The Functional Genomics Core (FGC), with scientific director, Charles Wang, M.D., Ph.D., M.P.H., is one of the National Cancer Institute (NCI) cancer center supported facilities under Beckman Research Institute (BRI) of City of Hope. The overall goal of the FGC is to provide state-of-the-art instruments, scientific and technical advice in genomics to City of Hope and non-City of Hope investigators. The FGC consists of three core components including microarray, genetic markers and RNAi services. The core is equipped with state-of-the-art instruments such as Affymetrix GeneChip Analysis System, Agilent scanner/microarray system, Roche NimbleGen MS200 microarray scanner/system and Illumina HiScanSQ system, a next-generation microarray platform integrated with next-generation sequencing capability using the same scanner, delivering unprecedented flexibility for genomic research. The core also has a SciGene Little Dipper Processor, a robotic instrument that automates washing and drying of microarray slides in an ozone-free environment. In addition, the core has an Amaxa Nucleofector device II, which allows efficient transfection of RNA or DNA into primary cells and difficult-to-transfect cell lines, and a next-generation ABI Taqman Real-time PCR system, ViiA 7, which is an excellent technology for microarray validation. Partially funded by the NCI cancer center grant, the FGC provides comprehensive support in genomic research including transcriptomic and miRNA profiling, ChIP-Chip, DNA methylation, genome-wide and customer genotyping, SNP/CNV, aCGH, deep sequencing, RNAi and qRT-PCR. The FGC has recently set up numerous new genomic technologies and assays including microarray-coupled, genome-wide gene expression profiling using difficult clinical FFPE samples, microfluidic chip- and microarray-coupled single-cell genome-wide gene expression profiling, NimbleGen array-based CHARM (comprehensive high-throughput arrays for relative methylation), Affymetrix DMET and genome-wide human SNP 6.0 array (1.8M markers) genotyping, and Illumina Infinium HumanMethylation450 BeadChip which interrogates more than 450,000 methylation sites across the whole human genome at single-nucleotide resolution.