Molecular Pathology

Molecular Pathology facilitates, implements, and supports the needs of basic science researchers, clinical researchers, population researchers, and clinical science collaborations. 
 
Interactions between basic scientists and clinicians are fostered to ensure the inclusion of scientifically sound, correlative studies in clinical trials—and to enable and assist research studies using clinical samples from patients who are enrolled in clinical trials. 
 
Across all of its efforts, Molecular Patholgoy places its emphasis on the pursuit of institutional collaborations, seeking and enabling opportunities for TRL and other Core laboratories to work closely together.
 
Services offered:
 
  • Bio-specimen repository of samples for clinical and population research protocol
  • Receipt, processing, and storage of human samples
  • Database management system to centralize information
 
  • Creation of cell lines from tumor
 
  • Studies of circulation of tumor cell (CTC) and other rare events
  • CTC enumeration and enrichment
  • Single CTC isolation, whole-genome amplification, from a single cell, for genetic characterization
  • Pure population collection from Formalin-fixed, paraffin-embedded (FFPE) tissue samples
  • DNA preparation for genetic characterization
  • Sorting of rare living cells for RNAseq analysis
 
  • Molecular biology-based translational studies
  • Cell proliferation assay and drug cytotoxicity analyses
  • Isolation and quantification of DNA and RNA
  • Design and optimization of real-time polymerase chain reaction (PCR) analysis
  • Protein isolation, western blot, and multiplex enzyme-linked immunosorbent assay (ELISA)
  • Other molecular biology-based assays