BD Bioscience Aria III:
The Aria III is a four laser system (405nm, 488nm, 561nm & 633nm) with a maximum of 15 fluorescent detectors. Place link for standard configuration:colleen let me know if you can upload and I’ll send to you. Samples can be loaded onto the sorter in a variety of tube sizes (microfuge tubes, 12X75mm or 15ml conical centrifuge tubes). The possible nozzle tip sizes available for sorting are 70 micron, 85 micron, and 100 micron. Bulk sorting of 1 to 4 populations per sample is possible. Collection tubes for 1 or 2 way sorting are 15ml, 12X75mm, or 1.5ml microfuge tubes. With 3 or 4 way sorting, either 12X75mm or 1.5ml microfuge collection tubes are utilized. Also, an Automatic Cell Deposition Unit (ACDU) is available for sorting onto slides and plates (6, 24, 48, 96 or 384-well plates). With the ACDU single cell or any user specified number of cells can be collected. The Aria III is equipped with temperature control for both the sort sample and the sort collection tubes. There is also an Aerosol Managemnet Option (AMO) to minimize aerosol release during cell sorting.
Cytometer Setup and Tracking (CS&T) beads are purchased from BD Bioscience and run on a daily basis to monitor system performance. Also Accudrop beads (BD Bioscience) are utilized to setup the sorting parameters of the system prior to cell sorting for a given day.
Investigators must supply their own reagents. All samples are filtered by core staff prior to placement on the cell sorter. We utilize 60micron nylon mesh that has been autoclaved for sterility. It is recommended that the user provides collection tubes with the appropriate media for their specific cell type. For plate sorting, the user must provide the appropriate plate type and collection media. It is also recommended that the user bring extra collection media.