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Cryo-EM of samples frozen in vitreous ice.

Tomography and assistance with three-dimensional reconstructions.

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To discuss possible applications and use of the facility, contact Dr. Zhuo Li at 626 301-8265 or Dr. Marcia Miller at 626 301-8264. Assistance with experimental design and training will be provided as desired.

Typical turnaround times for specimen preparation:

  • 1-2 hours for negative staining
  • 1-3 days for SEM
  • 5-7 days for resin-embedded specimens
  • 10-12 days for immunolabeling of specimens which must be first high pressure frozen, fixed, embedded in Lowicryl by freeze substitution and sectioned
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