The Mass Spectrometry and Proteomics Core provides high-quality mass analysis of biomolecules – proteins, glycoproteins, peptides, oligonucleotides, nucleic acids and small-molecule metabolites – for a variety of biomedical investigations. The facility offers supporting techniques as well as scientific consultation. Operations of the core are assisted by the National Cancer Institute-funded Cancer Center Support Grant.

 Notable capabilities of our facility include:

• Purified proteins and protein complexes (isolated by affinity chromatography) can be rapidly identified by fully-automated liquid chromatography-tandem mass spectrometry (LC/MS/MS) analyses combined with our protein database search capabilities.
• Relative protein expression levels can be determined for lysates of whole cells or subcellular organelles.
• Absolute levels of specific peptide components in complex biological samples can be determined (with appropriate
  stable isotope-labeled standards) via multidimensional separations and MS analysis.
• High-resolution mass spectrometry using matrix-assisted laser desorption ionization (MALDI) or electrospray ionization (ESI) methods provide highly accurate determination of small molecule elemental composition.
• Qualitative and quantitative analysis of small molecules by LCMS/MS.
• High Resolution MS and MSⁿ    

Last updated:  02/02/2009

• MALDI Mass Spectrometry
• ESI Mass Spectrometry (nanospray)
• ESI MSn Analysis (nanospray)
• LC/MS Analysis
• LC/MS/MS Analysis of small molecules, metabolites, lipids, peptides, including quantitation.
• Multidimensional peptide and protein separations by LC and off-gel electrophoresis
• In-gel Reduction, Alkylation, and Digestion of Coomassie Blue, Silver, and Fluorescent Dye stained gels
• Solution Phase Reduction and Alkylation
• Solution Phase Digestion
• Sequest, GPM, and Spectrum Mill Database Searches for Protein Identification

E-mail us for pricing or more information about Mass Spectrometry or to schedule an appointment for services or equipment.

Faculty & Staff

Location:
City of Hope and Beckman Research Institute
1500 East Duarte Road
Duarte, CA  91010-3000

Hilton Building
Room H134

Phone:  626-256-HOPE (4673), ext. 62601
Fax:  626-301-8186