Using The Facility


The Mass Spectrometry and Proteomics Core is available for  “shared use” (for trained internal users only) and on “full service” basis. Investigators should contact the Mass Spectrometry and Proteomics Core Facility manager, Gabriel Gugiu at 800-826-4673, x63905 to discuss the experimental strategy for any sample analysis prior to sample submission.
 
Shared Use:
Instrumentation of the facility is available to City of Hope and Beckman Research Institute users on a shared use basis. Users have to be trained by core facility personnel on the instrument appropriate for their particular project before core resources can be accessed.

How to Prepare Samples:
Please see the guidelines for preparing samples in order to obtain a successful analysis. Direct any concerns to the Mass Spectrometry and Proteomics Core Facility manager, Gabriel Gugiu at 626-256-HOPE (4673) x63905 prior to sample submission.
 
Supplies Needed:
With few exceptions (quantitation or method development standards and analytical HPLC columns), it is not necessary for the investigator to provide supplies for most common analyses and projects carried out by the Mass Spectrometry and Proteomics Core. The cost of common reagents and solvents is built into the fee schedule.
 
Turn-around Time:
The turn-around time for the mass analysis of a single sample is five to seven days for nanoelectrospray MS data for samples of low complexity and 7-10 days for more complex analyses. For samples submitted for LC/MS/MS analysis with database search and/or quantitation, typical turn-around time is about two weeks (backlog dependent). For samples that require method development, investigators will be apprised of the anticipated time required to obtain results of the requested analyses on a per sample basis when experimental details are discussed with the facility manager.
 
Training
 
All users who wish to analyze their own samples must be trained by a member of the MS facility prior to their intended use of the instrument. Please contact us to schedule a training session.
 
Rules for instrument use:
Users must be trained in the specific technique and instrument they wish to use. Any other use must be discussed with the manager. Any fault observed with the instruments must promptly be communicated to the facility personnel to address the problem.
 
Signup for instrument time
 
Instrument sign-up is handled through iLab. The signup rules below must be followed at all times.
 

  • Instrument maintenance and repair takes precedence over other uses. Please discuss any concerns with us. We will attempt to accommodate you as best as we can.
  • The Orbitrap Fusion nanoelectrospray mode is only intended for accurate mass determination for small molecules, peptide and oligonucleotide mass and sequence determinations and is available one day a week. The rest of the time is dedicated to LCMS analyses. All other nanospray analyses that don’t require high mass accuracy can be performed on the LTQ instrument any day of the week.
  • Mondays are reserved for Orbitrap Fusion, Synapt G2 HDMS and Agilent QQQ maintenance and calibration. Tuesdays and Thursdays are reserved for Agilent Q-TOF1 and 2 maintenance and calibration.
  • LC/MS/MS time may be signed up for in 1-3-day blocks. If you need less time than that, sign up only for the number of days you need so that others can use the instrument. Exceptionally, more time can be reserved if the project requires it and if available, upon approval of the MS facility personnel.
  • For quantitation projects on any instrument it is allowed to reserve one-week block time (the time between two consecutive maintenance days).
  • No one may sign up for more than one week in any calendar month on a single instrument. For scheduling purposes, the first day of the time block determines its month.
  • No one group/laboratory may sign up for more than 6 consecutive days on one instrument in one calendar month without core personnel approval.
  • If any time blocks are not signed up a week in advance they can be used by anybody even if they are in violation of the above rules.