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Confocal Microscopes

Confocal Microscopes
For more information on one of the microscopes listed below, please click on the appropriate tab above.
 
  • Zeiss Upright LSM510 2-Photon Microscope
  • Zeiss Inverted LSM510 META 2-Photon Microscope
  • Zeiss Upright LSM310 Laser Scanning Confocal Microscope
  • Prarie Technologies Ultima 2-Photon Microscope and Coherent Chameleon Ultra II Laser

Scheduling Equipment   
 
 
 

Confocal Microscope Images

Human vascular smooth muscle cells stained with caveolin-1 and phalloidin and HOECHST dye for nucleus. One of the stains was red and other was green. This image is a merged image of the two stains and HOECHST dye.
 
Reddy, Marpadga, Assistant Research Scientist, Diabetes and Endocrinology, City of Hope
   
Acini formation of human prostate epithelial cell line, RWPE-1, grown on top of Matri gel for seven days in the presence of prostate epithelial growth medium plus several growth factors cocktail. This picture was taken under a 20x objective lens.
 
Chen, Charng-Jui (Christine), Assistant Research Scientist, Immunology, City of Hope
   
Transfection of Mutant Ephrin Bs SiRNA to neurophrogenitor cells. Red = Nestin, green = transfected cells, blue = Hoechst nuclear stain.
 
Runxiang Qiu, Research Fellow, Neurosciences, City of Hope
   
Ephrin B1 and its reverse signaling mediator = pdz-RGS3 co-expressed in E16 mouse cerebral cortex VZ (40x). Green = Anti GFP, red = Anti-RFP, blue = Hoechst, yellow = colocalization of red and green.
 
Runxiang Qiu, Research Fellow, Neurosciences, City of Hope
   
Ephrin B1 gain of function construct expression pattern in E16 mouse cerebral cortex (10x). Green = Anti-GFP, red = nestin, blue = Hoechst.
 
Runxiang Qiu, Research Fellow, Neurosciences, City of Hope
   
The expression in E16 mouse cerebral cortex of PDZ-RGS3s Ephrin B1’s Dominant Negative Construact (EF-EGFP-ARGS) injected into E13.5 mouse cerebral cortex (10x). Red = EGFP after staining, blue = Hoechst nuclear stain.
 
Runxiang Qiu, Research Fellow, Neurosciences, City of Hope
   
Plasmid expressing DsRed driven by CDK5r promoter into the mouse embryo cerebral cortex on embryonic stage E13.5 by “in utero electroporation”, then dissected the brain and did coronal section on E15.5. The green staining was done by using anti-doublecortin antibody. The blue was Hoechst staining.
 
Wang, Xiuyun, Research Fellow, Neurosciences, City of Hope


Last updated: 7/1/08

Zeiss Upright LSM510 2-Photon Microscope

 
Widefield Imaging Modes
  • Bright Field
  • Nomarski DIC
  • Fluorescence
     

Laser Scanning Mode

  • Argon Laser 458, 488, 514 nm
  • HeNe Laser 543 nm
  • Tunable Ti-Sapphire IR Pulse Laser 730-950 nm for 2-Photon illumination
  • 2 PMT Detectors
  • 2 Non-Descanned PMT Detectors
  • 1 Transmitted Light PMT Detector
     

Imaging Features

  • Motorized stage: XY movements for tiling, Z for optical sectioning
  • Single or multitrack image collection
  • 8 or 12 bit pixel depth
  • 128, 256, 1024, 2048 pixel frame
  • Ratio Imaging
  • 3D projection from image stack, depth code, stereo pairs, red and green stereo images
  • Multi Time Macro Time Series control
  • Physiology Macro
  • LSM 4.2 on Windows XP Platform
     

Configuration

Dyes

  • Click on tab above to view examples of the fluorescent dyes available for confocal microscopes
     

Confocal Microscope Images

  • Click on tab above to view examples of confocal images shot with the available microscopes.
     

Objectives

Last updated: 2013

Zeiss LSM510 META NLO Axiovert 200M Inverted Microscope

 

Widefield Microscopy Modes

  • Bright Field
  • Nomarski DIC
  • Fluorescence
     

Laser Scanning Modes

  • Argon Laser 458, 488, 514 nm
  • HeNe 1 Laser 543 nm
  • HeNe 2 Laser 633 nm
  • Tunable IR Ti-Sapphire Pulse Laser 730-950nm (2-Photon Excitation)
  • 3 PMT detectors with individually controlled pinholes
  • 2 Non-Descanned Detectors
  • META polychromic 32 channel Spectral Detector
  • Transmitted light mode
     

Imaging Features

  • Motorized Stage; XY movement for tiling, Z for optical sectioning
  • Single or Multi-track image collection
  • 8 or 12 bit pixel depth
  • Lambda stack scanning
  • Linear Unmixing of spectral scans
  • Emission fingerprinting
  • Multiple ROI scanning
  • 3D projection from image stack, depth code, stereo pairs, red and green stereo images
  • 128, 256, 512, 1024, and 2048 pixel frame size
  • Ratio imaging
  • Multi-Time Macro for time lapse imaging
  • Physiology Macro
  • Continuously adjustable digital zoom
  • Detector rotation
  • Monitor Diode Detector reduces laser noise
  • Overlay of DIC or phase on fluorescent image
  • LSM 4.2 on Windows XP Platform
  • Other: an accessory incubator that controls temperature and CO2 levels during time lapse imaging of growing cells and tissue
     

Configuration

Dyes

  • Click on tab above to view dye list.
     

