A National Cancer Institute-designated Comprehensive Cancer Center

Make an appointment: 800-826-HOPE
High Throughput Screening Bookmark and Share

High Throughput Screening Core

Mission
The High Throughput Screening (HTS) Core’s mission is to assist City of Hope researchers in identifying novel lead compounds for anticancer drug development and chemical probes useful for biochemical mechanistic studies via sophisticated high throughput screening assays of synthetic compound and natural product libraries.
 
A team of medicinal chemists, pharmacologists, structural biologists, bioinformaticians and clinicians contributes valuable input to optimize some of these small molecules for drug development and translate them into clinical evaluation in cancer patients. 
 
Compound Library
The core has a library of over 75,000 synthetic compounds that represent prime leads for drug development, as well as a library of over 7,000 natural product extracts comprising nearly 1 million individual compounds.
 
HTS Core Features Summary and Advantages
The HTS Core is a full-time, professionally-managed facility, boasting highly-trained and experienced staff, advanced equipment and a substantial compound library. These combined attributes and a centralized knowledge base increase cost effectiveness and productivity. Investigators also benefit from the cumulative experience gleaned from other projects in the High Throughput Screening Core.
 
Research reported in this publication included work performed in the High Throughput Screening (HTS) Core supported by the National Cancer Institute of the National Institutes of Health under award number P30CA33572. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Interaction Between Cores

The following image shows interactions between the High Throughput Screening (green), Biomedical Informatics (yellow) and Synthetic and Biopolymer Chemistry (purple).
 
Experimental screenings performed in the Highthroughput ScreeningCore complement virtual screenings carried out by the Biomedical Informatics Core toward identifying “hits” for biomolecular targets of interest.
 
Confirmed hits, also known as leads, identified through screenings are used as starting points for chemists in the Synthetic and Biopolymer Chemistry Core for designing focused libraries. 
Molecular modeling expertise in the Biomedical Informatics Core aids the design process.
 
Chemists synthesize the focused libraries accordingly, which in turn are tested by high throughput screening.
 
Structure-activity relationship analysis is then carried out to evaluate newly synthesized compounds using appropriate in vitro or cell-based assays.
 
Iterative cycles of interactions of the three cores will speed up the “lead optimization” process and move the project forward.
 

Pricing

Current service offering and pricing can be found on our iLab site. Please contact us for further questions.
 

Abstract for Grants

City of Hope researchers identify novel lead compounds for anticancer drug development and chemical probes useful for biochemical mechanistic studies via sophisticated high throughput screening (HTS) assays of synthetic compound and natural product libraries.
 
A team of medicinal chemists, pharmacologists, structural biologists, bioinformaticians and clinicians contributes valuable input to optimize some of these small molecules for drug development and translate them into clinical evaluation in cancer patients.
 
The HTS Core works closely with the Synthetic and Biopolymer Chemistry Core and the Biomedical Informatics Core in the identification, improvement and development of lead compounds, which in turn benefit chemical biology and drug discovery research at City of Hope.
 
The HTS Core facility occupies approximately 600 square feet of laboratory space in the newly-renovated Flower Research Building, which is five minutes from the main City of Hope campus. The lab has ample space for major robotic equipment, computers, freezers, a microplate washer and reader, tissue culture and benchtop working space. The core’s major equipment includes a Hudson Platecrane, a Beckman Biomek FX, a Tecan Ultra Plate Reader, a BioTek Elx405 Select Auto Plate Washer, a a Liconic Plate Incubator, Thermo Multidrop Micro, a Biacore T100 Surface Plasmon Resonance Biosensor and SoftLinx software.
 
The HTS Core is a full-time, professionally-managed facility, boasting highly-trained and experienced staff, advanced equipment and a substantial compound library. These combined attributes and a centralized knowledge base increase cost effectiveness and productivity. It would not be feasible or cost effective for individual investigators to acquire the necessary expertise and instruments to perform their own HTS. Investigators also benefit from the cumulative experience gleaned from other projects in the HTS Core.
 
The HTS Core has deployed a series of informatics programs and implemented standard operating procedures to ensure proper management of the large amount of information it handles regularly. These steps also foster efficient information exchange between the core and its users.

Equipment

The High Throughput Screening Core facility occupies approximately 600 square feet of laboratory space in the Flower Research Building, and contains major robotic equipment, computers, freezers, a microplate washer and reader, tissue culture and benchtop working space.
 
