3DEM: Serial block face SEM

A tumor imaged by serial block face SEM (inset: 3D serial block face dataset of the same tumor)
3D reconstruction, segmentation, surface rendering

Mammary duct ultrastructure in 3D. View the full movie.


Elemental anylysis: Energy-dispersive x-ray spectroscopy

Gold nanoparticles coated with PEG
Ultra-thin Section from Resin Embedded Samples for TEM

Ultra-thin Section from Resin Embedded Samples for TEM

The most commonly used approach to obtaining ultrastructural details of cells and tissues.
Cryo-EM of Plunge-Frozen Samples

Frozen-hydrated liposomes.
Toluidine Blue Stained 1-um-thick Section

One-micron sections provide an overview as illustrated here with a colony of pancreatic stem cells.
Negative Staining

Negative stained exosomes.


High Pressure Freezing and Freeze Substitution

Compared to harsh chemical fixation, HPF-FS provides superior preservation of cellular structure.


Immuno-gold labeling on specimen prepared by high pressure freezing/freeze substitution

Indirect immunolabeling with antibody conjugated 10-nm gold particles was used to monitor uptake of FITC-CpG-siRNA.
Immunogold Labeling SEM

In the SEM the immunogold particles are bright. Here immunogold labeling reveals CEACAM-1 associated with microvilli on the cell surface.


Visualization of Nucleic Acid

Rotary shadowing of spread DNA


Scanning Electron Microscopy

Surface structure of a monocyte


Atomic Force Microscopy

Structure of mRNA molecules (~1000 nt)


Transmission Electron Microscopy

Ultra-thin section of avian LMH cells prepared with membrane-enhancing reagents.


Transmission Electron Microscopy

Ultra-thin section of an avian LMH cell. Sample preparation was done on an adhesive slide using only ~2000 cells. This method can be used to image small numbers of cells (for example, directly from flow cytometry).


Transmission Electron Microscopy

Single wall carbon nanotubes with a diameter of about 1.4 nm resolved in the Tecnai 12 transmission electron microscope.