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Supported by City of Hope's NCI-funded Cancer Center Support Grant (CCSG)

 Abstract for Grants
The City of Hope Cytogenetics Core laboratory provides classic and molecular cytogenetics [fluorescence in-situ hybridization (FISH)] services to the City of Hope and Beckman Research Institute scientific investigators.  The Cytogenetics Core laboratory equipment, including high-quality fluorescence and light photomicroscopes, three computerized imaging systems, tissue culture facilities, and FISH instrumentation for probe labeling and hybridization, is available to City of Hope and Beckman Research Institute researchers on an appointment basis.  The core laboratory also provides training, cell line tumor banking, and consultation services for applied molecular cytogenetic testing.

Images

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Mouse 20-color karyotypingNormal Mouse 20-color karyogram (SKY)

  

Standard FISH mapping and 24-color karyotyping

Standard FISH mapping and 24-color karyotyping.  A) GTG banding, B) FISH, C) GTG, FISH, and SKY sequential analysis showing translocation of chr. 2 and chr. 13

FISH MappingMapping of a 200kb insert on chromosome 2

12 cent APAF inverse DAPI FISHInverse DAPI image showing fluorescence in-situ hybridization of homebrew probe on 12 centromere and 12q24.22 (APAF1).  The 12 centromic probe is labeled with FITC, while the APAF1 probe is labeled with rhodamine.

HDLM-2 karySKY metaphase of a human Hodgkin lymphoma cell line (HDLM-2), showing a large number of rearrangements


CD10 target FISHTargeted scanning with CD10 monoclonal antibody

HDLM-2 kary24-color karyotyping (SKY) of HDLM-2.  The karyogram shows a large number of rearrangements involving all chromosomes except chr. 10

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1500 East Duarte Road
Duarte, California 91010
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