A National Cancer Institute-designated Comprehensive Cancer Center

Make an appointment: 800-826-HOPE

Light Microscopy Digital Imaging

Light Microscopy Digital Imaging
The objective of the Light Microscopy Digital Imaging Core is to provide services, facilities and expertise to City of Hope investigators needing high quality light microscope images.

Microscopy and imaging technology are rapidly evolving fields that depend increasingly on expensive equipment and skills beyond the means of most individual laboratories. We seek to provide a range of research-grade light and confocal microscope platforms for simple observations and documentation suitable for publication, education or grant proposals.

The Light Microscopy Digital Imaging Core also makes available the latest research platforms for automated microscopy experiments, advanced observation techniques and sophisticated digital image processing and analysis.

Note: This core is not supported by the National Cancer Institute-funded Cancer Center Support Grant.

  • Instruction in general aspects of light and confocal microscopy
  • Instruction in general aspects of digital imaging and image analysis
  • Instruction in operation of Light Microscopy Digital Imaging Core equipment
  • Consultation on microscopy and imaging projects

Last updated: 2013

Abstract for Grants

The objective of the City of Hope Light Microscopy Digital Imaging Core is to provide service, facilities and expertise to all City of Hope and Beckman Research Institute investigators who need high quality light microscopy images. The facility also seeks to provide state-of-the-art research platforms for automated microscopy experiments, advanced observation techniques and advanced digital image processing and analysis.
The Light Microscopy Digital Imaging Core has the following widefield light microscopes: Zeiss Observer Z1 for live-cell automated imaging in fluorescent or color imaging modes, Zeiss AxioVert 200 for fluorescent applications, Olympus AX70 upright for slide mounted specimens, Olympus IX81inverted for culture dish specimens, and an Olympus BX50 upright for fluorescent imaging. The faciity also has confocal microscopes that include: LSM510 2-Photon upright microscope, LSM510 META 2-Photon inverted microscope, LSM700 inverted microscope, and a Prairie Technologies Ultima 2-Photon microscope using a Coherent Chameleon Ultra II laser.
The Light Microscopy Digital Imaging Core is located in the Furth Building, Rooms F1113, F1114, and F1101, and the Prairie Microscope is located in Familian F1101A.

Imaging Software

  • AutoQuant, deconvolution software
  • Media Cybernetics, Image Pro Premier high-end image analysis software
  • Adobe Photoshop
  • Amira 3D Projection and Analysis software
  • AxioVision 4.8.2
  • Image J / Fiji
  • Zen 2012

Imaging Station

For demanding imaging applications such as 3D rendering, quantification, and analysis we provide a high-end imaging station.

Dell Precision T3500, Intel Xeon CPU, Windows 7 64-bit OS, 15GB RAM, and NVIDIA Quadro FX 5800 Video Card.

Programs include: Amira, Image Pro Premier, Zen 2012, LAS AF lite, MIPAV, Graph Pad, Photoshop CS5, Image J, FIJI, Reconstruct, Cell Profiler, Image Scope, and Blender.


Teaching Station

This station has a wheeled cart with wireless keyboard and mouse that can operate the computer from anywhere in the room. Computer is Dell Optiplex 7010 and wall mounted monitor is a 50" Sharp Plasma. This computer is networked and has Image Pro Premier as well as other imaging programs installed.

How to download Zen Lite 2012 Image Browser to view your images

  • Click on the link provided below.
  • http://microscopy.zeiss.com/microscopy/en_de/products/microscope-software/zen-lite.html#inpagetabs-0
  • Once you click on the link, you will be directed to the Zeiss Webpage.
  • Select the tab “Downloads”
  • Once in the “downloads” tab, locate and select “Register to download your copy of Zen lite free of charge” or you can click on the link provided to take you straight to the webpage. Register to download your copy of ZEN lite free of charge.
  • It will take you to a screen like the one below. Enter your information in the “Required Data” area.        
  • Select the “submit” button at the bottom of your screen.
  • An email will be sent with a download link to the email you provided.
  • Once you select the download link, you will be taken to the webpage (See below)
  • Choose the appropriate version (32-bit or 64-bit) for your computer by selecting the “Download via HTTP-protocol”             
  • Once you select the appropriate version, a window will appear prompting you to select “run” or “save.” Select “Save”
  • Name and Save the file in an appropriate folder.
  • A window will appear. Once it says “download complete,” select the “run” button.
  • You will be presented with a download wizard to install the program.
  • Follow the step by step instructructions until the download is complete.
  • Zen Lite 2012 will now be available on your computer or desktop.

Scheduling Equipment

In order to use the Light Microscopy Digital Imaging Core, you must receive training from the staff on the system you would like to use. Please contact us for training. All City of Hope users can reserve microscope times online through the Microsoft Outlook Calendar. Non-City of Hope certified users should contact us to reserve microscope time. Please contact us if you have any questions.
  • LSM510 Upright: MS Outlook/All Public Folders/Neurosciences/Confocal/LSM 510 Upright 2 Photon
  • LSM510 Inverted: MS Outlook/All Public Folders/Neurosciences/Confocal/LSM 510 Meta Inverted 2 Photon
  • LSM700 Inverted: MS Outlook/All Public Folders/Neurosciences/Confocal/LSM 700
  • Prairie Ultima 2-Photon: MS Outlook/All Public Folders/Neurosciences/Confocal/Prairie
  • Zeiss Observer: MS Outlook/All Public Folders/Neurosciences/Microscopy Core/Observer
  • Zeiss AxioVert 200: MS Outlook/All Public Folders/Neurosciences/Microscopy Core/AxioVert
  • 1X81 Inverted: MS Outlook/All Public Folders/Neurosciences/Microscopy Core/Inverted IX81
  • AX70 Upright: MS Outlook/All Public Folders/Neurosciences/Microscopy Core/AX70
  • BX50 Upright Mono: MS Outlook/All Public Folders/Neurosciences/Microscopy Core/Bx50


Service                                           User Rates

Confocal microscopy                   $75.00/hr 

Widefield microscopy                  $15.00/hr 

If you are a City of Hope employee, please click here for pricing.

