A National Cancer Institute-designated Comprehensive Cancer Center

Make an appointment: 800-826-HOPE
Pathology Bookmark and Share

Pathology

Led by pathologists renowned for diagnostic excellence, the Department of Pathology at City of Hope combines state-of-the-art laboratories equipped with the latest diagnostic techniques and advanced instrumentation with superior investigative skills to accurately and rapidly identify even the rarest and most complex diseases. Our experts provide comprehensive services, from initial diagnosis to monitoring of prognostic indicators throughout the treatment process. Our team also maintains a blood bank and offers a full complement of services relating to blood and bone marrow donors.
 
Pathology Consultation Services
Our Pathology Consultation Services offers a comprehensive program to fulfill all pathology needs. Physicians at City of Hope and from around the world choose to submit their biopsy samples here because our laboratories are of the highest caliber and our team of pathologists are recognized experts in diagnosing and monitoring disease.
 
Our team takes a coordinated and cooperative approach, and final interpretations are only made at a daily consensus conference of senior staff, reinforcing the validity of the initial pathologist’s opinion and minimizing any possibility of error.
 
Rapid, Integrated Results at Competitive Prices
Our highly efficient support staff – from receptionists to transcriptionists to managers – helps deliver complete, accurate reports quickly to the requesting physician. The results of multiple tests are combined into one integrated report, for quick, clear access to priority tests. In most cases, results are obtained within 24 to 48 hours, with automatic faxing capabilities once the reports are signed out. As a nonprofit institution, we are able to offer highly competitive prices for our services. Our regular rates are low, and contracts can be negotiated for prospective high-volume clients. Professional revenues generated are used to support the department’s research activities.

 

 

Pathology Case Submission

Cases submitted to a specific pathologist will be read by the designated party. Otherwise we will assign the case to an appropriate investigator.

Always include the patient's clinical history along with referring physician's name, phone and fax numbers on the requisition form.

Send cases to:

City of Hope
Attn: Anatomic Pathology
1500 E. Duarte Road
Duarte, CA 91010

 

 

Pathology Customer Service

Customer and Financial Services
We are committed to providing superior pathology consultation services and supporting your needs in an increasingly competitive and cost-conscious medical marketplace.
 
To that end, we endeavor to complete your case as quickly as technically possible while maintaining our high quality standards.
 
For your convenience, we will bill third party payers. Simply complete the insurance information on the requisition form. In the absence of complete third party information, the referring pathologist or facility will be billed.
 
Unfortunately, due to Medicare regulations, we are not able to bill a third party payer if the patient is an inpatient at the referring facility. In this case, the referring institution will be billed. However, we do offer contract prices for these cases with highly competitive rates.
 
Specimen Collection
We offer free courier services for facilities located in the Southern California area. For facilities outside of Southern California, please call 626-256-HOPE (4673), ext. 63875, to coordinate pick up.

 

 

Pathology Laboratories

Immunochistochemistry
Our immunohistochemistry laboratory includes three high-volume automated immunostainers using highly advanced epitope retrieval techniques.  We offer complete immunohistochemistry characterization of surgical and cytologic specimens, including estrogen and progesterone receptors and over 100 other monoclonal and polyclonal antibodies. Each year, our hematopathology laboratory performs cytochemistry on more than 1,000 cases of acute leukemia and processes thousands of bone marrow specimens annually.
 
Flow Cytometry
Our flow cytometry laboratory uses high-capacity instruments to analyze thousands of samples annually to measure a variety of parameters, including cell surface antigens. The presence or absence of certain cell surface antigens has shown great prognostic value in various neoplasia, where treatment protocols can be optimized by customizing the type of treatment based on the types of cell surface antigens present. We provide comprehensive cytochemical and immunophenotypic characterization of leukemias and lymphomas; B and T cell subset analysis for immunodeficiencies; stem cell counts; solid tumor DNA content and cell cycle analysis.
 
Cytogenetics
Every year, the cytogenetics laboratory processes a heavy volume of neoplastic specimens, utilizing both conventional cytogenetics as well as fluorescence in situ hybridization (FISH). FISH has become the recognized gold standard in determining the likely course of disease and appropriate treatment in leukemias such as chronic lymphocytic.
 
Molecular Biology
Our molecular biology laboratory performs Southern blot hybridization, polymerase chain reaction, and in situ hybridization studies for B and T cell gene rearrangements, many different chromosomal translocations for lymphoma and leukemia, including t(14;18), t(9;22), t(15;17), and Epstein-Barr virus. Many of the molecular tests can be performed on paraffin-embedded tissue.
 
