A National Cancer Institute-designated Comprehensive Cancer Center

Make an appointment: 800-826-HOPE
Confocal Microscopes Bookmark and Share

Confocal Microscopes

For more information on one of the microscopes listed below, please click on the appropriate tab above.
 
  • Zeiss Upright LSM510 2-Photon Microscope
  • Zeiss Inverted LSM510 META 2-Photon Microscope
  • Zeiss Inverted LSM700
  • Prarie Technologies Ultima 2-Photon Microscope and Coherent Chameleon Ultra II Laser

Scheduling Equipment   
 
 
 

Confocal Microscope Images

Human vascular smooth muscle cells stained with caveolin-1 and phalloidin and HOECHST dye for nucleus. One of the stains was red and other was green. This image is a merged image of the two stains and HOECHST dye.
 
Reddy, Marpadga, Assistant Research Scientist, Diabetes and Endocrinology, City of Hope
   
Acini formation of human prostate epithelial cell line, RWPE-1, grown on top of Matri gel for seven days in the presence of prostate epithelial growth medium plus several growth factors cocktail. This picture was taken under a 20x objective lens.
 
Chen, Charng-Jui (Christine), Assistant Research Scientist, Immunology, City of Hope
   
Transfection of Mutant Ephrin Bs SiRNA to neurophrogenitor cells. Red = Nestin, green = transfected cells, blue = Hoechst nuclear stain.
 
Runxiang Qiu, Research Fellow, Neurosciences, City of Hope
   
Ephrin B1 and its reverse signaling mediator = pdz-RGS3 co-expressed in E16 mouse cerebral cortex VZ (40x). Green = Anti GFP, red = Anti-RFP, blue = Hoechst, yellow = colocalization of red and green.
 
Runxiang Qiu, Research Fellow, Neurosciences, City of Hope
   
Ephrin B1 gain of function construct expression pattern in E16 mouse cerebral cortex (10x). Green = Anti-GFP, red = nestin, blue = Hoechst.
 
Runxiang Qiu, Research Fellow, Neurosciences, City of Hope
   
The expression in E16 mouse cerebral cortex of PDZ-RGS3s Ephrin B1’s Dominant Negative Construact (EF-EGFP-ARGS) injected into E13.5 mouse cerebral cortex (10x). Red = EGFP after staining, blue = Hoechst nuclear stain.
 
Runxiang Qiu, Research Fellow, Neurosciences, City of Hope
   
Plasmid expressing DsRed driven by CDK5r promoter into the mouse embryo cerebral cortex on embryonic stage E13.5 by “in utero electroporation”, then dissected the brain and did coronal section on E15.5. The green staining was done by using anti-doublecortin antibody. The blue was Hoechst staining.
 
Wang, Xiuyun, Research Fellow, Neurosciences, City of Hope


Last updated: 7/1/08

Zeiss Upright LSM510 2-Photon Microscope

 
Widefield Imaging Modes
  • Bright Field
  • Nomarski DIC
  • Fluorescence
     

Laser Scanning Mode

  • Argon Laser 458, 488, 514 nm
  • HeNe Laser 543 nm
  • Tunable Ti-Sapphire IR Pulse Laser 730-950 nm for 2-Photon illumination
  • 2 PMT Detectors
  • 2 Non-Descanned PMT Detectors
  • 1 Transmitted Light PMT Detector
     

Imaging Features

  • Motorized stage: XY movements for tiling, Z for optical sectioning
  • Single or multitrack image collection
  • 8 or 12 bit pixel depth
  • 128, 256, 1024, 2048 pixel frame
  • Ratio Imaging
  • 3D projection from image stack, depth code, stereo pairs, red and green stereo images
  • Multi Time Macro Time Series control
  • Physiology Macro
  • LSM 4.2 on Windows XP Platform
     

Configuration

Dyes

  • Click on tab above to view examples of the fluorescent dyes available for confocal microscopes
     

Confocal Microscope Images

  • Click on tab above to view examples of confocal images shot with the available microscopes.
     

