The Synthetic and Biopolymer Chemistry Core (SBCC) equipment is comprehensive in both small and large molecule synthesis capabilities and state-of-the-art spectroscopic instrumentation for structural characterization. In addition, the lab is equipped with in-house custom-built instruments adapted for specialized applications in the area of biopolymer synthesis.
Biopolymer Synthesis
The Synthetic and Biopolymer Chemistry Core maintains several nucleic acid and peptide synthesizers that are adapted for both routine andhighly specialized custom syntheses. These include:
Two Automated DNA/RNA Synthesizers. These machines are fully adaptable for custom synthesis to include modified bases as well as other non-natural base and sugar analogs.
Two Semi-Automated Peptide Synthesizers. These are state-of-the-art, in-house designed and built, semi-automated peptide synthesizers. These custom instruments provide much greater mechanical flexibility and efficiency in the coupling processes that allow for the introduction of difficult-to-incorporate modified amino acid derivatives. Long polypeptides containing >100 amino acids have been synthesized on these instruments, which were unachievable by commercial vendors.
Waters HPLC Instrumentation. The HPLC system is specifically adapted for purification of small to relatively large amounts of DNA/RNA and peptides.
Small Molecule Synthesis
Virtually all of the small molecule projects involve custom synthesis to varying degrees. A full array of state-of-the-art small molecule instrumentation is available for complete structural characterization. These include:
Agilent Analytical and Preparatory HPLCs. In addition to the standard features, these instruments possess chiral separation capabilities that enable the purification and resolution of racemic material providing single enantiomers with high purity.
Varian High-Field 400 MHz NMR. This instrument is capable of one- and two-dimensional data analysis with cryoprobe capability for added sensitivity. Higher field NMR instrumentation (500 and 600 MHz) also exists on campus when the need for greater magnet strength arises.
JEOL AccuTOF™-DART™ Mass Spectrometer. This new MS design is capable of exact mass measurements under atmospheric conditions with no sample preparation and instantaneous detection. The precision of this new instrument is unmatched for the determination of small-molecule molecular mass of virtually all compound classes.
FTIR, UV/VIS and Polarimeter. Spectroscopic instrumentation used for the structural identification of organic functional groups, chromophores, and optical rotation measurements, respectively.