The DNA Sequencing/Solexa Core is a shared resource facility of Beckman Research Institute of City of Hope. The laboratory is located in the Rosenkranz Building and occupies 800 sq ft of lab space. The core is equipped with state-of-the-art Illumina Genome Analyzer II systems, including a Cluster station and a Paired-end module.

The core also has a state-of-the-art Hitachi AB model 3730 48-capillary DNA Analyzer with the capacity to analyze 4,000 to 8,000 samples per week.

Services:

Conventional DNA sequencing by ABI 3730
We provide DNA sequencing, microsatellite, fragment-sizing, and SNP analysis. Several chemistries for sequencing difficult samples are available. Technical help, troubleshooting, primer design, editing and contig construction are also provided.

Massively parallel sequencing by Solexa
We provide consulting on experiment design, library preparation and sequencing for following services:

• DNA sequencing -- Leverage single-read, long- and short-insert paired-end reads for de novo sequencing, whole-genome sequencing, targeted re-sequencing, structural variation, and copy number analysis.
• RNA-sequencing -- Characterize splice variants, coding SNPs, and relative expression of transcripts in one experiment; profile rare and discover RNA
• miRNA/smRNA -- miRNA or smRNA profiling and discovering.
• ChIP-sequencing -- Study DNA-protein or RNA-protein interactions across the entire genome
• Methyl-sequencing -- Comprehensive study of the epigenome
• Other genome-scale analysis -- The Core also provides other applicable services for special applications required by researchers.

We provide help from the inception of a research idea, to the experimental design, and analysis for publication of results.