Contact Us

Light Microscopy Digital Imaging Core   
City of Hope
1500 East Duarte Road
Furth Building
Room F1113
Duarte, CA 91010-3000

626-301-8870 phone
626-301-8908 fax
City of Hope
Light Microscopy Digital Imaging Core
1500 East Duarte Road
Furth Building
Room F1113
Duarte, CA 91010
626-301-8870 phone
626-301-8908 fax
DIC Striated Muscle
Click here for more information on our Widefield Light Microscopes.
Developing fruit fly nervous system
Click here for more information on our Confocal Microscopes.
Research Shared Services

City of Hope embodies the spirit of scientific collaboration by sharing services and core facilities with colleagues here and around the world.

Beckman Research Institute of City of Hope is internationally  recognized for its innovative biomedical research.
City of Hope is one of only 41 Comprehensive Cancer Centers in the country, the highest designation awarded by the National Cancer Institute to institutions that lead the way in cancer research, treatment, prevention and professional education.
  • Meet City of Hope’s new chair of the Department of Surgery – esteemed pancreatic and hepatobiliary surgeon, researcher and author Yuman Fong, M.D. As one of today’s most respected and recognizable physicians in the treatment of cancers of the liver, bile duct, gallbladder and pancreas, Fong has pioneered and en...
  • For most of her life, Southern California teenager Kayla Saikaly described herself as healthy, even very healthy. She played basketball. She never missed school with as much as a fever. Her worst childhood illness was nothing more than a cold. Then, when she was 13, her nose started bleeding after a basketball ...
  • Neuroblastoma is one of the deadliest childhood cancers, accounting for 15 percent of pediatric cancer deaths. For patients with high-risk neuroblastomas, the five-year survival rate is 40 to 50 percent even with the most rigorous treatments available today. But those odds may improve soon, thanks to a new comp...
  • For breast cancer survivors, a common worry is a recurrence of their cancer. Currently, these patients are screened with regular mammograms, but there’s no way to tell who is more likely to have a recurrence and who is fully cleared of her cancer. A new blood test – reported in Cancer Research, a journal of the...
  • Metastasis — the spreading of cancer cells from a primary tumor site to other parts of the body — generally leads to poorer outcomes for patients, so oncologists and researchers are constantly seeking new ways to detect and thwart this malicious process. Now City of Hope researchers may have identified a substa...
  • Deodorant, plastic bottles, grilled foods, artificial sweeteners, soy products … Do any of these products really cause cancer? With so many cancer myths and urban legends out there, why not ask the experts? They can debunk cancer myths while sharing cancer facts that matter, such as risk factors, preventi...
  • Cancer risk varies by ethnicity, as does the risk of cancer-related death. But the size of those differences can be surprising, highlighting the health disparities that exist among various ethnic groups in the United States. Both cancer incidence and death rates for men are highest among African-Americans, acco...
  • George Winston, known worldwide for his impressionistic, genre-defying music, considers music to be his first language, and admits he often stumbles over words – especially when he attempts languages other than English. There’s one German phrase he’s determined to perfect, however: danke schön. Winston thinks h...
  • Few decisions are more important than those involving health care, and few decisions can have such lasting impact, not only on oneself but on relatives and loved ones. Those choices, especially, should be made in advance – carefully, deliberately, free of pain and stress, and with much weighing of values and pr...
  • Using a card game to make decisions about health care, especially as those decisions relate to the end of life, would seem to be a poor idea. It isn’t. The GoWish Game makes those overwhelming, but all-important decisions not just easy, but natural. On each card of the 36-card deck is listed what seriously ill,...
  • Young adults and adolescents with cancer face unique challenges both during their treatment and afterward. Not only are therapies for children and older adults not always appropriate for them, they also must come to terms with the disease and treatment’s impact on their relationships, finances, school or ...
  • Breast cancer is the most common cancer, other than skin cancer, among women in the United States. It’s also the second-leading cause of cancer death, behind lung cancer. In the past several years, various task force recommendations and studies have questioned the benefits of broad screening guidelines fo...
  • Paternal age and the health effects it has on potential offspring have been the focus of many studies, but few have examined the effect parental age has on the risk of adult-onset hormone-related cancers (breast cancer, ovarian cancer and endometrial cancer). A team of City of Hope researchers, lead by Yani Lu,...
  • Hormone therapy, which is prescribed to women for relief of menopausal symptoms such hot flashes, night sweats and vaginal dryness, has recently seen a decline in popularity (and use) due to its link to an increased risk of breast and endometrial cancer. But City of Hope researchers have found that menopausal h...
  • Myeloproliferative neoplasms can’t be narrowed down to a single cancer, but they can be described by a defining characteristic: too many blood cells. The diseases bring with them a host of frustrating, potentially life-altering symptoms, and management of the diseases and their symptoms is crucial. An upcoming ...