Contact Information

Professional Experience

  • 2009 - present, Professor, Department of Molecular Medicine, Beckman Research Institute of City of Hope, Duarte, CA
  • 2005 - 2009, Professor, Department of Immunology, Beckman Research Institute of City of Hope, Duarte, CA
  • 1999 - 2005, Associate Professor, Department of Immunology, Beckman Research Institute
  • 1994 - 1999, Assistant Professor, Department of Immunology, Beckman Research Institute

Education

Education/Training
  • 1992 - 1993, The Scripps Research Institute, CA, PostDoc, Structural Biology
  • 1992, Rutgers University/University of Medicine & Dentistry, NJ Ph.D., Biochemistry
  • 1985, University of Science & Technology of China, PRC. B.S., Chemistry

Honors/Awards

Awards
  • 1996, American Cancer Society Junior Faculty Research Award
  • 1993, NIH Postdoctoral Fellowship

Publications

  • Li Y, Perkins AL, Su Y, Ma Y, Colson L, Horne DA and Chen Y. Gold Nanoparticles as a Platform for Creating a Multi-valent Poly-SUMO Chain Inhibitor That Also Augments Ionizing Radiation. Proceeding of the National Academy of Sciences, in press.
  • Namanja AT, Li YJ, Su Y, Wong S, Lu J, Colson LT, Wu C, Li SS, Chen Y. Insights into high affinity SUMO recognition by SUMO-interacting motifs (SIM) revealed by a combination of NMR and peptide array analysis. J Biol Chem. 287(5):3231-40, 2012.
  • Cano KE, Li L, Bhatia S, Bhatia R, Forman SJ, and Chen Y. NMR-Based Metabolomic Analysis of the Molecular Pathogenesis of Therapy-Related Myelodysplasia/Acute Myeloid Leukemia. J Proteome Res, 10(6):2873-81, 2011.
  • Li Y, Stark J, Chen DJ, Ann DK and Chen Y. Role of SUMO:SIM-Mediated Protein-Protein Interaction in Non-Homologous End Joining, Oncogene, 17;29(24):3509-18, 2010.
  • Wang J, Hu W, Cai S, Lee B, Song J, Chen Y. The Intrinsic Affinity between E2 and the Cys Domain of E1 in Ubiquitin-like Modifications. Mol Cell. 2007 Jul 20;27(2):228-37. (With Preview by Haas AL. Structural Insights into Early Events in the Conjugation of Ubiquitin and Ubiquitin-like Proteins. Mol Cell. 2007 Jul 20;27(2):174-175.
  • Song J., Durrin LK, Wilkinson TA, Krontiris TG, and Chen Y. Identification of a SUMO Binding Motif that Recognizes SUMO Modified Proteins. Proceeding of the National Academy of Sciences 101(40), 14373-14378, 2004.
  • Khue Truong, Terry Lee, and Yuan Chen. SUMO modification of the E1 Cys domain inhibits its enzymatic activity. J. Biol. Chem. in press.
  • Weidong H, Namanja AT, Wong S, Chen Y. Selective Editing of Val and Leu Methyl Groups in High Molecular Weight Protein NMR, J. Bio NMR, in press
  • Gilbreth RN, Truong K, Madu I, Koide A, Wojcik J, Li N-S, Piccirilli JA, Chen Y and Koide S. Isoform-specific Monobody Inhibitors of SUMO/SIM Interactions Engineered Using Structure-guided Library Design. Proceeding of the National Academy of Sciences, 108(19):7751-6, 2011.
  • Buettner R, Corzano R, Rashid R, Lin J, Senthil M, Hedvat M, Schroeder A, Mao A, Herrmann A, Yim J, Li H, Yuan YC, Yakushijin K, Yakushijin F, Vaidehi N, Moore R, Gugiu G, Lee TD, Yip R, Chen Y, Jove R, Horne D, Williams JC. Alkylation of cysteine 468 in Stat3 defines a novel site for therapeutic development. ACS Chem Biol. 6(5):432-43, 2011.
  • Truong K, Su Y, Song J, Chen Y. Entropy Drive Mechanism of an E3 Ligase. Biochemistry, 50(25):5757-66, 2011.
  • Wang J and Chen Y. The Role of the Zn-Motif of E1 in SUMO Adenylation. J Biol Chem, 285(31):23732-8, 2010.
