We offer ultra high throughput DNA sequencing services on the Illumina HiSeq 2000 platform. Using a massively parallel sequencing approach, the Illumina HiSeq 2000 can generate up to 30GB of data in a single lane or up to 240 GB of data in a single run. The system uses Solexa sequencing technology and novel reversible terminator chemistry, optimized to achieve unprecedented levels of cost effectiveness and throughput.
Learn more at Illumina's website >>
The 454 Genome Sequencer FLX Titanium platform generates about 1 million reads with an average length of 300-500bp in a typical run. It is an excellent platform for de novo sequencing and for projects that require longer read lengths. The GS FLX Titanium accommodates shotgun, paired-end, and amplicon sequencing runs.
Learn more at the Roche 454 website >>
The following technologies have been developed for this platform:
DNA-seq:de novosequencing or resequencing
ChIP-seq: DNA-protein interactions across the entire genome
Methyl-seq: methylation profiling
RNA-seq:mRNA expression, splicing, mutations/cSNP
smRNA-seq: small RNA/miRNA discovery, profiling
Structure variant detection: deletions, duplications, insertion, inversion and rearrangements
Multiplexed Sample Sequencing with barcodes