Objectives

Last updated: 2013

Zeiss LSM510 META NLO Axiovert 200M Inverted Microscope

 

Widefield Microscopy Modes

  • Bright Field
  • Nomarski DIC
  • Fluorescence
     

Laser Scanning Modes

  • Argon Laser 458, 488, 514 nm
  • HeNe 1 Laser 543 nm
  • HeNe 2 Laser 633 nm
  • Tunable IR Ti-Sapphire Pulse Laser 730-950nm (2-Photon Excitation)
  • 3 PMT detectors with individually controlled pinholes
  • 2 Non-Descanned Detectors
  • META polychromic 32 channel Spectral Detector
  • Transmitted light mode
     

Imaging Features

  • Motorized Stage; XY movement for tiling, Z for optical sectioning
  • Single or Multi-track image collection
  • 8 or 12 bit pixel depth
  • Lambda stack scanning
  • Linear Unmixing of spectral scans
  • Emission fingerprinting
  • Multiple ROI scanning
  • 3D projection from image stack, depth code, stereo pairs, red and green stereo images
  • 128, 256, 512, 1024, and 2048 pixel frame size
  • Ratio imaging
  • Multi-Time Macro for time lapse imaging
  • Physiology Macro
  • Continuously adjustable digital zoom
  • Detector rotation
  • Monitor Diode Detector reduces laser noise
  • Overlay of DIC or phase on fluorescent image
  • LSM 4.2 on Windows XP Platform
  • Other: an accessory incubator that controls temperature and CO2 levels during time lapse imaging of growing cells and tissue
     

Configuration

Dyes

  • Click on tab above to view dye list.
     

Confocal Microscope Images

  • Click on tab above to view confocal images.
     

Objectives

Last updated: 2013

 

Zeiss LSM 700 Confocal Microscope

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Widefield Visual Modes
• Bright Field
• DIC
• RGB Fluorescence: Exfo Xcite-120
 
Available Solid State LASERs
• 405nm
• 488nm
• 555nm
• 635nm
 
Detectors
• 2 Detectors with Spectral VSD (variable secondary dichroic)
• Transmitted Light Detector
 
Mounting Frames
• Universal Frame K for Slides
• Universal Frame KM for Plates
 
Imaging Features
• Observer Z1 Automated Stand
• Motorized: scanning stage for XY movements for tiling, Z for optical sectioning
• Single or multitrack image collection
• 8 or 12 or 16 bit pixel depth
• 128, 256, 1024, 2048 pixels per frame
• Zen 2012 Imaging Software for Acquisition and Analysis
 
Objectives
• Fluar 10x/0.5NA M27
• EC Plan-Neofluar 20x/0.5NA M27
• LD LCI 25x/0.8NA Water Imm Corr M27
• Plan-Apo 40x/0.95NA Corr M27
• iLCI Plan Neofluar 63x/1.3 Water Imm Corr M27
 

Prairie Technologies Ultima 2-Photon Microscope

 

Prairie Ultima

 

  • Objectives; 10x/0.3 Dry WD 10mm,
  • 20xW/1.0 Imm (XLUMPLN) WD 2mm,
  • 40xW/IR-2/0.8 Imm WD 3.3mm,
  • 60xW/IR-2/0.8 Imm WD 3.3mm
  • Transmitted and Fluorescence Illumination (Exfo Xcite)
  • Upright Configuration
  • Custom Heated Stage Insert
     

Laser Scanning Mode

  • Galvo; for high quality imaging
  • AOD; for fast imaging, e.g., 25 fps
  • 4 Above Stage PMT Detectors (primary)
  • 2 Substage Detectors (secondary)
     

Imaging Features

  • Motorized stage: X, Y, and Z for optical sectioning
  • 16 bit TIFF images
  • 128, 256, 512, 1024, 2048 pixel resolution
  • Frame Averaging
  • Adjustable Dwell Time
  • Advanced Time Series and Z-Sectioning
  • Variable Zoom
     

Coherent Laser

  • Coherent Chameleon Ultra II
  • Fast Tuning from 680nm - 1080nm
  • Power output approximately 3000mW at 800nm
  • Capable of exciting all fluorophores in the visible spectrum
     

Spectra

Last updated: 2013

 

Fluorescent Dyes for Confocal Microscopes

Laser Line Excitation Dyes Emission Color
790 nm IR Ti-Sapphire Hoechst 33342
Dapi
AlexaFluor-350
Cascade Blue
Violet/blue
457 nm Argon Lucifer Yellow Green/yellow
488 nm Argon AlexaFluor-488
Oregon Green
YOYO-1 for DNA
Cy-2
FITC
CFSE
Green
Green
Green
Green
Green
Green
543 nm HeNe
AlexaFluor-546
AlexaFluar-555
AlexaFluor-568
Tetramethyl Rhodamine
Lisamine Rhodamine
Propidium Iodiine
BOBO-1 for DNA
Red
633 nm HeNe
Cy-5
Cy-5.5
AlexaFluor-633
AlexaFluar-647
TOTO-3 for DNA
 
Far Red

Last updated: 2013

Confocal Microscopes

Confocal Microscopes

For more information on one of the microscopes listed below, please click on the appropriate tab above.
 
