Marcia Miller, Ph.D.
Location
Duarte Cancer Center
Duarte, CA 91010
Education & Experience
1972 - University of California Los Angeles, Ph.D., Botanical Sciences
1966 - University of California Davis, Davis, CA, M.A., Botany
1965 - University of California Davis, Davis, CA, B.S., Botany, with Highest Honors
1977-1978 - Biozentrum, University of Basel, Basel, Switzerland, Research Fellow, Cell Biology/Immunology
1972-1975 - Division of Biology, California Institute of Technology, Pasadena, CA, Research Fellow (Dr. Jean Paul Revel-Cell Biology/Immunology)
1969-1970 - Public Health Service Research Fellowship, UCLA
1968 - UCLA Medical Center Auxiliary Fellowship
1968-1972 - University of California, Los Angeles, CA, Pre-doctoral Fellow, Department of Botanical Sciences
1966 - National Science Foundation Predoctoral Fellowship, UC Davis
2002-Present - Associate Chair, Department of Molecular Biology, Beckman Research Institute of City of Hope, Duarte, CA
2000-Present - Professor, Center for RNA Biology and Therapeutics, Department of Molecular Biology, Beckman Research Institute of City of Hope, Duarte, CA
1994-2000 - Research Scientist, Department of Molecular Biology, Beckman Research Institute of City of Hope, Duarte, CA
1985-Present - Director, Electron Microscopy and Atomic Force Microscopy Core Facility, Beckman Research Institute of City of Hope, Duarte, CA
1986-1994 - Associate Research Scientist, Beckman Research Institute of City of Hope, Duarte, CA
1983 - Visiting Scientist, Basel Institute for Immunology, Basel, Switzerland
1979-1986 - Associate Research Scientist, City of Hope, Duarte, CA.
Research
Laboratory
Research in my lab is devoted to understanding genetic variability as it relates to heritable resistance to cancers and infections. We are especially interested in polymorphic genes that affect the occurrence of cancers associated with infection. Surprisingly, at least 20% of all human cancers are caused or progress as the result of infection. Viral infections, in particular, play a significant role in the occurrence of several types of tumors. It is likely that the diverse genetic makeup of modern human populations is, at least to some degree, a consequence of genetic selection by infection over evolutionary time. Hence, some individuals may be better equipped genetically than others when it comes to fighting infections by cancer causing microbes. Currently we are gathering evidence for this hypothesis by looking at cancer causing viral infections in our experimental animal model.
We are studying the genetics of resistance to the most oncogenic herpes virus known. This is the GaHV-2 herpes virus that causes Marek’s disease, a T-cell lymphoma in chickens. We have identified a single chicken gene BG1, a gene within the chicken MHC, as a major determinant of whether tumors form following GaHV-2 infection (Goto, Wang et al. PNAS 2009). Interestingly the two alleles in our study differ by a very small region of only 225 nucleotide base pairs (bp). The 225 bp are inserted into 3’-untranslated region of the allele that is associated with increased tumors. We are now investigating whether the role of BG1 in T cell maturation and whether the 225 bp insert is targeted by viral or cellular microRNA that suppresses expression of BG1 protein thereby increasing the likelihood GaHV-2-induced tumors.
In a second project also focused on polymorphic genes we are defining the function of avian MHC class I-related (MR1) molecules. We postulate that chicken molecules, like their human counterpart MR1, have a role in immune responses to bacteria. These avian genes may be a means for limiting the presence of foodborne bacterial pathogens in poultry products.
I also serve as director of the City of Hope Electron Microscopy and Atomic Core Facility where I assist members of the City of Hope scientific community in applying contemporary methods of electron microscopy in their work. We recently added serial block face scanning electron microscopy (SBF-SEM) as a new service. SBF-SEM is a powerful means for quantitative EM and for visualizing the three dimensional structure of cells and the structural interactions between cells in tissues. Other newly available methods include correlative light and electron microscopy (CLEM), scanning transmission electron microscopy (STEM) and elemental analysis by energy-dispersive X-ray spectroscopy (EDS). See the EM/AFM Core Facility website for more information.
