches in identifying/screening compounds
Assisting investigators in developing new assays or adapting their low throughput assays into automated, high throughput screening formats. Assays may involve proteins, cell lines, or simple model organisms.
The core subjects the assays to high throughput screening and analyzes the results to identify active hits for each assay.
Performing structure-activity relationship (SAR) analysis of compounds demonstrating activity in the individual assays.
The core assists in collecting, maintaining and interpreting data for project investigators.
Drug discovery is a multi-disciplinary effort. In order to advance a project efficiently, it is important that everyone involved be up-to-date with the progress of the project such as the latest hits/leads. It is also crucial to form a consensus about the characteristics of the compounds, e.g. potency, selectivity, etc., so that the team can effectively decide whether to go further with a particular compound and/or its derivatives. The HTS Core services provide an effective framework for the project team members to interact.
The Core Advisory Committee represents the broad spectrum of expertise required for the successful operation of the HTS Core. This committee reviews all submitted proposals and ranks them based on their scientific merits and feasibility. Priorities are assigned to each proposal, and core staff members carry out the experiments accordingly. Highest priority is given to cancer center members with peer-reviewed funding.
A diagram showing processes and relationships within the HTS Core.
