Ultra-thin Section from Resin Embedded Samples for TEM
The most commonly used approach to obtaining ultrastructural details of cells and tissues.
Cryo-EM of Plunge-Frozen Samples
Frozen-hydrated liposomes.
Toluidine Blue Stained 1-um-thick Section
One-micron sections provide an overview as illustrated here with a colony of pancreatic stem cells.
Negative Staining
Negative stained exosomes.
High Pressure Freezing and Freeze Substitution
Compared to harsh chemical fixation, HPF-FS provides superior preservation of cellular structure.
Immuno-gold labeling on specimen prepared by high pressure freezing/freeze substitution
Indirect immunolabeling with antibody conjugated 10-nm gold particles was used to monitor uptake of FITC-CpG-siRNA.
Immunogold Labeling SEM
In the SEM the immunogold particles are bright. Here immunogold labeling reveals CEACAM-1 associated with microvilli on the cell surface.
Visualization of Nucleic Acid
Rotary shadowing of spread DNA
Scanning Electron Microscopy
Surface structure of a monocyte
Atomic Force Microscopy
Structure of mRNA molecules (~1000 nt)

Transmission Electron Microscopy
Ultra-thin section of avian LMH cells prepared with membrane-enhancing reagents.
Transmission Electron Microscopy
Ultra-thin section of an avian LMH cell. Sample preparation was done on an adhesive slide using only ~2000 cells. This method can be used to image small numbers of cells (for example, directly from flow cytometry).
Transmission Electron Microscopy
Single wall carbon nanotubes with a diameter of about 1.4 nm resolved in the Tecnai 12 transmission electron microscope.