  • Zeiss Upright LSM510 2-Photon Microscope
  • Zeiss Inverted LSM510 META 2-Photon Microscope
  • Zeiss Inverted LSM700
  • Prarie Technologies Ultima 2-Photon Microscope and Coherent Chameleon Ultra II Laser

Scheduling Equipment   
 
 
 

Confocal Microscope Images

Confocal Microscope Images

Human vascular smooth muscle cells stained with caveolin-1 and phalloidin and HOECHST dye for nucleus. One of the stains was red and other was green. This image is a merged image of the two stains and HOECHST dye.
 
Reddy, Marpadga, Assistant Research Scientist, Diabetes and Endocrinology, City of Hope
   
Acini formation of human prostate epithelial cell line, RWPE-1, grown on top of Matri gel for seven days in the presence of prostate epithelial growth medium plus several growth factors cocktail. This picture was taken under a 20x objective lens.
 
Chen, Charng-Jui (Christine), Assistant Research Scientist, Immunology, City of Hope
   
Transfection of Mutant Ephrin Bs SiRNA to neurophrogenitor cells. Red = Nestin, green = transfected cells, blue = Hoechst nuclear stain.
 
Runxiang Qiu, Research Fellow, Neurosciences, City of Hope
   
Ephrin B1 and its reverse signaling mediator = pdz-RGS3 co-expressed in E16 mouse cerebral cortex VZ (40x). Green = Anti GFP, red = Anti-RFP, blue = Hoechst, yellow = colocalization of red and green.
 
Runxiang Qiu, Research Fellow, Neurosciences, City of Hope
   
Ephrin B1 gain of function construct expression pattern in E16 mouse cerebral cortex (10x). Green = Anti-GFP, red = nestin, blue = Hoechst.
 
Runxiang Qiu, Research Fellow, Neurosciences, City of Hope
   
The expression in E16 mouse cerebral cortex of PDZ-RGS3s Ephrin B1’s Dominant Negative Construact (EF-EGFP-ARGS) injected into E13.5 mouse cerebral cortex (10x). Red = EGFP after staining, blue = Hoechst nuclear stain.
 
Runxiang Qiu, Research Fellow, Neurosciences, City of Hope
   
Plasmid expressing DsRed driven by CDK5r promoter into the mouse embryo cerebral cortex on embryonic stage E13.5 by “in utero electroporation”, then dissected the brain and did coronal section on E15.5. The green staining was done by using anti-doublecortin antibody. The blue was Hoechst staining.
 
Wang, Xiuyun, Research Fellow, Neurosciences, City of Hope


Last updated: 7/1/08

Zeiss Upright LSM510 2-Photon Microscope

Zeiss Upright LSM510 2-Photon Microscope

 
Widefield Imaging Modes
  • Bright Field
  • Nomarski DIC
  • Fluorescence
     

Laser Scanning Mode

  • Argon Laser 458, 488, 514 nm
  • HeNe Laser 543 nm
  • Tunable Ti-Sapphire IR Pulse Laser 730-950 nm for 2-Photon illumination
  • 2 PMT Detectors
  • 2 Non-Descanned PMT Detectors
  • 1 Transmitted Light PMT Detector
     

Imaging Features

  • Motorized stage: XY movements for tiling, Z for optical sectioning
  • Single or multitrack image collection
  • 8 or 12 bit pixel depth
  • 128, 256, 1024, 2048 pixel frame
  • Ratio Imaging
  • 3D projection from image stack, depth code, stereo pairs, red and green stereo images
  • Multi Time Macro Time Series control
  • Physiology Macro
  • LSM 4.2 on Windows XP Platform
     

Configuration

Dyes

  • Click on tab above to view examples of the fluorescent dyes available for confocal microscopes
     

Confocal Microscope Images

  • Click on tab above to view examples of confocal images shot with the available microscopes.
     

Objectives

Last updated: 2013

Zeiss LSM510 META NLO Axiovert 200M Inverted Microscope

Zeiss LSM510 META NLO Axiovert 200M Inverted Microscope

 

Widefield Microscopy Modes

  • Bright Field
  • Nomarski DIC
  • Fluorescence
     

Laser Scanning Modes

  • Argon Laser 458, 488, 514 nm
  • HeNe 1 Laser 543 nm
  • HeNe 2 Laser 633 nm
  • Tunable IR Ti-Sapphire Pulse Laser 730-950nm (2-Photon Excitation)
  • 3 PMT detectors with individually controlled pinholes
  • 2 Non-Descanned Detectors
  • META polychromic 32 channel Spectral Detector
  • Transmitted light mode
     

