The RNAi laboratory provides siRNA design, transfection and validation services to City of Hope and Beckman Research Institute scientific investigators.

One of two methods is employed to transfect siRNA duplexes into target cells: (1) cationic lipids or (2) electroporation. The RNAi Service has an Amaxa Nucleofector Device for efficient transfection of primary and difficult-to-transfect cell lines.

The siRNA-induced knock-down of gene expression is monitored by quantitative RT-PCR using the Bio-Rad iQ5 Multicolor Real-Time PCR Detection System, capable of analyzing up to 5 targets in multiplexed PCR assays.

The RNAi Service is located in the Flower Building.