S3 Cell Sorter (Bio-Rad): 2 colinear lasers (488nm and 561nm) with 4 fluorescent detectors. This sorter has the capacity to sort 2 simultaneous populations into tubes. Temperature control of sample and sorted material available and the system is contained in a Class 1 Bio-Bubble.
ProLine beads are purchased from Bio-Rad and run on a daily basis to monitor system performance. These beads are also utilized to setup the sorting parameters of the system prior to cell sorting for a given day.
Investigators must supply their own reagents. All samples are filtered by core staff prior to placement on the cell sorter. We utilize 60micron nylon mesh that has been autoclaved for sterility. It is recommended that the user provides collection tubes with the appropriate media for their specific cell type. For plate sorting, the user must provide the appropriate plate type and collection media. It is also recommended that the user bring extra collection media.