The major equipment is described in more detail below.
 
Programmable rotary robotic plate handler that shuffles microtiter plates between specified instrument positions and plate racks.
 
Beckman Biomek® FX
The Biomek FX Laboratory Workstation is a flexible, automated liquid handling system with a large work surface accommodating almost any highthroughput job size. The system comes configured with both 96-channel and Span-8 multichannel pipetting capabilities, simplifying highthroughput automation in 96- or 384-well format, with up to a 240-plate capacity with the Stacker Carousel. Multichannel pipetting makes for rapid compound transfer and addition of reagents. Automated labware positioners allow the researcher to customize the work surface with automated functionality such as shaking, stirring, heating and cooling for a given application; the integrated gripper frees the user from routine tasks like clearing the work surface to make room for more plates. An automated tip loader and Stacker Carousel storage likewise automate the routine task of providing disposable labware to the highthroughput system. The Biomek FX software is easy to use and possesses features for precise and accurate pipetting, as well as automation tools such as the Software Wizard, making it simple to write methods to automate any task.
 
Overlord™
Special supervisory software that allows the control of multiple instruments from one PC, Overlord operates in Windows 2000/XP environments with an easy-to-use drag-and-drop interface. Overlord utilizes the native software of ancillary equipment for maximum flexibility. Even the most complex system is easy to program and the most complex instrumentation is easy to integrate. There are over 300 Overlord installations in the field. Device drivers for Overlord are available for more than 300 instruments, and we use it to integrate the six instruments listed here into a screening platform.
 
Tecan ULTRA Plate Reader
Microplate reader capable of handling all fluorescence, luminescence, absorbance, fluorescence polarization and time-resolved fluorescence detection technologies.
 
In addition to managing detection technologies similar to those handled by Tecan Ultra, PHERAstar is equipped to handle the bead-based Amplified Luminescent Proximity Homogeneous Assay (AlphaScreen) technology patented by PerkinElmer.
 
BioTek Elx405™ Select Auto Plate Washer
A plate washer that is capable of washing 96- and 384-well microtiter plates. Users can adjust the flow rate, the dispense/aspiration volume and angle for applications from basic ELISA to cell washings.
 
LiCONiC Plate Incubator
Integrated incubator to allow screening with cell-based assays.
 
Thermo Multidrop Micro
Multidrop Micro is a high-speed 1-µl bulk reagent dispenser that precisely delivers microvolumes of 1-50 µl into 384- and 96-well microplates. It is especially useful forhigh throughput screeningassays set up with rare and/or expensive components.
 
Biacore T100 Surface Plasmon Resonance (SPR) Biosensor
State-of-the-art instrument for characterizing specific interactions of protein with other molecules such as proteins, synthetic and natural chemicals. The rates at which these molecules interact (binding and dissociation), and theiraffinity(how tightly they bind to another molecule) can be obtained easily.
 

Future Plans for HTS Core Development

Large-scale Protein Expression
Having sufficient quantities of reagents is a basic prerequisite to successful high-throughput screening (HTS). Often, supplies of target proteins are limited because most academic labs do not have the capability to produce the necessary amount of proteins. We plan to consult with the experts in City of Hope’s Center for Biomedicine & Genetics to establish large-scale protein expression capability to improve the protein supply bottleneck.
 
Addition of High Content Screening (HCS) Capability
Carrying out highthroughput cell-based “phenotypic” screening (HCS) requires integrated instrumentation and informatics infrastructures. These include automated digital microscopy, image analysis and data mining software, as well as a data management package. These infrastructures allow researchers to measure multiple end points/parameters simultaneously for every sample. They can then use advanced algorithms to dissect and distill useful information from the complex data set. We believe HCS capability will advance drug discovery on multiple fronts, including target identification and validation, primary and secondary screening, compound profiling and lead optimization.
 
