The Typhoon™ 9410 equipment is available to scan phosphor screens, which have been exposed to radioactive sources on gels/blots/dishes/trays/glass slides. The Analytical Cytometry Core has a large format (35x40 cm) phosphor screen and several small (20x25 cm) format screens available for proof of methodology. Screen erasers are located at the core workstations or nearby. This allows linear quantification of data (over five orders of magnitude) and produces digital images, which are directly manipulatable for analysis and publication. Exposure times are usually 20-25% of those required on film. Radioisotope sources have included P32, S35, C14, and I125. There are also special screens available from Amersham Biosciences for tritium detection.
The Typhoon™ 9410 also has the capability to detect fluorescent labels on gels, blots, dishes, trays, and glass slides. This instrument has four excitation sources: 457nm, 488nm, 532nm, and 633nm that allows for use of a wide range of fluorescent dyes. There are also selectable emission filters (520BP40, 555BP20, 580BP30, 610BP30, 670BP30, 526SP, and 560LP), which allow for collection of specific and multiple signals. This methodology allows for linear quantification of the data (over three to four decades of magnitude) and produces digital images, which are directly manipulatable for analysis and publication. The matrix of interest can be scanned at various resolutions depending on experimental design. For radioactivity, the range in resolution is from 25u to 1000u and for fluorescence, the range is from 10u-1000u. At 10u the scanner is capable of handling microarray slides.
Users are generally responsible for running their own controls.
Cleaning solution and Kimwipes® are provided for cleaning the platen on the scanner. All other reagents, solutions, buffers, and supplies must be provided by the user. Several phosphor screens are provided by the core for radioisotope scans, but users are encouraged to buy their own screens.
