Logistics

FAQs

What should be included with the specimen?
  • A completed Test Request Form (TRF) with complete billing information is required for all tests.
  • Clinical information (e.g. relevant clinic notes, pedigrees, other lab results) is recommended for optimal interpretation and genotype analysis for your patient.
  • If requesting testing for known familial mutations, a copy of the proband mutation report is recommended.
What are your prices?

For the current price list, please email us at [email protected] with your name, title and institution.

Are samples accepted on the weekend?

It is preferred that samples arrive at CMDL during the week, however we are capable of receiving samples at other times.
Exception: Cultured amniocytes/CVS submitted for prenatal testing must be received in the lab M/Tu/W.

Specimen Requirements

Tests for Heritable Genetic Disorders

Blood samples: 2 tubes with a total of volume of 6 ccs in EDTA (lavender top)  or ACD (yellow top) tubes. Keep at ambient temperature and ship by overnight courier. Samples must be received in our laboratory within 72 hours of draw.
Exceptions:

  • For infants, lower volume is acceptable (2-3 cc)
  • Patients with a hematological neoplasia, or who have had a bone marrow transplant , or peripheral stem cell transplant should not send blood (see “Saliva”)

DNA:  ~10 micrograms, high quality DNA
Saliva: saliva samples collected in Oragene saliva kit
Prenatal samples: 2 T25 flasks, cultured amniocytes or CVS (at least 75%).
             Keep at ambient temperature and ship by overnight courier to arrive in the  
             lab on M/Tu/W.

  • Note: Maternal cell contamination (MCC) studies are required and performed as part of the prenatal test; therefore, blood sample from Mom is also required ( if not previously tested in this lab).   

TESTS PERFORMED ON TUMORS

Microsatellite Instability (MSI)

Formalin-fixed, paraffin-embedded (FFPE) tissue samples.  Please submit:

  • 6 slides of tumor sample (10 micron serial sections, unstained; 1 H/E stained).
  • 6 slides of matched normal tissue (e.g. bordering tissue, normal lymph node(s), 10 micron unstained; 1 H/E stained).
  • Blood samples (if no normal tissue available): 2 tubes (6 ccs each) in ACD (yellow top) or EDTA (lavender top) tubes

Ensure that the slides are clearly labeled. A copy of the pathology report should accompany the specimen. Place slides in appropriate containers to ensure against breakage. Alternatively, the paraffin blocks may be submitted.

Immunohistochemistry (IHC)

Formalin-fixed, paraffin-embedded (FFPE) tumor tissue samples. Ensure that the blocks are clearly labeled. A copy of the pathology report should accompany the tissue block. We prefer to receive tissue blocks, but if they are not available, we will accept unstained slides (please submit one slide per antibody to be tested plus one for H&E, cuts must be 4 microns thick). Place slides in appropriate containers to ensure against breakage.

MLH1 somatic promoter methylation (MLH1-METH)

Formalin-fixed, paraffin-embedded (FFPE) tissue samples.   Please submit:

  • 6 slides of tumor sample (10 micron serial sections, unstained; 1 H/E stained).

Ensure that the slides are clearly labeled. A copy of the pathology report should accompany the specimen. Place slides in appropriate containers to ensure against breakage. Alternatively, the paraffin blocks may be submitted.

Tumor oncology (Onco48 panel, OncoFusions, Onco48-Plus-Fusions, EGFR, KRAS, BRAF, cKIT, PDGFRA, etc)

Formalin-fixed, paraffin-embedded (FFPE) tumor  tissue samples (providing  at least 3000 tumor cells and at least 30% tumor). Blocks are preferred (unused portion will be returned), but slides are also accepted. For slides, please submit:

  • 10 slides, 10 micron serial sections, unstained, without coverslip.
  • 1 representative H&E slide, 4 micron section, with a coverslip.

If the sample is a needle biopsy or clearly has very little tumor, please send additional slides. Ensure that the slides are clearly labeled. A copy of the pathology report should accompany the specimen. Place slides in appropriate containers to ensure against breakage.

Prosigna-Breast

Formalin-fixed, paraffin-embedded (FFPE) tissue from invasive breast carcinomas meeting clinicopathological criteria for testing. Blocks are preferred (unused portion will be returned), but slides are also accepted. For slides, please submit:

  • 10 slides, 10 micron serial sections, unstained, unbaked
  • 1 representative H&E slide, 4 micron section, with a coverslip (tumor area must be at least 4mm2 and with a minimum of 10% tumor cellularity)
Test Request Form

All tests require the CMDL Test Request Form (TRF) (download from the forms page ).

CPT Codes

Click here for a list of CPT codes in portable document format (pdf) .

Shipping Information

Please contact CMDL Client Service if you would like to request a specimen collection kit or shipping label. You must include a completed Test Request Form (TRF)  with every sample. If the Test Request Form (TRF)  is not complete, testing WILL be delayed. The CMDL is open Monday to Friday, 7:00 am to 5:00 pm Pacific time.

Please try to schedule specimen collection to ensure delivery during these hours. If it is necessary to schedule delivery during holidays and weekends, the samples will be held until working hours. Please note that, occasionally, for samples that are received on the weekends or holidays, a second sample is requested due to DNA extraction failure.

All specimens should be shipped to:

Clinical Molecular Diagnostic Laboratory
City of Hope
Northwest Building, Second Floor, Room 2236
1500 East Duarte Road
Duarte, CA 91010