Awards & Memberships
Awards
1975, Graduate Woman of the Year, UCLA
1965, Turtox-Croasdales Scholarship, MBL, Woods Hole, MA
1965, Herbert A. Young Medalist, Departmental Citation in Botany, PBK, UC Davis
Memberships
1992-Present, Editorial Board, Immunogenetics
Publications
- Zhang J, Goto RM, Miller MM. 2020. A simple means for chicken MHC-Y genotyping using short tandem repeat sequences. Immunogenetics 72:325-332. PMID:32488290
- Zhang Z, Le K, La Placa D, Armstrong B, Miller MM, Shively JE. 2020. CXCR2 specific endocytosis of immunomodulatory peptide LL-37 in human monocytes and formation of LL-37 positive large vesicles in differentiated monoosteophils. Bone Rep. 2019 Dec 13; 12:100237 PMID:31886324
- Song A, Dai W, Jang MJ, Medrano L, Li Z, Zhao H, Shao M, Tan J, Li A, Ning T, Miller MM, Armstrong B, Huss J M, Zhu Y, Liu Y, Gradinaru V, Wu X, Jiang L, Scherer PE, Wang QA. 2020. Low- and high-thermogenic brown adipocyte subpopulations coexist in murine adipose tissue. J Clin Invest. 130(1):247-257. PMID:31573981
- Lennon K, Wakefield D, Maddox A, Brehove M, Garcia-Mansfield K, Meechoovet, B, Reiman R, Hutchins E, Miller M, Goel A, Pirrotte P, Van Keuren-Jensen K, Jovanović-Talisman T. 2019. Single molecule characterization of individual extracellular vesicles from pancreatic cancer. Journal of Extracellular Vesicles, 8(1):1685634. PMID:31741725
- Iglesias GM, Canet ZE, Cantaro H, Miquel MC, Melo JE, Miller MM , Berres ME, Fulton JE. 2019. Mhc-B haplotypes in "Campero-Inta" chicken synthetic line. Poult Sci. 98(11):5281-5286. PMID:31376352
- Ahrens BJ, Li L, Ciminera AK, Chea J, Poku E, Bading JR4, Weist MR, Miller MM, Colcher DM, and Shively JE. 2017. Diagnostic PET Imaging of Mammary Microcalcifications using 64Cu-DOTA-alendronate in a Rat Model of Breast Cancer. The Journal of Nuclear Medicine. 58(9):1373-1379. PMID:28450564
- Warren, WC, Hillier LW, Tomlinson, C, Minx P, Kremitzki M, Graves T, Markovic C, Bouk N, Pruitt K, Thibaud-Nissen F, Schneider V, Mansour T, Brown CT, Zimin A, Hawken R, Pyrkosz AB, Morisson M, Fillon V, Vignal A, Chow W, Howe K, Fulton JE, Miller MM, Lovell P, Mello C, Wirthlin M, Mason AS, Kuo R, Burt DW, Dodgson JB, Cheng HH. 2017. A new chicken genome assembly provides insight into avian genome structure. G3 (Bethesda) 7:109-117. PMID:27852011
- Fulton JE, McCarron AE, Lund AR, Pinegar K, Wolc A, Chazara O, Bed’Hom B, Berres ME, Miller MM. 2016. A high-density SNP panel reveals extensive diversity, frequent recombination and multiple recombination hotspots within the chicken major histocompatibility complex B region between BG2 to CD1A1. Genetics Selection Evolution. 48:1. PMID:26743767
- Kjaerup RM, Dalgaard TS, Norup LR, Goto RM, Miller MM, Sorensen P, Juul-Madsen HR. 2014. Transcription efficiency of different chicken mannose-binding lectin promoter alleles. Immunogenetics 66(12):737-742. PMID:25343382
- Miller MM, Robinson CM, Abernathy J, Goto RM, Hamilton MK, Zhou H, Delany ME. 2014. Mapping genes to chicken microchromosome 16 and discovery of olfactory and scavenger receptor genes near the major histocompatibility complex. Journal of Heredity 105(2):203-215. PMID:24336927