By setting up the HTS Core in-house, we can provide timely assistance to City of Hope investigators regarding HTS assays development, validation and implementation. This allows for centralized quality control of drug screening and development. In addition, the HTS Core works closely with the Synthetic and Biopolymer Chemistry Core and the Biomedical Informatics Core in identifying, improving and developing lead compounds, which in turn benefit chemical biology and drug discovery research at City of Hope.
The HTS Core has deployed a series of informatics programs and implemented standard operating procedures to ensure proper management of the large amount of information it handles regularly. These steps also foster efficient information exchange between the core and its users.
Laboratory Information Management System
The HTS Core has set up a centralized database, built upon the Laboratory Information Management System developed by Cheminnovation called Chemical and Biological Information System (CBIS).
CBIS provides a Web-based application for managing chemical and biological materials and data throughout different cores and laboratory scientists, enabling insights resulting from high-volume data mining.
CBIS is hosted on the Biomedical Informatics Core’s high-availability Dell PowerEdge 2950, two dual-core 64-bit Xeon servers with 4GB RAM each and 1-terabyte SAN network storage space. CBIS’s integrated open framework of Web solutions has a user-friendly front end that can incorporate many external data mining applications and transparently link to relational database back end solutions.
Compound Registration
All compounds, regardless of source, are entered into the database and assigned a unique ID number, which facilitates tracking of the compound as it passes through various assays and tests.
Reproducibility and robustness of each HTS assay is documented, e.g. by Z-factor analysis.
Statistical Analysis
S-PLUSsoftware, from Insightful Inc., is an industry-standard solution for exploratory data modeling and statistical analysis. With over 4,000 data analysis functions, including the most comprehensive and robust set of modern methods, S-PLUS is used on all data to ensure its quality and significance.
Information Exchange/Sharing
All high quality data is deposited into the central database in a timely manner to facilitate information exchange/sharing and discussions among researchers.