Dr. Lamparska is currently a Postdoctoral Fellow in Dr. Steven S. Smith's lab. Her work is in biochemistry and bionanotechnology, involving IDA and NTA chemistry and the construction of modified oligomers including FAM, TAMRA probes for the detection of prostate cancer by QPCR.
TAQMAN probes are synthesized by standard phase b -cyanoethyl-phosphoramidite chemistry on 1 m mol scale on a Cyclone Plus DNA Synthesizer. Tamra was attached to the 3-amino-modifed oligomer using post-synthesis labeling technique. Fam was attached to the oligomer using the standard phosphoamidite cycles of DNA synthesis. After oligonucleotide deprotection (standard ammonium conditions) the DNA was purified on HPLC using reversed-phase chromatography.
Dr. Lamparska graduated from Adam Mickiewicz University in Poznan, Poland in 1997 with a master’s degree in chemistry. Her master’s thesis was based on a novel method for the synthesis of 5-F-Uridine phosphoramidite. She received her Ph.D. in chemistry in 2003 at the same University with the thesis: “The synthesis and photochemistry of 5-halogeno-4-thiouracil analogues of RNA in the studies of DNA-RNA and RNA-peptide photocrosslinking.” These molecules were used in the structural analysis of nucleic acids and the their interaction with protein.