A National Cancer Institute-designated Comprehensive Cancer Center

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Widefield Light Microscopes

Widefield Light Microscopes
For more information on one of the microscopes listed below, please click on the appropriate tab above.

  • Olympus IX81Automated Inverted for culture dish specimens. Color, fluorescence, and mono
  • Olympus BX50 Upright for fluorescence, mono camera
  • Olympus Vanox Upright for brightfield, color, and fluorescence
  • Leica Macrofluar Z-16 Fluorescent Macroscope, mono camera
  • Zeiss AxioVert 200 Inverted Microscope, mono camera
  • Zeiss Axio Observer Z1 Inverted Microscope, live-cell imaging station, color and mono cameras


Scheduling Equipment
 

Olympus IX81 Automated Inverted



Observation Methods
 
  • Bright Field
  • Phase Contrast
  • Differential Interference Contrast (DIC)
  • Fluorescence
     

Digital Imaging

  • Q-Imaging Retiga 2000R high-resolution cooled CCD (color and mono imaging modes)
     

Computer and Software

  • PC workstation with general office software and network connections.
  • Q-Imaging camera captures large frame of 1600 x 1200 pixels using Image Pro Plus v7.0.
  • Olympus IX2-BSW v.1.3 microscope control software.
  • Image Pro Plus v7.0 high-end image acquisition processing and analysis software.
  • ScopePro/StagePro microscope automation software: automation of most microscope controls and observation methods, stage XY and Z positions, optical Z sections, time lapse combined time controls and Z sectioning.
     

Other Features

  • A variety of stage inserts to hold microscope slides, multi-well culture plates, round culture plates and flasks
     

Digital Light Microscope Images

  • Click on tab above to view examples of images shot with the available light microscopes.
     

Filter Cubes

  • Click on tab above to view examples of the filter cubes available.
     

Objectives

  • Click on tab above to view a list of available objectives.
     

Last updated: 2013

 

Olympus BX50 Upright Microscope

Observation Methods

  • Brightfield
  • Phase Contrast
  • Nomarski Differential Interference Contrast
  • Fluorescence
     

Digital Imaging

  • Hammamatsu Orca high speed / high resolution digital camera
     

Computer and Software

  • PC workstation with general office software and network connections
  • ImagePro Plus high-end image acquisition, processing, and analysis software
     

Digital Light Microscope Images

  • Click on tab above to view examples of images shot with the available light microscopes.
     

Filter Cubes

  • Click on tab above to view examples of the filter cubes available.
     

Objectives

  • Click on tab above to view a list of available objectives.

     

Last updated: 2013

Olympus AX70 Automated Upright

 


Observation Methods

  • Bright Field
  • Nomarski DIC
  • Fluorescence
  • Polarized Light
     

Digital Imaging

  • Retiga EXi
  • 1392 x 1040 Pixels
  • For low light
  • Fluorescence and brightfield imaging (mono and color modes)
     

Computer and Software

  • PC workstation with general office software
  • ImagePro Plus, high-end image acquisition and analysis software
  • ScopePro/StagePro microscope automation
  • Software: automation of most microscope controls and observation methods. Stage XY and Z positions optical Z sections, time lapse, combined time lapse z sectioning
     

Digital Light Microscope Images

  • Click on tab above to view examples of images shot with the available light microscopes.
     

Filter Cubes

  • Click on tab above to view examples of the filter cubes available.
     

Objectives

  • Click on tab above to view a list of available objectives.