Confocal Microscope Images

  • Click on tab above to view confocal images.
     

Objectives

Last updated: 2013

 

Zeiss Inverted LSM 700 Microscope

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Widefield Visual Modes
• Bright Field
• DIC
• RGB Fluorescence: Exfo Xcite-120
 
Available Solid State LASERs
• 405nm
• 488nm
• 555nm
• 635nm
 
Detectors
• 2 Detectors with Spectral VSD (variable secondary dichroic)
• Transmitted Light Detector
 
Mounting Frames
• Universal Frame K for Slides
• Universal Frame KM for Plates
 
Imaging Features
• Observer Z1 Automated Stand
• Motorized: scanning stage for XY movements for tiling, Z for optical sectioning
• Single or multitrack image collection
• 8 or 12 or 16 bit pixel depth
• 128, 256, 1024, 2048 pixels per frame
• Zen 2012 Imaging Software for Acquisition and Analysis
 
Objectives
• Fluar 10x/0.5NA M27
• EC Plan-Neofluar 20x/0.5NA M27
• LD LCI 25x/0.8NA Water Imm Corr M27
• Plan-Apo 40x/0.95NA Corr M27
• iLCI Plan Neofluar 63x/1.3 Water Imm Corr M27
 

Prairie Technologies Ultima 2-Photon Microscope

 

Prairie Ultima

 

  • Objectives; 10x/0.3 Dry WD 10mm,
  • 20xW/1.0 Imm (XLUMPLN) WD 2mm,
  • 40xW/IR-2/0.8 Imm WD 3.3mm,
  • 60xW/IR-2/0.8 Imm WD 3.3mm
  • Transmitted and Fluorescence Illumination (Exfo Xcite)
  • Upright Configuration
  • Custom Heated Stage Insert
     

Laser Scanning Mode

  • Galvo; for high quality imaging
  • AOD; for fast imaging, e.g., 25 fps
  • 4 Above Stage PMT Detectors (primary)
  • 2 Substage Detectors (secondary)
     

Imaging Features

  • Motorized stage: X, Y, and Z for optical sectioning
  • 16 bit TIFF images
  • 128, 256, 512, 1024, 2048 pixel resolution
  • Frame Averaging
  • Adjustable Dwell Time
  • Advanced Time Series and Z-Sectioning
  • Variable Zoom
     

Coherent Laser

  • Coherent Chameleon Ultra II
  • Fast Tuning from 680nm - 1080nm
  • Power output approximately 3000mW at 800nm
  • Capable of exciting all fluorophores in the visible spectrum
     

Spectra

Last updated: 2013

 

Fluorescent Dyes for Confocal Microscopes

Laser Line Excitation Dyes Emission Color
790 nm IR Ti-Sapphire Hoechst 33342
Dapi
AlexaFluor-350
Cascade Blue
Violet/blue
457 nm Argon Lucifer Yellow Green/yellow
488 nm Argon AlexaFluor-488
Oregon Green
YOYO-1 for DNA
Cy-2
FITC
CFSE
Green
Green
Green
Green
Green
Green
543 nm HeNe
AlexaFluor-546
AlexaFluar-555
AlexaFluor-568
Tetramethyl Rhodamine
Lisamine Rhodamine
Propidium Iodiine
BOBO-1 for DNA
Red
633 nm HeNe
Cy-5
Cy-5.5
AlexaFluor-633
AlexaFluar-647
TOTO-3 for DNA
 
Far Red

Last updated: 2013
City of Hope
Light Microscopy Digital Imaging Core
1500 East Duarte Road
Furth Building
Room F1113
Duarte, CA 91010
626-301-8870 phone
626-301-8908 fax
DIC Striated Muscle
Click here for more information on our Widefield Light Microscopes.
Developing fruit fly nervous system
Click here for more information on our Confocal Microscopes.
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City of Hope embodies the spirit of scientific collaboration by sharing services and core facilities with colleagues here and around the world.
 

Beckman Research Institute of City of Hope is internationally  recognized for its innovative biomedical research.
City of Hope is one of only 41 Comprehensive Cancer Centers in the country, the highest designation awarded by the National Cancer Institute to institutions that lead the way in cancer research, treatment, prevention and professional education.
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