Jyotsana Singhal, M.S.
- Staff Scientist, Department of Diabetes Complications & Metabolism
Jyotsana Singhal, M.S.
Jyotsana Singhal was born in Agra, India, where she received her education, B.S. in 1988 and M.S. in 1990, from Agra University, Agra, India, and moved to USA in 1992. She was trained as a Lab Technician (1992 – 1995) with Prof. Bogdan Nowicki at the University of Texas Medical Branch (UTMB) at Galveston, TX, where she was promoted to Research Assistant (1996 – 1999). She joined the University of Texas at Arlington (UTA), Arlington, TX in 1999, where she was Research Associate. After that, she joined the University of North Texas Health Science Center (UNTHSC), Fort Worth, TX as Senior Research Associate in 2007, and later on she joined Beckman Research Institute of the City of Hope, National Medical Center, Duarte, CA, as Staff Scientist in 2012.
Her current research interests are in multi-drug resistance in cancer. Her research objectives and expertise are in: 1) Stress signaling and oxidative stress, 2) Carcinogenesis and chemoprevention, 3) intracellular immuno-staining and multicolor flow-cytometry, and 4) Syngeneic and xenografts model. She has published more than 50 peer-reviewed research articles in these and related areas.
- 2012 to present - Staff scientist, Department of Diabetes Complications & Metabolism at City of Hope's Diabetes & Metabolism Research Institute, Duarte, CA
- 2007 to 2012 - Senior research associate, Department of Molecular Biology & Immunology, University of North Texas Health Science Center, Fort Worth, TX
- 1999 to 2007 - Research associate, Department of Chemistry and Biochemistry, University of Texas, Arlington, TX 1995 to 1999 - Research assistant, Department of Obstetrics-Gynecology, University of Texas Medical Branch, Galveston, TX
- Diabetes Complications & Metabolism
- 1990 - Agra University, Agra, India, M.S. in Organic Chemistry
More than 20 years of extensive, diverse and advanced expertise in molecular biology focused on testing anticancer drugs, lead compounds and combinations of drugs. Successfully accomplished completion of projects utilizing integrated multi-disciplinary technical experience in multiple laboratories in projects focused on carcinogenesis, drug-resistance and oxidative-stress. She has trained a number of under-grad students and doctoral candidates on experimental design, standardization and conduct in multiple molecular biology methods.
She is also experienced in design and conduct of ELISA assays, immuno-fluorescence staining, flow-cytometry, protein biochemistry, OCT- and paraffin-embedded tissue sections, histology, bacterial binding and invasion on cells, immunology, cell culture, drug-sensitivity assay, X-irradiation, carcinogenesis, animal experiments, gene-expression analysis, RNA sequencing, DNA methylation, and Seahorse XFe 24/96 analyzer. The Seahorse XFe 24/96 is revolutionary instrument for studies of cellular energy metabolism. This instrument measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of living cells, this can also measure respiration of isolated mitochondria.
- RLIP76 is a stress-protective or stress-responsive membrane associated non-ATP binding cassette (ABC) multi-specific transporter of chemotherapeutic drugs as well as of glutathione-electrophiles conjugates (GS-E) and involved in signaling pathways. “RLIP76 is a novel glutathione-conjugate and multi-drug transporter”.
- Our studies have revealed that inhibiting or depleting RLIP76 leads to regression of multiple types of cancers like melanoma, neuroblastoma, colon, kidney, pancreas, and lung cancers in human xenografts, which signifies the essential role of RLIP76 in different types of cancers.
- We have investigated the anticancer effects of flavonoids like 2´-hydroxyflavanone, vicenin, and didymin in chemoprevention studies on various cancers of kidney, prostate and neuroblastomas.
- Recently, we synthesized a novel compound, COH-SR4, [1,3-bis-(3, 5-dichloro-phenyl)urea], , a novel small molecule developed in house at City of Hope that selectively affects mitochondrial function and is very effective in both syngeneic and nude mouse models of melanoma.
Complete List of Published Work in MyBibliography: