The scope of the work performed by the core facility ranges from cell culture, through animal studies, through clinical trials; (i.e., Basic Science through Application). The core facility focuses primarily on proteomic and glycomic approaches to biomarker discovery, but has capabilities to investigate the contribution of lipid and metabolic biomarkers. Biomarkers relying on nucleic acid expression or genome sequencing technologies are not performed by our core facility, but are performed by a separate Genomics Core facility. The core also can readily assist in the development of preclinical assays for validating the efficacy of the biomarkers discovered.
Bruker Ultraflex Mass Spectrometer
Mass profiling–Molecular Signatures
Negative and positive ion modes
Determine mass of molecules from < 500->200,000+ daltons
Perform mass profiling & molecular localization on FFPE or frozen tissue samples.
Reproducible spotting of samples or liquids of interest in Biomarker Discovery Projects.
Perform ELISA assays
Column fractionation of clinical or mouse model samples using 96 well format filter plates can be performed to enrich components of a specific mass thought to be biomarkers of interest.
Tecan Freedom EVO
The robotic platform offers an advanced, proven and reliable liquid handling system for different scales and throughputs. With the choice of various liquid handling and manipulative robotic arms we provide an extendable work area and variable configurations. Freedom EVO workstations can automate a diverse range of applications including primary and secondary screening, cell growth and maintenance, nucleic acid sample preparation, hit picking, library management and sample weighing.
Nanoflow HPLC purification of complex mixtures of intact proteins from cell lysates and protein expression studies, proteolytic fragments and clinical samples. Useful for small volume (1-10 ul) protein fractionation.
Protein Purification over traditional chromatography columns.Capable of purifying large quantities of protein or small quantities in a project specific format.
Reproducibly purify unlimited quantities of proteins, protein complexes, cells, miRNA’s, organelles, and other biological materials under denaturing or non-denaturing conditions using a 96 well format.
Performs IEF or Zonal Electrophoresis.
Separations can be optimized to occur within minutes; aiding labile PTM recovery.
Development of manual tissue staining protocols for determining biomarker specificity and sensitivity.
Automated tissue staining
Assay Development for Selective Tissue staining
IHC and ISH capable