Development of In Silico ADME Prediction and In Vitro ADME Testing
One of the major obstacles in drug discovery and development is the high failure rate of compounds during clinical trials. Many of the compounds fail because of poor absorption, distribution, metabolization and excretion (ADME) properties. With the advancement of computational power and methodologies, it is now possible to performin silicocalculation of a number of important ADME properties including aqueous solubility, log P octane/water, Caco-2 cell permeability and human serum albumin binding. By combining thein silicocalculation within vitroADME testing to confirm the predicted values, we can more accurately target selection of lead compounds for further development. The Biomedical Informatics Core will purchase ADME prediction software and make it available through City of Hope’s intranet. The HTS Core will establishin vitroADME assays, including Biomimetic Artificial Membrane Permeation Assay (BAMPA), Transil Human Serum Albumin (HSA) Binding Assay and Cytochrome P450 Isoenzymes Assay, to obtain experimental data on key ADME properties for compounds of interest.

Quality Control

By setting up the High Throughput Screening Core (HTS) in-house, we can provide timely assistance to City of Hope investigators regarding HTS assays development, validation and implementation. This allows for centralized quality control of drug screening and development. In addition, the HTS Core works closely with the Synthetic and Biopolymer Chemistry Core and the Biomedical Informatics Core in identifying, improving and developing lead compounds, which in turn benefit chemical biology and drug discovery research at City of Hope.
 
The HTS Core has deployed a series of informatics programs and implemented standard operating procedures to ensure proper management of the large amount of information it handles regularly. These steps also foster efficient information exchange between the core and its users.

 
Laboratory Information Management System
The HTS Core has set up a centralized database, built upon the Laboratory Information Management System developed by Cheminnovation called Chemical and Biological Information System (CBIS).
 
CBIS provides a Web-based application for managing chemical and biological materials and data throughout different cores and laboratory scientists, enabling insights resulting from high-volume data mining.
 
CBIS is hosted on the Biomedical Informatics Core’s high-availability Dell PowerEdge 2950, two dual-core 64-bit Xeon servers with 4GB RAM each and 1-terabyte SAN network storage space. CBIS’s integrated open framework of Web solutions has a user-friendly front end that can incorporate many external data mining applications and transparently link to relational database back end solutions.
 
Compound Registration
All compounds, regardless of source, are entered into the database and assigned a unique ID number, which facilitates tracking of the compound as it passes through various assays and tests.
 
Barcoding
All HTS assay plates use barcodes to allow tracking of the plates and data as compounds are tested in different assays.
 
Documentation
Reproducibility and robustness of each HTS assay is documented, e.g. by Z-factor analysis.
 
Statistical Analysis
S-PLUSsoftware, from Insightful Inc., is an industry-standard solution for exploratory data modeling and statistical analysis. With over 4,000 data analysis functions, including the most comprehensive and robust set of modern methods, S-PLUS is used on all data to ensure its quality and significance.
 
Information Exchange/Sharing
All high quality data is deposited into the central database in a timely manner to facilitate information exchange/sharing and discussions among researchers.

Services

A diagram showing processes within the HTS core.
The High Throughput Screening (HTS) Core facility offers the following services:
 
Consultation
Discussing with investigators the various approaches in identifying/screening compounds
 
Assay Development
Assisting investigators in developing new assays or adapting their low throughput assays into automated, high throughput screening formats. Assays may involve proteins, cell lines, or simple model organisms.
 
Assay Implementation
The core subjects the assays to high throughput screening and analyzes the results to identify active hits for each assay.
 
Data Analysis
Performing structure-activity relationship (SAR) analysis of compounds demonstrating activity in the individual assays.
 
Data Integration and Management
The core assists in collecting, maintaining and interpreting data for project investigators.
 
Drug Discovery: A Team Approach
Drug discovery is a multi-disciplinary effort. In order to advance a project efficiently, it is important that everyone involved be up-to-date with the progress of the project such as the latest hits/leads. It is also crucial to form a consensus about the characteristics of the compounds, e.g. potency, selectivity, etc., so that the team can effectively decide whether to go further with a particular compound and/or its derivatives. The HTS Core services provide an effective framework for the project team members to interact.
 
TheCore Advisory Committeerepresents the broad spectrum of expertise required for the successful operation of the HTS Core. This committee reviews all submitted proposals and ranks them based on their scientific merits and feasibility. Priorities are assigned to each proposal, and core staff members carry out the experiments accordingly. Highest priority is given to cancer center members with peer-reviewed funding.
 

High Throughput Screening

High Throughput Screening Core

Mission
The High Throughput Screening (HTS) Core’s mission is to assist City of Hope researchers in identifying novel lead compounds for anticancer drug development and chemical probes useful for biochemical mechanistic studies via sophisticated high throughput screening assays of synthetic compound and natural product libraries.
 