Imaging Features

  • Motorized Stage; XY movement for tiling, Z for optical sectioning
  • Single or Multi-track image collection
  • 8 or 12 bit pixel depth
  • Lambda stack scanning
  • Linear Unmixing of spectral scans
  • Emission fingerprinting
  • Multiple ROI scanning
  • 3D projection from image stack, depth code, stereo pairs, red and green stereo images
  • 128, 256, 512, 1024, and 2048 pixel frame size
  • Ratio imaging
  • Multi-Time Macro for time lapse imaging
  • Physiology Macro
  • Continuously adjustable digital zoom
  • Detector rotation
  • Monitor Diode Detector reduces laser noise
  • Overlay of DIC or phase on fluorescent image
  • LSM 4.2 on Windows XP Platform
  • Other: an accessory incubator that controls temperature and CO2 levels during time lapse imaging of growing cells and tissue
     

Configuration

Dyes

  • Click on tab above to view dye list.
     

Confocal Microscope Images

  • Click on tab above to view confocal images.
     

Objectives

Last updated: 2013

 

Zeiss Inverted LSM 700 Microscope

Zeiss LSM 700 Confocal Microscope

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Widefield Visual Modes
• Bright Field
• DIC
• RGB Fluorescence: Exfo Xcite-120
 
Available Solid State LASERs
• 405nm
• 488nm
• 555nm
• 635nm
 
Detectors
• 2 Detectors with Spectral VSD (variable secondary dichroic)
• Transmitted Light Detector
 
Mounting Frames
• Universal Frame K for Slides
• Universal Frame KM for Plates
 
Imaging Features
• Observer Z1 Automated Stand
• Motorized: scanning stage for XY movements for tiling, Z for optical sectioning
• Single or multitrack image collection
• 8 or 12 or 16 bit pixel depth
• 128, 256, 1024, 2048 pixels per frame
• Zen 2012 Imaging Software for Acquisition and Analysis
 
Objectives
• Fluar 10x/0.5NA M27
• EC Plan-Neofluar 20x/0.5NA M27
• LD LCI 25x/0.8NA Water Imm Corr M27
• Plan-Apo 40x/0.95NA Corr M27
• iLCI Plan Neofluar 63x/1.3 Water Imm Corr M27
 

Prairie Technologies Ultima 2-Photon Microscope

Prairie Technologies Ultima 2-Photon Microscope

 

Prairie Ultima

 

  • Objectives; 10x/0.3 Dry WD 10mm,
  • 20xW/1.0 Imm (XLUMPLN) WD 2mm,
  • 40xW/IR-2/0.8 Imm WD 3.3mm,
  • 60xW/IR-2/0.8 Imm WD 3.3mm
  • Transmitted and Fluorescence Illumination (Exfo Xcite)
  • Upright Configuration
  • Custom Heated Stage Insert
     

Laser Scanning Mode

  • Galvo; for high quality imaging
  • AOD; for fast imaging, e.g., 25 fps
  • 4 Above Stage PMT Detectors (primary)
  • 2 Substage Detectors (secondary)
     

Imaging Features

  • Motorized stage: X, Y, and Z for optical sectioning
  • 16 bit TIFF images
  • 128, 256, 512, 1024, 2048 pixel resolution
  • Frame Averaging
  • Adjustable Dwell Time
  • Advanced Time Series and Z-Sectioning
  • Variable Zoom
     

Coherent Laser

  • Coherent Chameleon Ultra II
  • Fast Tuning from 680nm - 1080nm
  • Power output approximately 3000mW at 800nm
  • Capable of exciting all fluorophores in the visible spectrum
     

Spectra

Last updated: 2013

 

Fluorescent Dyes for Confocal Microscopes

Fluorescent Dyes for Confocal Microscopes

Laser Line Excitation Dyes Emission Color
790 nm IR Ti-Sapphire Hoechst 33342
Dapi
AlexaFluor-350
Cascade Blue
Violet/blue
457 nm Argon Lucifer Yellow Green/yellow
488 nm Argon AlexaFluor-488
Oregon Green
YOYO-1 for DNA
Cy-2
FITC
CFSE
Green
Green
Green
Green
Green
Green
543 nm HeNe
AlexaFluor-546
AlexaFluar-555
AlexaFluor-568
Tetramethyl Rhodamine
Lisamine Rhodamine
Propidium Iodiine
BOBO-1 for DNA
Red
633 nm HeNe
Cy-5
Cy-5.5
AlexaFluor-633
AlexaFluar-647
TOTO-3 for DNA
 
Far Red

Last updated: 2013
City of Hope
Light Microscopy Digital Imaging Core
1500 East Duarte Road
Furth Building
Room F1113
Duarte, CA 91010
626-301-8870 phone
626-301-8908 fax
DIC Striated Muscle
Click here for more information on our Widefield Light Microscopes.
Developing fruit fly nervous system
Click here for more information on our Confocal Microscopes.
Research Shared Services

City of Hope embodies the spirit of scientific collaboration by sharing services and core facilities with colleagues here and around the world.
 

