Equipment


NEW! Zeiss Sigma VP Field Emission Scanning Electron Microscope (FE-SEM) with Gemini column.
This FE-SEM is equipped several detectors for secondary (SE) and backscattered (BSE) electrons.  These include in-lens SE, variable pressure SE, Everhart-Thornley SE, BSE, and scanning transmission electron microscopy (STEM) detectors.  Also available is a large window (150 mm2) Oxford X-MAX SDD X-ray detector available for identifying and mapping elements within specimens.
 
 
NEW! Gatan 3View2XP In-chamber microtome.
The 3View2XP in-chamber microtome, when combined with the Zeiss Sigma VP FE-SEM, produces three-dimensional images in a fully-automated, high-throughput, high-resolution fashion.  Data collected provide 3D data on the ultrastructure of cells and organelles and the opportunity for robust quantitative analyses at ultrastructural resolution.
 
 
FEI Tecnai Twin 120kV transmission electron microscope (TEM).
This TEM provides several modes for directly imaging cell and macromolecular ultrastructure. The TEM can be used to examine negatively-stained specimens, cryo-specimens and sections of resin-embedded specimens.  It is a equipped with a Gatan Ultrascan 1000 2K CCD camera.  A 70° tilt cryo-transfer holder enables frozen specimens to be imaged for tomography at temperatures below -170 °C. Software and hardware are available for acquiring and aligning tomography data for subsequent reconstruction and for single particle analysis.
 
 
Gatan sample holder and pumping station. 
These are used for cryo-specimens in the TEM.
 
 
FEI Quanta 200 Scanning Electron Microscope (SEM).
This SEM is a versatile, high-performance (tungsten filament) scanning electron microscope with three operating modes (high vacuum, low vacuum and ESEM) equipped with a motorized stage and detectors for secondary and backscattered electrons.
 
 
Bruker Multimode 8 Atomic Force Microscope (AFM).
Te AFM provides a means for imaging the surfaces of specimens using a physical probe. The Multimode 8 provides nearly all the major scanning microscopy imaging methods. These include contact and non-contact atomic force, lateral force, tapping (air), magnetic force, electric force microscopy and ScanAsyst (Peak Force Tapping) techniques. A cantilever holder is also available for scanning in fluid using tapping and force modulation modes.
 
NEW! This instrument has been upgraded with a ScanAsyst-HR high speed scanner offering 20X faster scanning.
 
 
 
New! PELCO Biowave Pro 36500 Laboratory Microwave System.
This instrument is available for specimen preparation allowing otherwise time-consuming sample preparation to be semi-automated and accomplished more quickly.
 
 
FEI Vitrobot Mark IV.
This fully automated vitrification device for plunge-freezing of aqueous (colloidal) suspensions provides a powerful means for determining the 3D structure of macromolecules and macromolecular complexes in their natural state. Single-particle analysis and EM reconstruction of specimens prepared with the Vitrobot and analyzed by Cryo-EM can partner nicely with X-ray crystallography and NMR spectrometry in determining of molecular structures.
 
 
Leica EM PACT2 High Pressure Freezer (HPF).
The HPF equipped with RTS allows ultra-rapid freezing of specimens. High pressure freezing is superior to chemical fixation for the preservation of the native structure of cells and molecules. Antigenic sites in specimens are better preserved with HPF, opening greater possibilities for localization of molecules of interest within cells. Also available is a Leica Microbiopsy Transfer System (not shown) which provides the means for rapidly collecting microbiopsy specimens.
 
 
Leica EM Automatic Freeze Substitution System.
Freezing substitution is the follow-on procedure for high pressure freezing. Specimens are fixed and resin-infiltrated at low temperature providing higher quality structural preservation including specimens suitable for immunolabeling for electron microscopy
 
 
Leica Ultracut UCT Microtome for ultrathin sectioning. 
This versatile ultramicrotome is the workhorse instrument in the EM Core. It provides sections of resin-embedded specimens prepared by conventional and cryo methods. An FCS attachment allows low temperature ultrathin sectioning of biological samples at temperatures as low as -185°C following the method of Tokuyasu.
 
 
Leica VT1000S Vibrating-blade microtome, commonly called a vibratome.
The vibrating razor blade in the vibratome provides relatively thick sections (~100 microns) suitable for specialized techniques of electron microscopy including immuno-EM.
 
 
Cressington 308 Coating System
This coating system provides thermal evaporation of various metals.  It is most often used for sputter coating SEM samples and for depositing carbon onto TEM grids.
 
 
Polaron Critical Point Dryer.
Critical point drying provides better preservation of specimens for scanning electron microscopy.