     

Last updated: 2013

 

Leica Z16 Macroscope

  • Objectives: 0.32xAchro,0.5xApo,1xApo, 2xApo
  • Zoom range; 0.57x to 9.1x
  • Magnification: 1xApo; 7.1x to 115x (2xApo total mag 230x)
  • KL1500 Cold Light Source with double goosenecks
  • EL6000 Fluorescent Illumination (Exfo Xcite)
     

Filter Cubes

  • A4 for UV, BP 470/40
  • L5 for GFP, BP 527/30
  • RFP, BP 605/75
  • TX2 Texas Red, 645/75
     

Imaging Features

  • DVC Digital CCD camera
  • 1392x1040 pixel array
  • DVC Cam Software
     

Last updated: 2013

Zeiss AxioVert 200 Inverted Microscope



Observation Methods
  • Brightfield
  • Phase Contrast
  • Fluorescence (Exfo Xcite 120)
 

Digital Imaging
  • Zeiss Mr3 camera (mono, 1388x1040 pixel array)
 

Computer / Software
  • Dell Optiplex 790, Intel i5, 4 GB RAM, 24" Dell Monitor
  • Windows XP SP3
  • Zeiss AxioVision 4.8.2

Objectives
  • 5x/0.12NA A-Plan
  • 10x/0.3NA EC Plan-Neofluar
  • 20x/0.4NA LD Plan-Neofluar  Corr
  • 40x/0.6NA LD Achroplan Corr
  • 25x/0.8NA LD LCI Plan-Apo  Water Imm
 

This Zeiss Inverted microscope is ideal for imaging assays prepared in well plates in either brightfield, phase contrast or fluorescence imaging modes. The microscope has 5X, 10X, 20X and 40X LWD objectives. A Zeiss AxioCam CCD camera captures mono images with good sensitivity so that fluorescent imaging is easily accomplished. The system is operated by Zeiss AxioVision 4.8.2 software. The spectra of the filter cubes are designed to capture fluoresence from DAPI, GFP, Cy3 and CY5 (blue, green, red and far-red). Fluorescent excitation is supplied by an Exfo Excite with a 5 position neutral density filter for adjustable illumination.



Last updated: 2013

Zeiss Axio Observer Z1 Inverted



Observation Methods
Brightfield
Phase Contrast (5x only)
DIC
Fluorescence
 
 
Digital Imaging Cameras
Hamamatsu EMCCD C9100-13 Monochrome Camera
Very sensitive camera for high speed fluorescence acquisition.
Back-Thinned 512x512 pixel chip, 16um x 16um pixels, 31.9fps to 404fps* acquisition speed (*with gain, binning, and sub-array), Quantum Efficiency ~95%.
 
Zeiss MRc5 Digital Color Camera
Camera allows 36-bit color images to be acquired in high resolution due to small pixel size and large array (3.4umpixels, 2584x1936).
 
 
Available Objectives
  • 5X/0.16NA Plan-NeoFluar Phase
  • 10X/0.5NA Fluar DIC
  • 25X/0.8NA LCI PlanApo Multi Immersion DIC, Correction Collar, LD (working distance 570um)
  • 40X/0.95 PlanApo DIC
  • 63X/1.3NA Plan-NeoFluar Multi Immersion DIC, Correction Collar (working distance 170um)

Microscope also equipped with Optovar that can increase magnification by 1.6X (i.e., 25X * 1.6X = 40X).

 

Filter Cube Spectra:
#49 Ex; G369nm, Beam Splitter; FT395, Emision; 445/50 (DAPI)
#38 Ex; BP470/40, Beam Splitter; FT495, Emission; 525/50 (eGFP)
#43 Ex; BP545/25, Beam Splitter; FT570, Emission; 605/70 (DS Red)
#50 Ex; BP640/30, Beam Splitter; FT660, Emission; 690/50 (Far Red)

 

Computer and Software
DELL OptiPlex 755 Computer

Intel 2 Duo Processor (3GHz), 256MB ATI Radeon 2400XT video card, Dual DELL 19” Flat Panel Monitors, 4GB RAM.

Zeiss AxioVision software version 4.8
Capable of automated Multi Dimensional Acquisition imaging for; Time-Lapse, Z-stacks, Tiling, Multiple Positions, and Multi-Color.

 

Incubation
Pecon / Zeiss Incubation System
Large heated enclosure, heated stage insert, and CO2 control. Can be automated via TFT or in AxioVision.