A team of medicinal chemists, pharmacologists, structural biologists, bioinformaticians and clinicians contributes valuable input to optimize some of these small molecules for drug development and translate them into clinical evaluation in cancer patients. 
 
Compound Library
The core has a library of over 75,000 synthetic compounds that represent prime leads for drug development, as well as a library of over 7,000 natural product extracts comprising nearly 1 million individual compounds.
 
HTS Core Features Summary and Advantages
The HTS Core is a full-time, professionally-managed facility, boasting highly-trained and experienced staff, advanced equipment and a substantial compound library. These combined attributes and a centralized knowledge base increase cost effectiveness and productivity. Investigators also benefit from the cumulative experience gleaned from other projects in the High Throughput Screening Core.
 
Research reported in this publication included work performed in the High Throughput Screening (HTS) Core supported by the National Cancer Institute of the National Institutes of Health under award number P30CA33572. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Interaction Between Cores

Interaction Between Cores

The following image shows interactions between the High Throughput Screening (green), Biomedical Informatics (yellow) and Synthetic and Biopolymer Chemistry (purple).
 
Experimental screenings performed in the Highthroughput ScreeningCore complement virtual screenings carried out by the Biomedical Informatics Core toward identifying “hits” for biomolecular targets of interest.
 
Confirmed hits, also known as leads, identified through screenings are used as starting points for chemists in the Synthetic and Biopolymer Chemistry Core for designing focused libraries. 
Molecular modeling expertise in the Biomedical Informatics Core aids the design process.
 
Chemists synthesize the focused libraries accordingly, which in turn are tested by high throughput screening.
 
Structure-activity relationship analysis is then carried out to evaluate newly synthesized compounds using appropriate in vitro or cell-based assays.
 
Iterative cycles of interactions of the three cores will speed up the “lead optimization” process and move the project forward.
 

Pricing

Pricing

Current service offering and pricing can be found on our iLab site. Please contact us for further questions.
 

Abstract

Abstract for Grants

City of Hope researchers identify novel lead compounds for anticancer drug development and chemical probes useful for biochemical mechanistic studies via sophisticated high throughput screening (HTS) assays of synthetic compound and natural product libraries.
 
A team of medicinal chemists, pharmacologists, structural biologists, bioinformaticians and clinicians contributes valuable input to optimize some of these small molecules for drug development and translate them into clinical evaluation in cancer patients.
 
The HTS Core works closely with the Synthetic and Biopolymer Chemistry Core and the Biomedical Informatics Core in the identification, improvement and development of lead compounds, which in turn benefit chemical biology and drug discovery research at City of Hope.
 
The HTS Core facility occupies approximately 600 square feet of laboratory space in the newly-renovated Flower Research Building, which is five minutes from the main City of Hope campus. The lab has ample space for major robotic equipment, computers, freezers, a microplate washer and reader, tissue culture and benchtop working space. The core’s major equipment includes a Hudson Platecrane, a Beckman Biomek FX, a Tecan Ultra Plate Reader, a BioTek Elx405 Select Auto Plate Washer, a a Liconic Plate Incubator, Thermo Multidrop Micro, a Biacore T100 Surface Plasmon Resonance Biosensor and SoftLinx software.
 
The HTS Core is a full-time, professionally-managed facility, boasting highly-trained and experienced staff, advanced equipment and a substantial compound library. These combined attributes and a centralized knowledge base increase cost effectiveness and productivity. It would not be feasible or cost effective for individual investigators to acquire the necessary expertise and instruments to perform their own HTS. Investigators also benefit from the cumulative experience gleaned from other projects in the HTS Core.
 
The HTS Core has deployed a series of informatics programs and implemented standard operating procedures to ensure proper management of the large amount of information it handles regularly. These steps also foster efficient information exchange between the core and its users.

Equipment

Equipment

The High Throughput Screening Core facility occupies approximately 600 square feet of laboratory space in the Flower Research Building, and contains major robotic equipment, computers, freezers, a microplate washer and reader, tissue culture and benchtop working space.
 
The major equipment is described in more detail below.
 
Programmable rotary robotic plate handler that shuffles microtiter plates between specified instrument positions and plate racks.
 
