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 Electron Microscopy Core

The Electron Microscopy Core is available to investigators wishing to define ultrastructural details in their experimental systems. The EM Core assists with all aspects of studies brought to the lab including training, experimental design and interpretation of results.

A variety of preparation methods are available for transmission and scanning electron microscopy including ultra-thin sections of conventionally fixed samples, immunogold labeling, plunge-freezing cryo-EM and tomography.
 
Services:

  • Cryo-EM
  • Electron Tomography
  • Conventional EM
  • Negative Staining
  • High Pressure Freezing/Freeze Substitution
  • Immmunogold Labeling
  • Scanning Electron Microscopy
  • Single Particle Analysis
  • Single 3-D Reconstruction

 EM Micrograph Gallery

  • Birbeck bodies by TEM
  • Nerve enervation by SEM

 Scheduling

To discuss possible applications and use of the facility, contact Dr. Mariana Tihova at 626 301-8265 or Dr. Marcia Miller at 626 301-8264.  Assistance with experimental design and training will be provided as desired.

Turnaround time for TEM depends on method of sample preparation; 5-7 days for typical resin-embedded samples and 1 hr for negative staining.  SEM samples typically take 1 - 3 days.

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