Last updated: 2013

Filter Cubes

The following are filter cubes available in the Light Microscopy Digital Imaging Core.
 
IX81 Microscope
BX50 Microscope AX70 Microscope
U-M640 (Green Emission)
460-490 Excitation
U-MWB (Green Emission)
450-480 Excitation
U-MWIBA (Green Emission)
460-490 Excitation
     
U GFP Piston Bandpass
(Green Emission)
451-490 Excitation
U-MNG (Red Emission)
530-550 Excitation
U-MSWG (Red Emission)
480-550 Excitation
     
U-M660 WG (Red Emission)
510-550 Excitation
U-MNUA (Blue Emission)
360-385 Excitation
U-MNUA (Blue Emission)
360-385 Excitation
     
Texas Red (Red Emission)
543-588 Excitation
   
     
U-M620 WU2 (Blue Emission)
330-385 Excitation
   
 
Additional Cubes (Fit Olympus microscopes)
 
Yellow GFP (YFP)
Ex 500/20
Em 535/30
U-GFP Piston
(Green Emission)
Ex 470/40
Em 515/30
Q DOT Em 525/40

Q DOT Em 565/40
 
Cyan GFP (CFP)
Ex 436/20
Em 480/40
TEXAS RED
Ex 602/13
Em 631/23
Q DOT Em 605/20
Q DOT Em 655/40
TRITC (Very Red)
Ex 545/30
Em 596/60
   

 

Widefield Light Microscopy Images

DIC Golgi Staining Bright Field Silver Stain
HRP Immuno Staining DIC Striated Muscle
DIC Single Image DIC Single Image
Bright Field Single Image Extended Depth
 

Olympus Microscopes Objective Lens List

Microscope Magnification Name Numerical
Aperture
(NA)
Note
AX70 4x UPlanApo 0.16  
  10x UPlanApo 0.4  
  20x UPlanApo 0.7  
  40x UPlanFI 0.75  
  40x UPlanApo 1 Oil iris
  60x PlanApo 1.4 Oil
BX50 10x UPlanFI 0.3 Ph1
  20x UPlanFI 0.5 Ph1
  40x UPlanFI 0.75 Ph2
  60x PlanApo 1.4 Oil
IX81 10x UPlanApo 0.4 Ph1
  20x LCPlanFi 0.14 Ph1/CapP1.1
  40x LUCPlanFi 0.6 Ph2/Corr
  20x UPlanApo 0.7  
  40x LUCPlanFi 0.6 Corr
  60x UPlanApo 1.2 W/Corr
Vanox T 4x SPlan4PL 0.13  
  10x SPlan10PL 0.3  
  20x SPlan20PL 0.46  
  40x SPlan40PL 0.7  
  100x SPlan100PL 1.25 Oil
Other Available
Objectives
2x PlanApo 0.08 (Unknown)
  4x UPlanApo 1.16  
  4x SPlanApo 0.13 (Vanox)
  10x UPlanApo 0.4  
  20x UPlanApo 0.7 Ph2
  60x LCPlanFI 0.7 Ph2
  60x LUMPlanFI 0.9 W/Dip
  60x SPlanApo 1.4 Oil (Vanox)
  100x PlanAchormat 1.25 Oil

Last updated: 2013
City of Hope
Light Microscopy Digital Imaging Core
1500 East Duarte Road
Furth Building
Room F1113
Duarte, CA 91010
626-301-8870 phone
626-301-8908 fax
DIC Striated Muscle
Click here for more information on our Widefield Light Microscopes.
Developing fruit fly nervous system
Click here for more information on our Confocal Microscopes.
Research Shared Services

City of Hope embodies the spirit of scientific collaboration by sharing services and core facilities with colleagues here and around the world.
 

Beckman Research Institute of City of Hope is internationally  recognized for its innovative biomedical research.
City of Hope is one of only 41 Comprehensive Cancer Centers in the country, the highest designation awarded by the National Cancer Institute to institutions that lead the way in cancer research, treatment, prevention and professional education.
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