City of Hope researchers identify novel lead compounds for anticancer drug development and chemical probes useful for biochemical mechanistic studies via sophisticated high throughput screening (HTS) assays of synthetic compound and natural product libraries.
A team of medicinal chemists, pharmacologists, structural biologists, bioinformaticians and clinicians contributes valuable input to optimize some of these small molecules for drug development and translate them into clinical evaluation in cancer patients.
The HTS Core works closely with the Synthetic and Biopolymer Chemistry Core and the Biomedical Informatics Core in the identification, improvement and development of lead compounds, which in turn benefit chemical biology and drug discovery research at City of Hope.
The HTS Core facility occupies approximately 600 square feet of laboratory space in the newly-renovated Flower Research Building, which is five minutes from the main City of Hope campus. The lab has ample space for major robotic equipment, computers, freezers, a microplate washer and reader, tissue culture and benchtop working space. The core’s major equipment includes a Hudson Platecrane, a Beckman Biomek FX, a Tecan Ultra Plate Reader, a BioTek Elx405 Select Auto Plate Washer, a a Liconic Plate Incubator, Thermo Multidrop Micro, a Biacore T100 Surface Plasmon Resonance Biosensor and SoftLinx software.
The HTS Core is a full-time, professionally-managed facility, boasting highly-trained and experienced staff, advanced equipment and a substantial compound library. These combined attributes and a centralized knowledge base increase cost effectiveness and productivity. It would not be feasible or cost effective for individual investigators to acquire the necessary expertise and instruments to perform their own HTS. Investigators also benefit from the cumulative experience gleaned from other projects in the HTS Core.
The HTS Core has deployed a series of informatics programs and implemented standard operating procedures to ensure proper management of the large amount of information it handles regularly. These steps also foster efficient information exchange between the core and its users.