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Supported by City of Hope's NCI-funded Cancer Center Support Grant (CCSG)

 CyAn

The CyAn™ ADP 9 Color has three excitation sources: 405nm, 488nm & 635nm. This instrument has 11 parameter capabilities (two scatter and nine fluorescent detectors). All parameters can be collected in linear peak height, linear area, and/or log scales. It is capable of acquisition rates up to 50,000 events per second. This cytometer is available for trained users to operate, or user samples may be analyzed by the core at an additional cost.

 

Photo of CyAnCyAn™ (Flow Cell)
 

 

Photo of CyAn ADPCyAn™ ADP
(Advanced Digital Processing)



Quality Control
Standard fluorescent beads are run once a week to ensure proper laser alignment for stability and reproducibility.

Supplies
Investigators must supply their own reagents.  We have an extensive library of vendors to facilitate the search and purchase of reagents.  We do have a variety of fluorochrome labeled secondary anti-mouse IgG antibodies, which we can supply on a trial basis if the investigator would be interested in trying multi-color staining.  Users that are trained to run their own samples on the analyzers (CyAn™ or FACScalibur) will need to provide their own tubes.

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