Beckman Biomek® FX
The Biomek FX Laboratory Workstation is a flexible, automated liquid handling system with a large work surface accommodating almost any highthroughput job size. The system comes configured with both 96-channel and Span-8 multichannel pipetting capabilities, simplifying highthroughput automation in 96- or 384-well format, with up to a 240-plate capacity with the Stacker Carousel. Multichannel pipetting makes for rapid compound transfer and addition of reagents. Automated labware positioners allow the researcher to customize the work surface with automated functionality such as shaking, stirring, heating and cooling for a given application; the integrated gripper frees the user from routine tasks like clearing the work surface to make room for more plates. An automated tip loader and Stacker Carousel storage likewise automate the routine task of providing disposable labware to the highthroughput system. The Biomek FX software is easy to use and possesses features for precise and accurate pipetting, as well as automation tools such as the Software Wizard, making it simple to write methods to automate any task.
 
Overlord™
Special supervisory software that allows the control of multiple instruments from one PC, Overlord operates in Windows 2000/XP environments with an easy-to-use drag-and-drop interface. Overlord utilizes the native software of ancillary equipment for maximum flexibility. Even the most complex system is easy to program and the most complex instrumentation is easy to integrate. There are over 300 Overlord installations in the field. Device drivers for Overlord are available for more than 300 instruments, and we use it to integrate the six instruments listed here into a screening platform.
 
Tecan ULTRA Plate Reader
Microplate reader capable of handling all fluorescence, luminescence, absorbance, fluorescence polarization and time-resolved fluorescence detection technologies.
 
In addition to managing detection technologies similar to those handled by Tecan Ultra, PHERAstar is equipped to handle the bead-based Amplified Luminescent Proximity Homogeneous Assay (AlphaScreen) technology patented by PerkinElmer.
 
BioTek Elx405™ Select Auto Plate Washer
A plate washer that is capable of washing 96- and 384-well microtiter plates. Users can adjust the flow rate, the dispense/aspiration volume and angle for applications from basic ELISA to cell washings.
 
LiCONiC Plate Incubator
Integrated incubator to allow screening with cell-based assays.
 
Thermo Multidrop Micro
Multidrop Micro is a high-speed 1-µl bulk reagent dispenser that precisely delivers microvolumes of 1-50 µl into 384- and 96-well microplates. It is especially useful forhigh throughput screeningassays set up with rare and/or expensive components.
 
Biacore T100 Surface Plasmon Resonance (SPR) Biosensor
State-of-the-art instrument for characterizing specific interactions of protein with other molecules such as proteins, synthetic and natural chemicals. The rates at which these molecules interact (binding and dissociation), and theiraffinity(how tightly they bind to another molecule) can be obtained easily.
 

Future Plans

Future Plans for HTS Core Development

Large-scale Protein Expression
Having sufficient quantities of reagents is a basic prerequisite to successful high-throughput screening (HTS). Often, supplies of target proteins are limited because most academic labs do not have the capability to produce the necessary amount of proteins. We plan to consult with the experts in City of Hope’s Center for Biomedicine & Genetics to establish large-scale protein expression capability to improve the protein supply bottleneck.
 
Addition of High Content Screening (HCS) Capability
Carrying out highthroughput cell-based “phenotypic” screening (HCS) requires integrated instrumentation and informatics infrastructures. These include automated digital microscopy, image analysis and data mining software, as well as a data management package. These infrastructures allow researchers to measure multiple end points/parameters simultaneously for every sample. They can then use advanced algorithms to dissect and distill useful information from the complex data set. We believe HCS capability will advance drug discovery on multiple fronts, including target identification and validation, primary and secondary screening, compound profiling and lead optimization.
 
Development of In Silico ADME Prediction and In Vitro ADME Testing
One of the major obstacles in drug discovery and development is the high failure rate of compounds during clinical trials. Many of the compounds fail because of poor absorption, distribution, metabolization and excretion (ADME) properties. With the advancement of computational power and methodologies, it is now possible to performin silicocalculation of a number of important ADME properties including aqueous solubility, log P octane/water, Caco-2 cell permeability and human serum albumin binding. By combining thein silicocalculation within vitroADME testing to confirm the predicted values, we can more accurately target selection of lead compounds for further development. The Biomedical Informatics Core will purchase ADME prediction software and make it available through City of Hope’s intranet. The HTS Core will establishin vitroADME assays, including Biomimetic Artificial Membrane Permeation Assay (BAMPA), Transil Human Serum Albumin (HSA) Binding Assay and Cytochrome P450 Isoenzymes Assay, to obtain experimental data on key ADME properties for compounds of interest.