Laboratory accreditation, certification and licensing
The Consultation Services Laboratories at City of Hope are fully accredited by the College of American Pathologists (CAP #22900-01), certified under the Clinical Laboratory Improvements Amendments of 1988 (CLIA '88, ID No. 05D0665695) and licensed by the state of California (CLF 268).

The laboratories participate in the CAP's external proficiency survey program for flow cytometry, immunophenotyping, immunohistochemistry, cytogenetics and molecular oncology. Specific details on our survey performance are available upon request.
 

Antibody Library

Actin
AFP (alpha-fetprotein)
ALK protein
bcl-1
bcl-2
bcl-6
BerEp4
BG8
Calcitonin
Calretinin
CD1
CD2
CD3
CD4
CD5
CD7
CD8
CD10
CD15
CD16
CD20
CD21
CD23
CD30
CD31
CD34
CD35
CD343
CD45RA
CD45/45RB
CD45RO
CD56
CD57
CD68
CD79A
CD99
CD117 (c-kit)
CD138
CEA-Gold1
CEA-Gold5
c-erb-2
Chromogranin
Collagen-IV
DBA.44
Desmin
EBV-LMP
EMA (epithelial membrane  antigen)
ER (estrogen receptor)
Factor XIII (vWF)
FASCIN
GCDFP-15
GFAP
Granzyme B
HBME-1
HCG
Helico
Hemoglobin A
HLA-DR
HMB45
Inhibin
Kappa
Keratin 5/6
Keratin 8/18
Keratin 7
Keratin 8
Keratin 14
Keratin 19 (AE-1)
Keratin 20
Keratin cocktail (pan keratin)
34 βE 12 (HMW Keratin)
Ki-67
Lambda
Lysozyme
Melan-A
mes-13
MOC
MPX (myeloperoxidase)
NF (neurofilament)
p53
PacP (prostate acid phosphatase)
Perforin
PLAP (placential alkaline phosphatase)
PR (progesterone receptor)
Prolactin
PSA (prostate specifice antigen)
S100
SMA (smooth muscle actin)
Surfactant
Synaptophysin
Tdt
TGB (thyroglobin)
Thrombomodulin
TIA-1
TRAP
Tryptase
TTF-1
Villin
Vimentin
vWF (factor VIII)

Specimen Draw and Shipping Requirements

Cytogenetics
Results for STATs, pre-BMT's and newly diagnosed leukemias are available within seven days. All others are available in seven to 10 days but our goal is to report results as soon as they are available. Complex studies may require additional days. Specimens are accepted Monday through Friday, 8 a.m. to 5:30 p.m.
 
Drawing specifications
  • Leukemia and myelodysplastic syndromes: A bone marrow aspirate is the specimen of choice in most cases of leukemia, myelodysplastic and myeloproliferative disorders.
  • Leukemia cytogenetics: Obtain 1 to 2 ml of bone marrow (minimum 0.5 ml) in a heparinized syringe (approximately 500 USP sodium heparin per ml of specimen) and transfer the specimen into a green top sodium heparin tube. Keep at room temperature and ship as soon as possible.
  • Leukemic peripheral blood: Draw 5 to 20 ml of peripheral blood into a green top sodium heparin tube. Mix well by inverting. Keep at room temperature and ship as soon as possible.
  • Lymphoma and solid tumor cytogenetics: Bone marrow core biopsies should be placed in HBSS or RPMI medium.
 
Flow cytometry / molecular diagnostics
The laboratory is open Monday through Friday, 8 a.m. to 5 p.m.
 
Drawing specifications
  • Peripheral blood: For leukemia and lymphoma panels, draw six green top tubes (sodium herapin) and one purple top tube. Maintain at room temperature and ship as soon as possible. ACD tubes are acceptable. Sodium lithium should not be used.
  • Bone marrow: Draw at least one purple top tube, maintain at room temperature and ship as soon as possible. ACD and sodium heparin tubes are acceptable.
 
 

Pathology Team

Support this program

It takes the help of a lot of caring people to make hope a reality for our patients.  City of Hope was founded by individuals’ philanthropic efforts 100 years ago. Their efforts — and those of our supporters today — have built the foundation for the care we provide and the research we conduct. It enables City of Hope to strive for new breakthroughs and better therapies, ultimately helping more people enjoy longer, better lives.

For more information on supporting this specific program, please contact our Philanthropy Department at 800-667-5310 or philanthropy@coh.org. Or, to make a gift that supports all the research at City of Hope, donate online now.
 