  • Cano KE, Li YJ, Chen Y. NMR metabolomic profiling reveals new roles of SUMOylation in DNA damage response. J Proteome Res. 9(10):5382-8, 2010.
  • Wang J, Cai S, Chen Y. Mechanism of E1-E2 interaction for the inhibition of Ubl adenylation. J Biol Chem, 285(43):33457-62, 2010.
  • Li Y, Stark J, Chen DJ, Ann DK and Chen Y. Role of SUMO:SIM-Mediated Protein-Protein Interaction in Non-Homologous End Joining, Oncogene, in press.
  • Tan J-A, Song J, Chen Y. and Durrin LK. Phosphorylation of the Genome Organizer, SATB1, Regulates its Interaction with the SUMO E3 Ligase Pias1, and Consequent Sub-nuclear Localization. Mol Cell Biol, 2010 Mar 29. [Epub ahead of print].
  • Song J, Wang J, Jozwiak AA, Hu W, Swiderski PM, and Chen Y. Stability of thioester intermediates in ubiquitin-like modifications, Protein Science, 18(12):2492-9, 2009.
  • Wang J, Lee B, Cai S, Fukui L, Hu W, Chen Y. Conformational transition associated with E1-E2 interaction in SUMO modifications, J Biol Chem. 284(30):20340-8, 2009.
  • Seu C and Chen Y. Identification of SUMO-binding motifs, Methods in Molecular Biology, 497, 121-140, 2009.
  • Cao X, Clavijo C, Li X, Lin HH, Chen Y, Shih HM, Ann DK. SUMOylation of HMGA2: selective destabilization of promyelocytic leukemia protein via proteasome. Mol Cancer Ther. 7(4):923-34, 2008.
  • Tan JA, Sun Y, Song J, Chen Y, Krontiris TG, Durrin LK. SUMO conjugation to the MAR-binding protein, special at-rich sequence binding protein-1, targets it to promyelocytic nuclear bodies where it undergoes caspase cleavage. Journal of Biological Chemistry, 283(26):18124-34, 2008.
  • Rouleau N, Wang J, Karras L, Andrews E, Bielefeld-Sevigny M, and Chen Y. Highly Sensitive Assays for Sumoylation and SUMO-Dependent Protein-Protein Interactions, Analytical Biochemistry, 375(2):364-6, 2008.
  • Sheng C, Seu C, Kovacs Z, Sherry AD and Chen Y. Sensitivity Enhancement of Multidimensional NMR Experiments by Paramagnetic Relaxation Effects. Journal of the American Chemical Society, in press.
  • Cai S, Zhu L, Zhang Z, Chen Y. Determination of the three-dimensional structure of the Mrf2-DNA complex using paramagnetic spin labeling. Biochemistry. 2007 May 1;46(17):4943-50.
  • Fukui L and Chen Y. NvMap: Automated Analysis of NMR chemical shift Perturbation Data. Bioinformatics. 2007 Feb 1;23(3):378-80
  • Cai S., Lee B, Cheng C, Hu W, Yoon J, Pfeifer GP and Chen Y. 1H, 13C and 15N resonance assignments of the C-terminal domain of RP2
  • Lee DH, Jin SG, Cai S, Chen Y, Pfeifer GP and O’Connor TR. Repair of methylation damage in DNA and RNA by mammalian AlkB homologues. J Biol Chem. 280(47):39448-59, 2005
  • Yoon J, Singh P, Lee DH, Qiu J, O’Connor TR, Chen Y, Shen B, and Pfeifer GP. Characterization of the 3’ to 5’ exonuclease activity found in human nucleoside diphosphate kinase 1 (NDK1) and several of its homologues. Biochemistry, 44(48):15774-86, 2005.
  • Yoon J, Qiu J, Cai S, Chen Y, Cheetham ME, Shen B, Pfeifer GP. The retinitis pigmentosa-mutated RP2 protein exhibits exonuclease activity and translocates to the nucleus in response to DNA damage. Experimental Cell Research, in press.
  • Wilkinson TA, Zhu L, Hu W, and Chen Y. Retention of Conformational Flexibility in High Affinity Protein-RNA Interactions. Biochemistry 43(51):16153-60, 2004.
  • Kim S, Zhang Z, Upchurch S, Isern N and Chen Y. Structure of the Human SWI1 ARID Reveals Determinants for Sequence-Specific DNA recognition. J. Biol Chem, 279(16):16670-6, 2004.
