The Aria SORP
(Special Order Research Product) is a six laser system (355nm, 405nm, 457nm, 488nm, 561nm & 640nm) with a maximum of 17 simultaneous fluorescent detectors. (See tech specs
) But the instrument is configured to have 19 fluorescent detectors to choose from. In addition to a standard forward scatter photodiode detector, the Aria SORP is equipped with a Photomultiplier tube (PMT) for forward scatter. This additional parameter allows for detection of particles as small as 0.4 microns. Samples can be loaded onto the sorter in a variety of tube sizes (microfuge tubes, 12X75mm or 15ml conical centrifuge tubes). The possible nozzle tip sizes available for sorting are 70 micron, 85 micron, 100 micron, and 130 micron. Bulk sorting of 1 to 4 populations per sample is possible. Collection tubes for 1 or 2 way sorting are 15ml, 12X75mm, or 1.5ml microfuge tubes. With 3 or 4 way sorting, either 12X75mm or 1.5ml microfuge collection tubes are utilized. Also, an Automatic Cell Deposition Unit (ACDU) is available for sorting onto slides and plates (6, 24, 48, 96 or 384-well plates). With the ACDU single cell or any user specified number of cells can be collected. The Aria SORP is equipped with temperature control for both the sort sample and the sort collection tubes. This sorter is contained in a Biosafety Cabinet.
Cytometer Setup and Tracking (CS&T) beads are purchased from BD Bioscience and run on a daily basis to monitor system performance. Also Accudrop beads (BD Bioscience) are utilized to setup the sorting parameters of the system prior to cell sorting for a given day.
Investigators must supply their own reagents. All samples are filtered by core staff prior to placement on the cell sorter. We utilize 60micron nylon mesh that has been autoclaved for sterility. It is recommended that the user provides collection tubes with the appropriate media for their specific cell type. For plate sorting, the user must provide the appropriate plate type and collection media. It is also recommended that the user bring extra collection media.