We thank you for your support.
 
 
 

Pathology

Pathology

Led by pathologists renowned for diagnostic excellence, the Department of Pathology at City of Hope combines state-of-the-art laboratories equipped with the latest diagnostic techniques and advanced instrumentation with superior investigative skills to accurately and rapidly identify even the rarest and most complex diseases. Our experts provide comprehensive services, from initial diagnosis to monitoring of prognostic indicators throughout the treatment process. Our team also maintains a blood bank and offers a full complement of services relating to blood and bone marrow donors.
 
Pathology Consultation Services
Our Pathology Consultation Services offers a comprehensive program to fulfill all pathology needs. Physicians at City of Hope and from around the world choose to submit their biopsy samples here because our laboratories are of the highest caliber and our team of pathologists are recognized experts in diagnosing and monitoring disease.
 
Our team takes a coordinated and cooperative approach, and final interpretations are only made at a daily consensus conference of senior staff, reinforcing the validity of the initial pathologist’s opinion and minimizing any possibility of error.
 
Rapid, Integrated Results at Competitive Prices
Our highly efficient support staff – from receptionists to transcriptionists to managers – helps deliver complete, accurate reports quickly to the requesting physician. The results of multiple tests are combined into one integrated report, for quick, clear access to priority tests. In most cases, results are obtained within 24 to 48 hours, with automatic faxing capabilities once the reports are signed out. As a nonprofit institution, we are able to offer highly competitive prices for our services. Our regular rates are low, and contracts can be negotiated for prospective high-volume clients. Professional revenues generated are used to support the department’s research activities.

 

 

Pathology Case Submission

Pathology Case Submission

Cases submitted to a specific pathologist will be read by the designated party. Otherwise we will assign the case to an appropriate investigator.

Always include the patient's clinical history along with referring physician's name, phone and fax numbers on the requisition form.

Send cases to:

City of Hope
Attn: Anatomic Pathology
1500 E. Duarte Road
Duarte, CA 91010

 

 

Pathology Customer Service

Pathology Customer Service

Customer and Financial Services
We are committed to providing superior pathology consultation services and supporting your needs in an increasingly competitive and cost-conscious medical marketplace.
 
To that end, we endeavor to complete your case as quickly as technically possible while maintaining our high quality standards.
 
For your convenience, we will bill third party payers. Simply complete the insurance information on the requisition form. In the absence of complete third party information, the referring pathologist or facility will be billed.
 
Unfortunately, due to Medicare regulations, we are not able to bill a third party payer if the patient is an inpatient at the referring facility. In this case, the referring institution will be billed. However, we do offer contract prices for these cases with highly competitive rates.
 
Specimen Collection
We offer free courier services for facilities located in the Southern California area. For facilities outside of Southern California, please call 626-256-HOPE (4673), ext. 63875, to coordinate pick up.

 

 

Pathology Laboratories

Pathology Laboratories

Immunochistochemistry
Our immunohistochemistry laboratory includes three high-volume automated immunostainers using highly advanced epitope retrieval techniques.  We offer complete immunohistochemistry characterization of surgical and cytologic specimens, including estrogen and progesterone receptors and over 100 other monoclonal and polyclonal antibodies. Each year, our hematopathology laboratory performs cytochemistry on more than 1,000 cases of acute leukemia and processes thousands of bone marrow specimens annually.
 
Flow Cytometry
Our flow cytometry laboratory uses high-capacity instruments to analyze thousands of samples annually to measure a variety of parameters, including cell surface antigens. The presence or absence of certain cell surface antigens has shown great prognostic value in various neoplasia, where treatment protocols can be optimized by customizing the type of treatment based on the types of cell surface antigens present. We provide comprehensive cytochemical and immunophenotypic characterization of leukemias and lymphomas; B and T cell subset analysis for immunodeficiencies; stem cell counts; solid tumor DNA content and cell cycle analysis.
 
Cytogenetics
Every year, the cytogenetics laboratory processes a heavy volume of neoplastic specimens, utilizing both conventional cytogenetics as well as fluorescence in situ hybridization (FISH). FISH has become the recognized gold standard in determining the likely course of disease and appropriate treatment in leukemias such as chronic lymphocytic.
 
Molecular Biology
Our molecular biology laboratory performs Southern blot hybridization, polymerase chain reaction, and in situ hybridization studies for B and T cell gene rearrangements, many different chromosomal translocations for lymphoma and leukemia, including t(14;18), t(9;22), t(15;17), and Epstein-Barr virus. Many of the molecular tests can be performed on paraffin-embedded tissue.
 
