City of Hope’s Analytical Cytometry Core (ACC) Facility provides researchers with high-quality flow cytometry instrumentation, as well as expertise in analyzing and/or sorting sample populations of interest via interpretation of their physical, fluorescent and/or light-scattering properties. The facilities and their services are available to both City of Hope and non-
City of Hope researchers.
The ACC Facility has two components: the Flow Cytometry Facility (FCF) and the Molecular Imaging Facility (MIF).
As an added convenience, the ACC supports several off-line workstations throughout the campus where investigators can analyze their ACC-derived data. FCF software includes Flowjo (only on some workstations), Modfit (only on some workstations) and Summit. MIF software includes ImageQuant, PDQuest (Bio-Rad) or Odyssey.
The Flow Cytometry Facility has three Cell sorters (MoFlo, Aria III, and Aria SORP) and 5 analyzers (CyAn, Gallios, Fortessa, C6, and Guava). All the analyzers are available for unassisted end user runs.
Molecular Imaging Facility
This facility contains instruments utilizing lasers to scan gels, membranes (e.g., nylon, nitrocellulose), microtiter plates or thin-layer chromatography plates. The molecules of interest (DNA, RNA or proteins) are labeled with radioactive or fluorescent tags. There are two types of instruments available:
- The Typhoon 9410s (General Electric) has four excitation sources (457 nm, 488 nm, 532 nm and 632.8 nm). The Typhoon can also be used to acquire radioactive labeled data with a special phosphor screen, which is used to capture quantitative information that is then scanned on the Typhoon. This facility contains two Typhoon scanners.
- Odyssey Infrared Imager (Li-Cor) has two excitation sources (680 nm and 780 nm).
Last Updated: 01/04/12