Equipment

The facility houses a Mosquito Crystallization Robot, a Formulatrix imaging robot (with Automated Crystal Screening at 4 ºC and 20 ºC), a Rigaku Micromax 007 with an R-AxisIV++, and an Oxford cryojet and relevant software and computational hardware for structure determination.
 
The facility also houses a Beckman XLI analytical ultracentrifuge and a GE Health Phast Gel system for the characterization of macromolecular properties.
 
Mosquito Crystallization Robot
The mosquito crystallization robot permits hanging drop, sitting drop, and batch methods in a 96 well format using minimal amounts of protein (~18 microL/trail). A number of commercial crystallization screens are available (Qiagen, Hampton Research, Jena).
   
Formulatrix Automated Visualization of Crystallization Trials
The core houses two Formulatrix imaging robots for visualization at 4 ºC and 20 ºC. Users can quickly browse images, score potential hits and follow crystal growth in time.
   
Rigaku X-ray Diffractometer
Micromax 007 HF, R-axis V++, and Oxford cryojets allow full structure determination.
 
 
Beckman XL-I Proteomelab
The Beckman Analytical ultracentrifuge affords accurate measurement of hydrodynamic properties, including the association constant of macromolecular complexes, as well as small molecule-macromolecular interactions.   It is also useful for formulation of biologics.
   
Waters NanoITC
Isothermal Titaction Calorimetry measures the transfers of thermal energy upon ligand binding.    Rom these measurements, one directly observes the enthalpy and measures the association constant of the interaction.  This in turn affords a measure of the entropy involved in an interaction.  These values provide important information about the nature of an interaction and are useful for drug design efforts.
   
Biacore T100 SPR
The GE Healthsciences Biacore provides kinetic and thermodynamic information for macromolecular interactions (protein-protein, DNA-protein, etc.), ligand-protein interactions, and fragments.
   
Jasco 815 Circular Dichroism
The Jasco 825 CD with fluorescence detection provides information vis-à-vis secondary structure.  Combined with a Peltier thermal control, this instrument is useful for determining melting temperatures and how point mutations affect protein stability.  In addition, the fluorescence detection can be used to determine the affinity protein-protein/protein-DNA interactions, how potential therapeutics affect such interactions, etc.
   
Sapidyne Kinexa 3200
The Sapidyne Kinexa determines binding constants and kinetics of an interaction by measuring the free ligand available (as opposed to SPR which measures the complex).  This allows the Kinexa to measure much tighter affinities (down to 10 femtomolar).