Laboratory accreditation, certification and licensing
The Consultation Services Laboratories at City of Hope are fully accredited by the College of American Pathologists (CAP #22900-01), certified under the Clinical Laboratory Improvements Amendments of 1988 (CLIA '88, ID No. 05D0665695) and licensed by the state of California (CLF 268).

The laboratories participate in the CAP's external proficiency survey program for flow cytometry, immunophenotyping, immunohistochemistry, cytogenetics and molecular oncology. Specific details on our survey performance are available upon request.
 

Antibody Library

Antibody Library

Actin
AFP (alpha-fetprotein)
ALK protein
bcl-1
bcl-2
bcl-6
BerEp4
BG8
Calcitonin
Calretinin
CD1
CD2
CD3
CD4
CD5
CD7
CD8
CD10
CD15
CD16
CD20
CD21
CD23
CD30
CD31
CD34
CD35
CD343
CD45RA
CD45/45RB
CD45RO
CD56
CD57
CD68
CD79A
CD99
CD117 (c-kit)
CD138
CEA-Gold1
CEA-Gold5
c-erb-2
Chromogranin
Collagen-IV
DBA.44
Desmin
EBV-LMP
EMA (epithelial membrane  antigen)
ER (estrogen receptor)
Factor XIII (vWF)
FASCIN
GCDFP-15
GFAP
Granzyme B
HBME-1
HCG
Helico
Hemoglobin A
HLA-DR
HMB45
Inhibin
Kappa
Keratin 5/6
Keratin 8/18
Keratin 7
Keratin 8
Keratin 14
Keratin 19 (AE-1)
Keratin 20
Keratin cocktail (pan keratin)
34 βE 12 (HMW Keratin)
Ki-67
Lambda
Lysozyme
Melan-A
mes-13
MOC
MPX (myeloperoxidase)
NF (neurofilament)
p53
PacP (prostate acid phosphatase)
Perforin
PLAP (placential alkaline phosphatase)
PR (progesterone receptor)
Prolactin
PSA (prostate specifice antigen)
S100
SMA (smooth muscle actin)
Surfactant
Synaptophysin
Tdt
TGB (thyroglobin)
Thrombomodulin
TIA-1
TRAP
Tryptase
TTF-1
Villin
Vimentin
vWF (factor VIII)

Specimen Draw and Shipping Requirements

Specimen Draw and Shipping Requirements

Cytogenetics
Results for STATs, pre-BMT's and newly diagnosed leukemias are available within seven days. All others are available in seven to 10 days but our goal is to report results as soon as they are available. Complex studies may require additional days. Specimens are accepted Monday through Friday, 8 a.m. to 5:30 p.m.
 
Drawing specifications
  • Leukemia and myelodysplastic syndromes: A bone marrow aspirate is the specimen of choice in most cases of leukemia, myelodysplastic and myeloproliferative disorders.
  • Leukemia cytogenetics: Obtain 1 to 2 ml of bone marrow (minimum 0.5 ml) in a heparinized syringe (approximately 500 USP sodium heparin per ml of specimen) and transfer the specimen into a green top sodium heparin tube. Keep at room temperature and ship as soon as possible.
  • Leukemic peripheral blood: Draw 5 to 20 ml of peripheral blood into a green top sodium heparin tube. Mix well by inverting. Keep at room temperature and ship as soon as possible.
  • Lymphoma and solid tumor cytogenetics: Bone marrow core biopsies should be placed in HBSS or RPMI medium.
 
Flow cytometry / molecular diagnostics
The laboratory is open Monday through Friday, 8 a.m. to 5 p.m.
 
Drawing specifications
  • Peripheral blood: For leukemia and lymphoma panels, draw six green top tubes (sodium herapin) and one purple top tube. Maintain at room temperature and ship as soon as possible. ACD tubes are acceptable. Sodium lithium should not be used.
  • Bone marrow: Draw at least one purple top tube, maintain at room temperature and ship as soon as possible. ACD and sodium heparin tubes are acceptable.
 
 

Pathology Team

Pathology Team

Support This Program

Support this program

It takes the help of a lot of caring people to make hope a reality for our patients.  City of Hope was founded by individuals’ philanthropic efforts 100 years ago. Their efforts — and those of our supporters today — have built the foundation for the care we provide and the research we conduct. It enables City of Hope to strive for new breakthroughs and better therapies, ultimately helping more people enjoy longer, better lives.

