Capacity for single nucleotide polymorphism (SNP) genotyping can run from the small-scale analysis of a few hundred SNPs to large projects with over 10,000 SNPs. We provide options for investigators that take into account both the number of patient samples to be genotyped and the number of desired SNPs to be analyzed. All SNP genotyping projects will employ the Sequenom MassARRAY iPLEX Gold system.
Highlights include:
- Multiplex PCR amplification and robust single-base primer extension biochemistry with the sensitivity and accuracy of MALDI-TOF MS detection.
- Assays can be multiplexed up to 30 SNPs in one individual reaction allowing for high-throughput levels and reduced chemistry cost and dependent upon assay design.
- Oligonucleotides are unlabeled and of standard quality.
Applications for iPLEX Genotyping Include:
- Mutation Detection
- Copy number variants analysis (CNV)
- Insertion/Deletions
- Disease association studies (follow up to GWAS)
- Loss of heterozygosity analysis
- Allelotyping of pooled samples
A brief description of the protocol can be found in the SNP Genotyping Brochure.
