Fully automated research class microscope complete with linear encoded stage capable of Phase Contrast (5X only), DIC, BF, and Fluorescence Illumination utilizing the Exfo Xcite.

Top of the line M27 thread objectives include:

• 5X/0.16NA Plan-NeoFluar Phase
• 10X/0.5NA Fluar DIC
• 25X/0.8NA LCI PlanApo Multi Immersion DIC, Correction Collar, LD (working distance 570um)
• 63X/1.3NA Plan-NeoFluar Multi Immersion DIC, Correction Collar (working distance 170um)

Microscope also equipped with Optovar that can increase magnification by 1.6X  (i.e., 25X * 1.6X = 40X).

Filter Cube Spectra:
#49 Ex; G369nm, Beam Splitter; FT395, Emision; 445/50 (DAPI)
#38 Ex; BP470/40, Beam Splitter; FT495, Emission; 525/50 (eGFP)
#43 Ex; BP545/25, Beam Splitter; FT570, Emission; 605/70 (DS Red)
#50 Ex; BP640/30, Beam Splitter; FT660, Emission; 690/50 (Far Red)

Hamamatsu EMCCD C9100-13 Monochrome Camera
Super sensitive, and super fast, camera for high speed fluorescence acquisition.
Back-Thinned 512x512 pixel chip, 16um x 16um pixels, 31.9fps to 404fps* acquisition speed (*with gain, binning, and sub-array), Quantum Efficiency ~95%.

DELL OptiPlex 755 Computer
Intel 2 Duo Processor (3GHz), 256MB ATI Radeon 2400XT video card, Dual DELL 19” Flat Panel Monitors, 4GB RAM.

Zeiss AxioVision software version 4.8
Capable of automated imaging for; Time-Lapse, Z-stacks, Tiling, Multiple Positions, and Multi-Colors.

Pecon / Zeiss Incubation System 
Heated stage insert, large heated enclosure, CO2 control. Can be automated via TFT or in AxioVision. 


Last updated: 9/23/08