For more information on supporting this specific program, please contact our Philanthropy Department at 800-667-5310 or philanthropy@coh.org. Or, to make a gift that supports all the research at City of Hope, donate online now.
 
We thank you for your support.
 
 
 
Quick Links
With Cancer, Expertise Matters

 
Cancer patients need to have confidence in their treatment plans by exploring all possible options. Often that means they should get a second opinion. For these four patients, getting a second opinion from experts at City of Hope was life-saving.
Supportive Care Calendar
 
With MyCityofHope your health information is right at your fingertips, anywhere, any time.


NEWS & UPDATES
  • Patients undergoing treatment at City of Hope know they will be receiving the best medical care available, that their treatment will be delivered with compassion and that their care will extend to their families. “When we treat a patient here, we treat a family,” says Jo Ann S. Namm, child life manager and spec...
  • Did you know that colorectal cancer equally affects men and women? Or that it’s the third-leading cause of cancer death in the U.S.? Most important, did you know that colorectal cancer is very treatable and highly curable if detected early? If you didn’t know these facts, it’s time to learn. M...
  • To celebrate the beginning of Lunar New  Year 2015, City of Hope honored not just a new lunar calendar, but also the diversity of the community it serves. On Jan. 21, as tens of thousands of people celebrated Lunar New Year (and the arrival of the Year of the Ram) in the streets of L.A.’s Chinatown, City of [&#...
  • The breakthroughs that have revolutionized cancer treatment, transforming cancer in many cases to a very manageable and even curable disease, started out as just ideas. “I will often tell patients there’s no therapy we’re using to help them that wasn’t derived from somebody’s idea in some laboratory, working la...
  • The prostate cancer screening debate, at least as it relates to regular assessment of prostate specific antigen levels, is far from over. The U.S. Preventive Services Task Force recommended against routine PSA screening for prostate cancer in 2012, maintaining that the routine use of the PSA blood test does mor...
  • Cancer patients should get more than medical treatment. They should get comprehensive, evidence-based care that addresses their full range of needs. That kind of patient-focused care is City of Hope’s specialty. Under the guidance of Dawn Gross, M.D., Ph.D., the new Arthur M. Coppola Family Chair in Suppo...
  • Think twice before tossing out those hormone replacement pills. Although a new Lancet study suggests that hormone replacement therapy could increase a woman’s risk of ovarian cancer, a City of Hope expert urges women to keep this news in perspective. Hormone replacement therapy is prescribed to help allev...
  • Don’t know what to take, or send, that friend of yours in the hospital? Try a paper plate — filled not with cookies or sweets, but an image of yourself. Ilana Massi, currently undergoing treatment at City of Hope for acute myeloid leukemia, can vouch for the power of such a gift. She’s surrounded herself [̷...
  • With precision medicine now a national priority, City of Hope has joined a novel research partnership designed to further understanding of cancer at the molecular level, ultimately leading to more targeted cancer treatments. The Oncology Research Information Exchange Network, or ORIEN, is the world’s larg...
  • The spinal cord is an integral part of the human body, connecting the brain to everything else. So when a tumor grows on the spine, any messages that the brain tries to send to the rest of the body are interrupted, making everyday tasks — such as walking — more difficult. This year an estimated 22,850 […]
  • Each year, thousands of patients with hematologic malignancies undergo allogeneic stem cell transplantation (that is, they receive a donor’s stem cells), offering them a chance at cure. Graft-versus-host disease is a potentially deadly complication of this therapy and occurs in approximately 25 to 60 perc...
  • Bertram Yuh, M.D., assistant clinical professor in the Division of Urology and Urologic Oncology at City of Hope, offers his perspective on the benefits of surgery for aggressive prostate cancer. For men walking out of the doctor’s office after a diagnosis of cancer, the reality can hit like a ton of bricks. Th...
  • Although many Hispanic women face a high risk of mutations in the BRCA1 and BRCA2 genes – increasing their risk of breast and ovarian cancer – screenings for these mutations can be prohibitively expensive in Mexico and other Latin American countries. As a result, too many women don’t get the information t...
  • Providing lung cancer treatments to patients when their cancer is at its earliest and most treatable stages will now be a more attainable goal: Medicare has agreed to cover lung cancer screening for those beneficiaries who meet the requirements. The only proven way to detect lung cancer early enough to save liv...
  • At City of Hope, innovative scientific research, important clinical studies and vital construction projects are all powered by philanthropy. Generous supporters fuel a powerful and diverse range of progress in science and medicine, enabling researchers and clinicians to improve cancer treatments and create cure...