  • Wilsker D, Patsialou A, Zumbrun SD, Kim S, Chen Y, Dallas PB, Moran E. The DNA-binding properties of the ARID-containing subunits of yeast and mammalian SWI/SNF complexes. Nucleic Acids Res. 32(4):1345-53, 2004.
  • Zhang Z, Hu, W, Cano L, Lee TD, Chen D, and Chen Y. Solution Structure of the C-terminal Domain of Ku80 Suggests Important Sites for Protein-Protein Interactions. Structure, 12:495-502, 2004.
  • Wilkinson T and Chen Y. Analysis of Soluble Sugar Permease Domains by Solution NMR. Methods in Molecular Biology, 227:321-34, 2003.
  • Kim S, Zhu L, Yu B and Chen Y. Resonance Assignments of the ARID domain in Human SWI1. J Biomol NMR 27:277-8, 2003.
  • Hu W, Zhang Z, and Chen Y. A High Sensitivity 3D Experiment for Measuring C-H Residual Dipolar Coupling Constants. J. Magn. Reson. 165:248-52, 2003.
  • Zhang Z, Zhu L, Lin D, Chen F, Chen DJ, Chen Y. The Three-dimensional Structure of the C-terminal DNA-binding Domain of Human Ku70. J Biol Chem. 276, 38231-38236, 2001.
  • Zhu L, Hu J, Lin D, Whitson R, Itakura K, Chen Y. Dynamics of the Mrf-2 DNA-Binding Domain Free and in Complex with DNA. Biochemistry 40, 9142-50, 2001.
  • Wilkinson TA, Botuyan MV, Rossi JJ, and Chen Y. Arginine Sidechain Dynamics in the HIV-1 Rev-RRE Complex. J. Mol. Biol. 303, 515-529, 2000.
  • Yuan Y-C, Whitson RH, Liu Q, Itakura K, and Chen Y. A Novel DNA-Binding Motif Shares Structural Homology to DNA Replication and Repair Nucleases and Polymerases. Nature Structural Biology 5: 15-20, 1998.
  • Yuan Y-C, Whitson RH, Itakura K, and Chen Y. Resonance Assignments of the Mrf-2 DNA-Binding Domain. J. Biomol. NMR 11: 459-460, 1998.
  • Chen Y, Case DA, Fairbrother WJ, Reizer J, Saier MH, Jr., and Wright PE. High resolution NMR structure of the Bacillus subtilis glucose permease IIA domain, PROTEINS: Structure, Function and Genetics 31: 258-270, 1998.
  • Waring AJ, Chen Y, Faull KF, Stevens R, Sherman MA, and Fluharty AL. Porcine cerebroside sulfate activator (Saposin B) Secondary Structure: CD, FTIR and NMR studies, Molecular Genetics & Metabolism 63: 14-25, 1998.
  • Botuyan MVE, Momand J, and Chen Y. Solution structure of a peptide representing an essential region of the P53 transactivation domain, Folding and Design 2: 331-342, 1997.
  • Marsden I, Chen Y, Jin C, and Liao X. Evidence that the DNA binding specificity of winged helix proteins is mediated by a structural change in the amino acid sequence adjacent to the principal DNA binding helix, Biochemistry 36: 13248-13255, 1997.
  • Sherman MA, Chen Y, and Mas MT. An engineered amino-terminal domain of yeast phosphoglycerate kinase with native-like structure, Protein Science 6: 882-891, 1997.
  • Chen Y, Suri A, Kominos D, Naylor AM, Pitzenberger SM, Levy RM, and Baum J. Three dimensional structure of echistatin and dynamics of the active site, J. of Biomol. NMR 4: 307-324, 1994.
  • Chen Y, Fairbrother WJ, and Wright PE. Three-dimensional structures of the central regulatory proteins of the bacterial phophotransferase system, HPr and IIAglc, J. of Cellular Biochemistry 51: 75-82, 1993.
  • Chen Y., Reizer J, Saier MH, Jr., Fairbrother WJ, and Wright PE. Mapping of the binding interfaces of the proteins of the bacterial phosphotransferase system, HPr and IIAglc, Biochemistry 32: 32-37, 1993.
  • Chen Y, Pitzenberger SM, Garsky VM, Lumma PK, Sanyal G, and Baum J. Proton assignments and secondary structure of the snake venom peptide echistatin, Biochemistry 30: 11635-11636, 1991.

Locations

  • City of Hope - Main Campus (Duarte)
    1500 East Duarte Road
    Duarte, CA 91010