Conventional Microscopy Modes

• Bright Field
• Nomarski DIC
• Fluorescence

Laser Scanning Modes

• Argon Laser 458, 488, 514 nm
• HeNe 1 Laser 543 nm
• HeNe 2 Laser 633 nm
• Tunable IR Ti-Sapphire Pulse Laser 730-950nm (2-Photon Excitation)
• 3 PMT detectors with individually controlled pinholes
• 2 Non-Descanned Detectors
• META polychromic 32 channel Spectral Detector
• Transmitted light mode

Imaging Features

• Motorized Stage XY movement for tiling, Z for optical sectioning
• Single or Multi-track image collection
• 8 or 12 bit pixel depth
• Lambda stack scanning
• Linear Unmixing of spectral scans
• Emission fingerprinting
• Multiple ROI scanning
• 3D projection from image stack, depth code, stereo pairs, red and green stereo images
• 128, 256, 512, 1024, and 2048 pixel frame size
• Ratio imaging
• Multi-Time Macro for time lapse imaging
• Physiology Macro
• Continuously adjustable digital zoom
• Detector rotation
• Monitor Diode Detector reduces laser noise
• Overlay of DIC or phase on fluorescent image
• LSM 4.2 on Windows XP Platform
• Other:  an accessory incubator that controls temperature and CO2 levels during time lapse imaging of growing cells and tissue

Configuration

• View a diagram of the LSM510 inverted configuration

Dyes

• View the fluorescent dyes available for confocal microscopes

Confocal Microscope Images

• View confocal images shot with the microscopes available in the core facility

Objectives

• View a list of available objectives for confocal microscopy

Last updated: 7/1/08