Quality Control

Quality Control

By setting up the High Throughput Screening Core (HTS) in-house, we can provide timely assistance to City of Hope investigators regarding HTS assays development, validation and implementation. This allows for centralized quality control of drug screening and development. In addition, the HTS Core works closely with the Synthetic and Biopolymer Chemistry Core and the Biomedical Informatics Core in identifying, improving and developing lead compounds, which in turn benefit chemical biology and drug discovery research at City of Hope.
 
The HTS Core has deployed a series of informatics programs and implemented standard operating procedures to ensure proper management of the large amount of information it handles regularly. These steps also foster efficient information exchange between the core and its users.

 
Laboratory Information Management System
The HTS Core has set up a centralized database, built upon the Laboratory Information Management System developed by Cheminnovation called Chemical and Biological Information System (CBIS).
 
CBIS provides a Web-based application for managing chemical and biological materials and data throughout different cores and laboratory scientists, enabling insights resulting from high-volume data mining.
 
CBIS is hosted on the Biomedical Informatics Core’s high-availability Dell PowerEdge 2950, two dual-core 64-bit Xeon servers with 4GB RAM each and 1-terabyte SAN network storage space. CBIS’s integrated open framework of Web solutions has a user-friendly front end that can incorporate many external data mining applications and transparently link to relational database back end solutions.
 
Compound Registration
All compounds, regardless of source, are entered into the database and assigned a unique ID number, which facilitates tracking of the compound as it passes through various assays and tests.
 
Barcoding
All HTS assay plates use barcodes to allow tracking of the plates and data as compounds are tested in different assays.
 
Documentation
Reproducibility and robustness of each HTS assay is documented, e.g. by Z-factor analysis.
 
Statistical Analysis
S-PLUSsoftware, from Insightful Inc., is an industry-standard solution for exploratory data modeling and statistical analysis. With over 4,000 data analysis functions, including the most comprehensive and robust set of modern methods, S-PLUS is used on all data to ensure its quality and significance.
 
Information Exchange/Sharing
All high quality data is deposited into the central database in a timely manner to facilitate information exchange/sharing and discussions among researchers.

Services

Services

A diagram showing processes within the HTS core.
The High Throughput Screening (HTS) Core facility offers the following services:
 
Consultation
Discussing with investigators the various approaches in identifying/screening compounds
 
Assay Development
Assisting investigators in developing new assays or adapting their low throughput assays into automated, high throughput screening formats. Assays may involve proteins, cell lines, or simple model organisms.
 
Assay Implementation
The core subjects the assays to high throughput screening and analyzes the results to identify active hits for each assay.
 
Data Analysis
Performing structure-activity relationship (SAR) analysis of compounds demonstrating activity in the individual assays.
 
Data Integration and Management
The core assists in collecting, maintaining and interpreting data for project investigators.
 
Drug Discovery: A Team Approach
Drug discovery is a multi-disciplinary effort. In order to advance a project efficiently, it is important that everyone involved be up-to-date with the progress of the project such as the latest hits/leads. It is also crucial to form a consensus about the characteristics of the compounds, e.g. potency, selectivity, etc., so that the team can effectively decide whether to go further with a particular compound and/or its derivatives. The HTS Core services provide an effective framework for the project team members to interact.
 
TheCore Advisory Committeerepresents the broad spectrum of expertise required for the successful operation of the HTS Core. This committee reviews all submitted proposals and ranks them based on their scientific merits and feasibility. Priorities are assigned to each proposal, and core staff members carry out the experiments accordingly. Highest priority is given to cancer center members with peer-reviewed funding.
 
Research Shared Services

City of Hope embodies the spirit of scientific collaboration by sharing services and core facilities with colleagues here and around the world.
 

Recognized nationwide for its innovative biomedical research, City of Hope's Beckman Research Institute is home to some of the most tenacious and creative minds in science.
City of Hope is one of only 41 Comprehensive Cancer Centers in the country, the highest designation awarded by the National Cancer Institute to institutions that lead the way in cancer research, treatment, prevention and professional education.
Learn more about City of Hope's institutional distinctions, breakthrough innovations and collaborations.
Support Our Research
By giving to City of Hope, you support breakthrough discoveries in laboratory research that translate into lifesaving treatments for patients with cancer and other serious diseases.
 
