Prairie Ultima 2 Photon Microscope

• Transmitted and Fluorescence Illumination (Exfo Xcite)
• Upright Configuration
• Custom Heated Stage Insert

Laser Scanning Mode

• Galvo; for high quality imaging
• AOD; for fast imaging, e.g., 25 fps
• 4 Above Stage PMT Detectors (primary)
• 2 Substage Detectors (secondary)

Imaging Features

• Motorized stage: X, Y, and Z for optical sectioning
• 16 bit TIFF images
• 128, 256, 512, 1024, 2048 pixel resolution
• Frame Averaging
• Adjustable Dwell Time
• Advanced Time Series and Z-Sectioning
• Variable Zoom
• Atlas Mosaic Imaging

Coherent Laser

• Coherent Chameleon Ultra II
• Fast Tuning from 680nm - 1080nm
• Power output approximately 3000mW at 800nm
• Capable of exciting all fluorophores in the visible spectrum

Objectives:

• 10x/0.3 Dry WD 10mm,
• 20x/1.0 W Imm (XLUMPLN) WD 2mm,
• 40x/IR-2/0.8 W Imm WD 3.3mm,
• 60x/IR-2/0.8 W Imm WD 3.3mm

Spectra

• View PMT Detectors and Spectra