The Light Microscopy Core facility offers professional software for image quantification and 3D segmentation and reconstruction. We offer training and support for our software platforms including Image Pro Premier (Media Cybernetics, Rockville, MD). We have a server-based licensing strategy that allows scientists to use Image Pro Premier from their own workstations. We also offer high-end imaging computers in the main core facility for more demanding data sets.
For 3D deconvolution we use Autoquant X3 (Media Cybernetics, Rockville, MD). This software is available on our high-end imaging workstations and can deconvolve imaging data from widefield, Confocal or 2-Photon microscope systems.
For 3D segmentation and reconstruction we use Amira with “Microscopy Pack” (FEI, Hillsboro, OR). This software has some of the best 3D segmentation tools and can handle large data sets from widefield, Confocal or 2-Photon Z-stacks, as well as DICOM and other formats. The powerful Digital Director modality can be used to create complex 3D movies.
We also offer support for other imaging software programs for example Zen Blue, Zen Black and Prairie View.