Recent Projects

The following recent publications illustrate the technical capabilities of the laboratory and a variety of collaborative projects.
Murai K, Sun G, Ye P, Tian E, Yang S, Cui Q, Sun Gu, Trinh D, Sun O, Hong T, Wen Z, Kalkum M, Riggs AD, Song H, Ming, G-L, and Shi Y. 2016. The TLX-miR-219 cascade regulates neural stem cell proliferation in neurodevelopment and schizophrenia iPSC model. Nature Communications 7:10965. doi: 10.1038/ncomms10965.
Champer, J., Ito, J.I., Clemons, K.V., Stevens, D.A., Kalkum M., Proteomic Analysis of Pathogenic Fungi Reveals Highly Expressed Conserved Cell Wall Proteins (2016). Journal of Fungi 2: 6. doi:10.3390/jof2010006

Mahadev, V., Starr, R., Wright, S. L., Martinez, C., Jensen, M. C., Barish, M. E., Forman, S.J., Brown, C.E., (2014). Cytokine Induction of VCAM-1 but Not IL13Rα2 on Glioma Cells: A Tale of Two Antibodies. PLoS ONE, 9(5), e95123.

Chow, A., Zhou, W., Liu, L., Fong, M. Y., Champer, J., Van Haute, D., et al. (2014). Macrophage immunomodulation by breast cancer-derived exosomes requires Toll-like receptor 2-mediated activation of NF-κB. Scientific Reports, 4, 5750.

Figarola, J. L., Singhal, P., Rahbar, S., Gugiu, B. G., Awasthi, S., & Singhal, S. S. (2013) COH-SR4 reduces body weight, improves glycemic control and prevents hepatic steatosis in high fat diet-induced obese mice. PLoS ONE, 8(12), e83801.

Lee, T. D., Moore, R. E., & Keen, D. (2013). Introducing samples directly into electrospray ionization mass spectrometers by direct infusion using a nanoelectrospray interface. Current Protocols in Protein Science / Editorial Board, John E Coligan  [Et Al], 74, 16.8.1–7.

Sharad S Singhal, James Figarola, Jyotsana Singhal, Kathryn Leake, Lokesh Nagaprashantha, Christopher Lincoln, B Gabriel Gugiu, David Horne, Richard Jove, Sanjay Awasthi, Samuel Rahbar, (2012) 1,3-Bis(3,5-dichlorophenyl) urea compound 'COH-SR4' inhibits proliferation and activates apoptosis in melanoma. Biochemical Pharmacology 84 (11) pp. 1419-27.

Champer, J., Diaz-Arevalo, D., Champer, M., Hong, T. B., Wong, M., Shannahoff, M., et al. (2012). Protein targets for broad‐spectrum mycosis vaccines: quantitative proteomic analysis of Aspergillus and Coccidioides and comparisons with other fungal pathogens. Annals of the New York Academy of Sciences, 1273(1), 44–51.

Ashley M Crane, Hong-Uyen Hua, Andrew D Coggin, Bogdan G Gugiu, Byron L Lam, Sanjoy K Bhattacharya, (2012) Mass spectrometric analyses of phosphatidylcholines in alkali exposed corneal tissue. Invest Ophthalmol Vis Sci 53, pp. 7122-7130.
Jiehui Deng, Yong Liu, Heehyoung Lee, Andreas Herrmann, Wang Zhang, Chunyan Zhang, Shudan Shen, Saul J Priceman, Maciej Kujawski, Sumanta K Pal, Andrew Raubitschek, Dave S B Hoon, Stephen Forman, Robert A Figlin, Jie Liu, Richard Jove, Hua Yu, (2012) S1PR1-STAT3 signaling is crucial for myeloid cell colonization at future metastatic sites. Cancer Cell 21 (5) pp. 642-54.
Khue Truong, Terry D. Lee and Yuan Chen, Small Ubiquitin-like Modifier (SUMO) Modification of E1 Cys Domain Inhibits E1 Cys Domain Enzymatic Activity (2012). Journal of Biological Chemistry 287 (19) pp. 15154-15163.
Carissa M Thomas, Teresa Hong, Jan Peter van Pijkeren, Peera Hemarajata, Dan V Trinh, Weidong Hu, Robert A Britton, Markus Kalkum, James Versalovic, (2012) Histamine derived from probiotic Lactobacillus reuteri suppresses TNF via modulation of PKA and ERK signaling. PLoS ONE 7 (2) pp. e31951.
Lei Zhang, George S Katselis, Roger E Moore, Kossi Lekpor, Ronald M Goto, Terry D Lee, Marcia M Miller, (2011) Proteomic Analysis of Surface and Endosomal Membrane Proteins from the Avian LMH Epithelial Cell Line. J Proteome Res 10 (9) pp. 3973-82.
Zhanhong Wu, Ivan Todorov, Lin Li, James R Bading, Zibo Li, Indu Nair, Kohei Ishiyama, David Colcher, Peter E Conti, Scott E Fraser, John E Shively, Fouad Kandeel, (2011) In vivo imaging of transplanted islets with 64Cu-DO3A-VS-Cys40-Exendin-4 by targeting GLP-1 receptor. Bioconjug Chem 22 (8) pp. 1587-94.
Diana Diaz-Arevalo, Karine Bagramyan, Teresa B Hong, James I Ito, Markus Kalkum, (2011) CD4+ T cells mediate the protective effect of the recombinant Asp f3-based anti-aspergillosis vaccine. Infection and Immunity 79 (6) pp. 2257-66.
Kenneth J Dery, Shikha Gaur, Marieta Gencheva, Yun Yen, John E Shively, Rajesh K Gaur, (2011) Mechanistic control of carcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM1) splice isoforms by the heterogeneous nuclear ribonuclear proteins hnRNP L, hnRNP A1, and hnRNP M. J Biol Chem 286 (18) pp. 16039-51.
Lin Li, Desiree Crow, Fabio Turatti, James R Bading, Anne-Line Anderson, Erasmus Poku, Paul J Yazaki, Jenny Carmichael, David Leong, Michael P Wheatcroft, Andrew A Raubitschek, Peter J Hudson, David Colcher, John E Shively, (2011) Site-specific conjugation of monodispersed DOTA-PEGn to a thiolated diabody reveals the effect of increasing peg size on kidney clearance and tumor uptake with improved 64-copper PET imaging. Bioconjug Chem 22 (4) pp. 709-16.
Daniel Tamae, Punnajit Lim, Gerald E Wuenschell, John Termini, (2011) Mutagenesis and Repair Induced by the DNA Advanced Glycation End Product N(2)-1-(Carboxyethyl)-2'-deoxyguanosine in Human Cells. Biochemistry 50 (12) pp. 2321-2329.
Li Zheng, Jia Jia, L David Finger, Zhigang Guo, Cindy Zer, Binghui Shen, (2011) Functional regulation of FEN1 nuclease and its link to cancer. Nucleic Acids Res 39 (3) pp. 781-94.
Serina Ortiz, Wenhui Lee, David Smith, Stephen J Forman, Terry D Lee, Chih-Pin Liu, (2010) Comparative analyses of differentially induced T-cell receptor-mediated phosphorylation pathways in T lymphoma cells. Exp Biol Med (Maywood) vol. 235 (12) pp. 1450-63.