Beckman Research Institute of City of Hope is internationally  recognized for its innovative biomedical research.
City of Hope is one of only 41 Comprehensive Cancer Centers in the country, the highest designation awarded by the National Cancer Institute to institutions that lead the way in cancer research, treatment, prevention and professional education.
NEWS & UPDATES
  • September is Prostate Cancer Awareness Month. Here, Bertram Yuh, M.D., assistant clinical professor in the Division of Urology and Urologic Services at City of Hope, explains the importance of understanding the risk factors for the disease and ways to reduce those risks, as well as overall prostate health. “Wha...
  • ** Learn more about prostate health, plus prostate cancer research and treatment, at City of Hope. ** Learn more about getting a second opinion at City of Hope by visiting us online or by calling 800-826-HOPE (4673). City of Hope staff will explain what’s required for a consult at City of Hope and help yo...
  • Childhood cancer survival rates have increased dramatically over the past 40 years. More than 80 percent of children with cancer now survive five years or more, which is a tremendous feat. Despite the survival rate increase, cancer continues to be the No. 1 disease killer and second-leading cause of death in ch...
  • Although a stem cell transplant can be a lifesaving procedure for people diagnosed with a blood cancer or blood disorder, the standard transplant may not be appropriate for all patients. This is because the conditioning regimen (the intensive chemotherapy and/or radiation treatments preceding the transplant) is...
  • Brain tumor removal would seem to be the obvious course of action in the wake of a brain tumor diagnosis, but that’s not always the case. Some tumors are too difficult for many surgeons to reach or too close to areas that control vital functions. Removing them just proves too risky. A new device being con...
  • Hijacking the same sorts of viruses that cause HIV and using them to reprogram immune cells to fight cancer sounds like stuff of the future. Some scientists believe that the future is closer than we think – and are now studying the approach in clinical trials at City of Hope. Immunotherapy is a promising approa...
  • Jennifer Linehan, M.D., an assistant clinical professor in City of Hope’s Division of Urology and Urologic Oncology in Antelope Valley, thought she knew all there was to know about treating prostate cancer. Then her father was diagnosed with the disease. This is her story. ** My father is 69 years old, has no h...
  • Nausea is the one of the most well-known, and dreaded, side effects of cancer treatment — and with good reason. Beyond the quality-of-life issues that it causes, severe nausea can prevent patients from receiving enough nutrients and calories at a time when they need every edge they can get. A few simple actions...
  • With Labor Day just around the corner, summer is on its way out. But just because summertime is ending doesn’t mean we can skip sunscreen. Protection from ultraviolet (UV) radiation is needed all year round. Exposure to UV radiation — whether from the sun or from artificial sources such as sunlamps used i...
  • Undergoing reconstructive surgery may seem like a forgone conclusion for survivors of breast cancer, but that doesn’t appear to be the case. A new study has found that most breast cancer survivors who undergo a mastectomy decide against surgical reconstruction of their breasts. The reasons for such a deci...
  • Nearly four decades ago, City of Hope began its bone marrow transplant program. Its first transplant reunion celebration was a single patient and his donor, also his brother. This year, City of Hope welcomed hundreds of hematopoietic cell transplant (HCT) recipients to the annual bone marrow transplant/HCT reun...
  • The burgeoning type 2 diabetes epidemic casts a pall over the health of America’s public. New research now shows the looming threat is getting worse. Much worse. A diabetes trends study published earlier this month in the Lancet Diabetes and Endocrinology by researchers at the federal Centers for Disease Contro...
  • An aspirin a day might help keep breast cancer away for some breast cancer survivors, a new study suggests. Obese women who have had breast cancer could cut their risk of a recurrence in half if they regularly take aspirin or other nonsteroidal anti-inflammatory drugs, called NSAIDs, report researchers from the...
  • Christine Crews isn’t only a fitness enthusiast, she’s also a personal trainer and fitness instructor. Being active defines her life. So when she was diagnosed with bladder cancer at age 30, she decided she absolutely couldn’t let the disease interfere with that lifestyle. And it didn’t. For t...
  • Cancer treatment and the cancer itself can cause changes in your sense of taste or smell. These side effects typically subside after treatment ends, but there are ways to help alleviate those bitter and metallic tastes in your mouth. Here are tips from the National Cancer Institute to help keeps tastes and food...