The MoFlo™ MLS has a four-laser optical bench configuration.  The four lasers (one HeNe, two argon gas, and one krypton gas) provide fluorescent excitations from 351-752.5 nm.  The instrument is built around dedicated electronics with control of acquisition, sorting and analysis directly from a computer with dual CPU and dual monitors.  This instrument has 10 separate photomultiplier tubes (PMTs) plus a forward scatter detector.  Peak height, integrated and logarithmic data can be collected from each of the PMTs.  A digital signal processor (DSP) computed-parameter board allows for up to 32 parameters (including pulse width on the trigger event and time) to be stored in listmode for a sample.  DSP also allows for 8x8 inter-laser compensation of fluorescence parameters for sorting.  The facility normally sorts with a 100 micron nozzle at 30 PSI.  Up to four sort decisions can be performed simultaneously.  Along with bulk sorting, cell deposition into wells as single cells or multiple cells is also a possibility.  The instrument can accommodate plates that contain from 6 to 384 wells.

MoFlo: 4-way sorting streams		MoFlo: Cytomation sorting unit
MoFlo MLS (Multi-Laser System)

Quality Control
Bead fluorescent standards are used to align the solid state HeNe, two argon gas lasers, and one krypton gas laser.  Once a month, mean equivalent fluorescence standards (FITC, PE) are used to check the linearity and sensitivity of the instrument.

Supplies Needed
Investigators must supply their own reagents.  We have an extensive library of vendors to facilitate the search and purchase of reagents.  We do have a variety of fluorochrome labeled secondary anti-mouse IgG antibodies, which we can supply on a trial basis if the investigator is interested in trying multi-color staining.  We provide the tubes necessary to sort user samples on the MoFlo™.  The investigator should supply the facility with the plates or tubes with appropriate media or collection solution to collect the sorted cells.