The Mass Spectrometry and Proteomics Core is available on both a shared use (for internal users only) and full service basis. Investigators should contact the manager, Gabriel Gugiu at 626-256-HOPE (4673) ext. 63905 to discuss the experimental strategy for any sample analysis prior to sample submission.
The facility is available to City of Hope and Beckman Research Institute users on a shared use basis. Investigators wishing to use the facility on a shared use or collaborative basis should contact Gabriel Gugiu for more information.
How to Prepare Samples:
Please see the guidelines for preparing samples in order to obtain a successful analysis. Direct any concerns to the Mass Spectrometry and Proteomics manager, Gabriel Gugiu at 626-256-HOPE (4673) x63905 prior to sample submission.
With few exceptions (quantitation or method development standards), it is not necessary for the investigator to provide supplies for analyses and projects carried out by the Mass Spectrometry and Proteomics Core. The cost of these components is built into the fee schedule.
The turn-around time for the mass analysis of a single sample is one to two days for nano-electrospray MS on a simple mixture and 3-4 days for more complex samples. For samples submitted for LC/MS/MS analysis with database search and/or quantitation, typical turn-around time is about two weeks (backlog dependent). For samples that require method development, investigators will be apprised of the anticipated time required to obtain results of the requested analyses on a per sample basis when experimental details are discussed with the facility manager.
All users who wish to analyze their own samples must be trained by a member of the MS facility prior to their intended use of the instrument. Please contact us to schedule a training session.
Rules for instrument use:
Users must be trained in the specific technique and instrument they wish to use. Any other use must be discussed with the manager. Any fault observed with the instruments must be communicated to the facility personnel to promptly address the problem.
All instruments sign-up is handled through the online calendars in Microsoft Outlook which can be located in “Public Folder\All Public Folders\Mass Spectrometry core\” or through the direct links provided on this website. Note that the website links only work from Internet explorer. Please respect the signup rules below.
Instrument maintenance and repair takes precedence over other uses. Please discuss any concerns with us. We will attempt to accommodate you as best as we can.
The LTQ-FT is used for accurate mass determination for small molecule nano-electrospray peptide mass and sequence determinations and is available everyday with some exceptions noted below.
Thursdays after 4 PM are reserved for the LTQ-FT maintenance and calibration. No one may sign up on the LTQ-FT for more than 2 hours on Thursdays. Mondays are reserved for Synapt G2 HDMS and Agilent QQQ maintenance and calibration and Thursdays for Agilent Q-TOF maintenance and calibration.
LC/MS/MS time may be signed up for in 1-3-day blocks. If you need less time than that, sign up only for the number of days you need so that others can use the instrument. Exceptionally, more time can be reserved if the project requires it and if available, upon approval of the MS facility personnel.
For quantitation projects on any instrument it is allowed to reserve one-week block time (the time between two consecutive maintenance days).
No one may sign up for more than one week in any calendar month on a single instrument. For scheduling purposes, the first day of the time block determines its month.
No one group/laboratory may sign up for more than 6 consecutive days on one instrument in one month.
Please do not sign up more than 4 weeks ahead of time unless you have our permission because you need to coordinate with a collaborator who is visiting from off campus, or have lost your scheduled time due to unscheduled maintenance or repair work.
If any time blocks are not signed up a week in advance they can be used by anybody even if they are in violation of the above rules.