The Mass Spectrometry and Proteomics Core provides high-quality mass analysis of biomolecules – proteins, glycoproteins, peptides, oligonucleotides, nucleic acids, lipids and small-molecule metabolites – for a variety of biomedical investigations. The facility offers supporting techniques as well as scientific consultation. Operations of the core are assisted by the National Cancer Institute-funded Cancer Center Support Grant.

Notable capabilities of our facility include:

• Purified proteins and protein complexes (isolated by affinity chromatography) can be rapidly identified by fully-automated liquid chromatography-tandem mass spectrometry (LC/MS/MS) analyses combined with our protein database search capabilities.
• Relative protein expression levels can be determined for lysates of whole cells or subcellular organelles as well as paraffin embedded tissue sections.
• Absolute levels of specific peptide components in complex biological samples can be determined (with appropriate stable isotope-labeled standards) via multidimensional separations and MS analysis.
• High-resolution mass spectrometry using electrospray ionization (ESI) provides highly accurate determination of small molecule elemental composition.
• Qualitative and quantitative analysis of small molecules by LCMS/MS.  
• High Resolution MS and MSn 

Last updated:  09/20/2010

• ESI Mass Spectrometry (nanospray)
• ESI MSn analysis (nanospray)
• LC/MS analysis
• LC/MS/MS analysis and quantitation of small molecules, metabolites, lipids, peptides, nucleosides.
• Multidimensional peptide and protein separations by 2D LC and off-gel electrophoresis/LC. on mobility will be added in the near future as an extra dimension.
• In-gel Reduction, Alkylation, and Digestion of Coomassie Blue, Silver, and Fluorescent Dye stained gels
• Proteomic analysis of paraffin embedded tissue sections
• Solution Phase Reduction and Alkylation
• Solution Phase Digestion
• Sequest, Global Proteome Machine (GPM), Spectrum Mill and Protein Lynx Global Server Database Searches for Protein Identification
• Label free quantitation of proteins in samples and expression analysis.

E-mail us for pricing or more information about Mass Spectrometry or to schedule an appointment for services or equipment. Please see the Fee Schedule for pricing for various analyses or shared instrument usage. A completed submission form must accompany all samples submitted for analysis. Please download the submission form from this website.
Faculty & Staff
Location:
City of Hope and Beckman Research Institute
1500 East Duarte Road
Duarte, CA  91010-3000
Hilton Building
Room H134
Phone:  626-256-HOPE (4673), ext. 62601
Fax:  626-301-818