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Supported by City of Hope's NCI-funded Cancer Center Support Grant (CCSG)

 Mass Spectrometry and Proteomics Core

The Mass Spectrometry and Proteomics Core provides high-quality mass analysis of biomolecules – proteins, glycoproteins, peptides, oligonucleotides, nucleic acids and small-molecule metabolites – for a variety of biomedical investigations. The facility offers supporting techniques as well as scientific consultation. Operations of the core are assisted by the National Cancer Institute-funded Cancer Center Support Grant.

 Notable capabilities of our facility include:
  • Purified proteins and protein complexes (isolated by affinity chromatography) can be rapidly identified by fully-automated liquid chromatography-tandem mass spectrometry (LC/MS/MS) analyses combined with our protein database search capabilities.
  • Relative protein expression levels can be determined for lysates of whole cells or subcellular organelles.
  • Absolute levels of specific peptide components in complex biological samples can be determined (with appropriate
    stable isotope-labeled standards) via multidimensional separations and MS analysis.
  • High-resolution mass spectrometry using matrix-assisted laser desorption ionization (MALDI) or electrospray ionization (ESI) methods provide highly accurate determination of small molecule elemental composition.
  • Qualitative and quantitative analysis of small molecules by LCMS/MS.
  • High Resolution MS and MSn    

Last updated:  02/02/2009

 Services

  • MALDI Mass Spectrometry
  • ESI Mass Spectrometry (nanospray)
  • ESI MSn Analysis (nanospray)
  • LC/MS Analysis
  • LC/MS/MS Analysis of small molecules, metabolites, lipids, peptides, including quantitation.
  • Multidimensional peptide and protein separations by LC and off-gel electrophoresis
  • In-gel Reduction, Alkylation, and Digestion of Coomassie Blue, Silver, and Fluorescent Dye stained gels
  • Solution Phase Reduction and Alkylation
  • Solution Phase Digestion
  • Sequest, GPM, and Spectrum Mill Database Searches for Protein Identification

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 Contact Us

E-mail us for pricing or more information about Mass Spectrometry or to schedule an appointment for services or equipment.

Faculty & Staff

Location:
City of Hope and Beckman Research Institute
1500 East Duarte Road
Duarte, CA  91010-3000

Hilton Building
Room H134

Phone:  626-256-HOPE (4673), ext. 62601
Fax:  626-301-8186

 

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