Scientific Accomplishments
Since its inception, the original DNA/RNA and Peptide Synthesis Core has had significant impact on the successful execution and completion of a number of research projects. This core has a long history of scientific achievement and innovation. Dr. Kaplan and co-workers were among the early developers and users of automated DNA and peptide synthesis, having designed and built early prototypes upon which current commercial instruments are based.
The biopolymer synthesislaboratory provides a variety of custom oligonucleotides to the research community of City of Hope Comprehensive Cancer Center. The DNA and RNA may be synthesized with many reagents containing modified bases and sugars. In addition, a variety of labels and linkers can be incorporated into the oligomer. Some of the reagents are commercially available but some have to be synthesized in our laboratory. In addition, the facility has produced synthetic reagents that allows for the introduction of cis and 6-4 TT photodimers into an oligonucleotide. The biopolymer lab is capable of synthesizing peptides in excess of 100 amino acids. The laboratory has successfully synthesized peptides with phospho-Thr and -Ser, as well as peptides with biotin and acroyl groups attached to the amino terminus.
The facility has recently added the production of fluorescently-labeled peptides to its repertoire and has pioneered the synthesis of chimeric DNA/RNA/DNA molecules for use as anti-sense drugs. As a significant portion of these technologies is developed “in-house” to meet a specific research demand, they have been successfully applied to a myriad of City of Hope Comprehensive Cancer Center projects.