 
 
 
Media Inquiries/Social Media
 
CONNECT WITH US
Facebook  Twitter  YouTube  Blog
 


NEWS & UPDATES
  • The outlook and length of survival has not changed much in the past 25 years for patients suffering from an aggressive form of pancreatic cancer known as pancreatic ductal adenocarcinoma (PDAC). These patients still have few options for therapy; currently available therapies are generally toxic and do not incre...
  • “With bladder cancer, the majority of patients that I see can be cured,” said urologist Kevin Chan, M.D., head of reconstructive urology at City of Hope. “The challenge is to get patients the same quality of life that they had before surgery.” To meet this challenge, Chan and the urologic team at City of Hope [...
  • Already pioneers in the use of immunotherapy, City of Hope researchers are now testing the bold approach to cancer treatment against one of medicine’s biggest challenges: brain cancer. This month, they will launch a clinical trial using patients’ own modified T cells to fight advanced brain tumors. One of but a...
  • Brain cancer may be one of the most-frightening diagnoses people can receive, striking at the very center of who we are as individuals. Further, it often develops over time, causing no symptoms until it’s already advanced. Listen to City of Hope Radio as Behnam Badie, M.D., director of the Brain Tumor Pro...
  • The whole is greater than the sum of its parts. It takes a village. No man is an island. Choose your aphorism: It’s a simple truth that collaboration usually is better than isolation. That’s especially true when you’re trying to introduce healthful habits and deliver health care to people at risk of disease and...
  • When Maryland Governor Larry Hogan announced earlier this week that he has the most common form of non-Hodgkin lymphoma, he was giving voice to the experience of more than 71,000 Americans each year. The announcement came with Hogan’s promise to stay in office while undergoing aggressive treatment for the...
  • The spine can be affected by many different kinds of tumors. Malignant, or cancerous, tumors can arise within the spine itself. Secondary spinal tumors, which are actually much more common, begin as cancers in another part of the body, such as the breast and prostate, and then spread, or metastasize, to the spi...
  • Although most cancer occurs in older adults, the bulk of cancer research doesn’t focus on this vulnerable and fast-growing population. City of Hope and its Cancer and Aging Research Team aim to change that, and they’re getting a significant boost from Professional Practice Leader Peggy Burhenn, R.N....
  • Liz Graef-Larcher’s first brain tumor was discovered by accident six years ago. The then-48-year-old with a long history of sinus problems and headaches had been sent for an MRI, and the scan found a lesion in her brain called a meningioma – a tumor that arises in the meninges, the layers of tissue that cover a...
  • The colon and rectum are parts of the body’s gastrointestinal system, also called the digestive tract. After food is digested in the stomach and nutrients are absorbed in the small intestine, the remaining material moves down into the lower large intestine (colon) where water and nutrients are absorbed. The low...
  • If there is one truism about hospital stays it is that patients want to get out. For many, however, the joy of being discharged is tempered by the unexpected challenges that recovery in a new setting may pose. Even with professional help, the quality of care and treatment that patients receive at City of Hope [...
  • Jana Portnow, M.D., associate director of the Brain Tumor Program at City of Hope, didn’t expect to specialize in treating brain tumors. But, early in her career, she undertook a year of research on pain management and palliative care and, in that program, got to know many patients with brain tumors. After that...
  • Ask any patient: Nurses are as pivotal in their care as doctors. They answer the call of a patient in the middle of the night, they hold the patient’s hand as he or she takes on yet another round of treatment and, in the best-case scenario, they wave goodbye as the patient leaves the hospital, […]
  • Many oncologists, not to mention their patients, might think that there’s no place for mathematical analysis in the treatment of cancer. They might think that all treatment decisions are based on unique factors affecting individual patients, with no connection to other patients and their treatment regimen...
  • Within three days in 2007, Stephanie Hosford, then 37, learned that she was pregnant with her long-awaited second child – and that she had triple-negative breast cancer. Soon afterward, Hosford discovered that she and her husband, Grant, had been approved to adopt a little girl from China.  After encountering m...