A National Cancer Institute-designated Comprehensive Cancer Center

Make an appointment: 800-826-HOPE
Cytogenetics Core Laboratory Bookmark and Share

Cytogenetics Core Laboratory

City of Hope’s Cytogenetics Core Laboratory, supported by the National Cancer Institute-funded Cancer Center Support Grant, provides classic and molecular cytogenetics [fluorescence in-situ hybridization (FISH)] services to Beckman Research Institute researchers, as well as non-City of Hope investigators. FISH testing has proved invaluable as a diagnostic tool in many types of malignancies and is useful in determining both prognosis and course of treatment. The equipment in the Cytogenetics Core Laboratory is available to researchers on an appointment basis. The laboratory also provides training and consultation services in molecular cytogenetic testing.

Services and Equipment

Cytogenetics Core services available include:
  • Cell line characterization and tumor bank storage
  • Solid tumor cytogenetic analysis
  • FISH analysis /FISH enumeration only
  • Genotype-phenotype correlation via immunocytochemistry/FISH analysis
  • Gene mapping
  • Photomicrography Human 24-color karyotyping (SKY)
  • Mouse 20-color karyotyping (SKY)
  • HUMARA clonality assay
  • Human 19K BAC array analysis (DNA to data)
  • Consultation and training services
 
 
Probes Utilized
The Cytogenetics Core Laboratory has experience with all commercially-available probes (e.g., chromosome enumeration probes, painting probes, single copy or locus-specific probes, translocation probes, human and mouse SKY probes, etc.) and nick-translated DNA fragments provided by researchers as “homebrew” probes (nick translocation labeling may be performed by Cytogenetics Core lab personnel). The DNA fragments may be genomic DNA, cDNA or vector DNA; however, due to sensitivity limitations of our current instrumentation, unique sequence probes larger than 2.5 kb are required for mapping studies. If the probe is known to be amplified, probes larger than 1 kb may be used.
 

Equipment
The Cytogenetics Core Laboratory is equipped with high-resolution fluorescent and light photomicroscopes, and three computerized imaging systems (including the SKY Applied Spectral Imaging System and the Bioview System), which are able to capture, process and print microscopic images. Tissue culture facilities and equipment for probe labeling and hybridization are also available.

 

Fluorochromes commonly used with our fluorescent microscopes:
The fluorescent microscopes in the Cytogenetics Core are optimized for observation of the fluorochromes listed below. However, fluorochromes with similar excitation and emission wavelengths as those listed below may also be observed with our systems.

 

 

Fluorochrome                                                   Emission (nm)
FITC or Fluorescein (green)                           520
Texas-red (red)                                                  620
Rhodamine (red)                                              590
DAPI (blue)                                                         452
Spectrum green                                                538
Spectrum red                                                     612
Spectrum orange                                              588
Aqua                                                                    480
 
Applied Spectral Imaging SKY System

 
Bioview System
 
The Bioview Duet capture screen displaying a live fluorescent image (left) with its corresponding morphological image (right).  The lower right images provide a gallery view of all captured cells on this slide.  
 

Abstract for Grants

The City of Hope Cytogenetics Core laboratory provides classic and molecular cytogenetics [fluorescence in-situ hybridization (FISH)] services to the City of Hope and Beckman Research Institute scientific investigators. The Cytogenetics Core laboratory equipment, including high-quality fluorescence and light photomicroscopes, three computerized imaging systems, tissue culture facilities, and FISH instrumentation for probe labeling and hybridization, is available to City of Hope and Beckman Research Institute researchers on an appointment basis. The core laboratory also provides training, cell line tumor banking, and consultation services for applied molecular cytogenetic testing.

Contact Us

Joyce Murata Collins, Ph.D.
Director
626-256-HOPE (4673), ext. 62313
jcollins@coh.org
 
Location
City of Hope
1500 East Duarte Road
Duarte, CA 91010-3000

Northwest Building
Room 2265

Phone: 626-256-HOPE (4673), ext. 62025
Fax: 626-301-8877
 

Pricing

Current service offering and pricing can be found on our iLab Solutions site. Please contact us with any questions. 

 

 

Using the Facility

New users of the fluorescent microscope and imaging systems must schedule an introductory training session with Vicki Bedell at 626-256-HOPE (4673), ext. 62025, before using the system. Once trained, researchers may use the system independently.

In order to reserve the facility, City of Hope researchers may schedule microscope use via the online calendaring system in MS Outlook or contact the core facility.Non-City of Hope personnel should contact the core facility. All users should book the facility as far in advance as possible, and must complete and submit the Core Lab Services Request Form . If you have limited needs (e.g., a few photographs), you may request that these be processed by Cytogenetics Core facility personnel.
 
Supplies Needed
Most of the reagents needed are provided by the Cytogenetics Core Laboratory. However, researchers should specify the tissue culture medium required for their cell lines, and any special reagent(s) for their project (e.g., ASI Mouse SKY Probe kit). Users must also complete and submit theCytogenetics Core Lab Service Request Form.
 
Turnaround Time
The turn-around time is highly project-dependent. Please contactJoyce Collins, Ph.D.to discuss the project for estimated turn-around-times.

Cytogenetics Core Laboratory

Cytogenetics Core Laboratory

City of Hope’s Cytogenetics Core Laboratory, supported by the National Cancer Institute-funded Cancer Center Support Grant, provides classic and molecular cytogenetics [fluorescence in-situ hybridization (FISH)] services to Beckman Research Institute researchers, as well as non-City of Hope investigators. FISH testing has proved invaluable as a diagnostic tool in many types of malignancies and is useful in determining both prognosis and course of treatment. The equipment in the Cytogenetics Core Laboratory is available to researchers on an appointment basis. The laboratory also provides training and consultation services in molecular cytogenetic testing.

Services

Services and Equipment

Cytogenetics Core services available include:
  • Cell line characterization and tumor bank storage
  • Solid tumor cytogenetic analysis
  • FISH analysis /FISH enumeration only
  • Genotype-phenotype correlation via immunocytochemistry/FISH analysis
  • Gene mapping
  • Photomicrography Human 24-color karyotyping (SKY)
  • Mouse 20-color karyotyping (SKY)
  • HUMARA clonality assay
  • Human 19K BAC array analysis (DNA to data)
  • Consultation and training services
 
 
Probes Utilized
The Cytogenetics Core Laboratory has experience with all commercially-available probes (e.g., chromosome enumeration probes, painting probes, single copy or locus-specific probes, translocation probes, human and mouse SKY probes, etc.) and nick-translated DNA fragments provided by researchers as “homebrew” probes (nick translocation labeling may be performed by Cytogenetics Core lab personnel). The DNA fragments may be genomic DNA, cDNA or vector DNA; however, due to sensitivity limitations of our current instrumentation, unique sequence probes larger than 2.5 kb are required for mapping studies. If the probe is known to be amplified, probes larger than 1 kb may be used.
 

Equipment
The Cytogenetics Core Laboratory is equipped with high-resolution fluorescent and light photomicroscopes, and three computerized imaging systems (including the SKY Applied Spectral Imaging System and the Bioview System), which are able to capture, process and print microscopic images. Tissue culture facilities and equipment for probe labeling and hybridization are also available.

 

Fluorochromes commonly used with our fluorescent microscopes:
The fluorescent microscopes in the Cytogenetics Core are optimized for observation of the fluorochromes listed below. However, fluorochromes with similar excitation and emission wavelengths as those listed below may also be observed with our systems.

 

 

Fluorochrome                                                   Emission (nm)
FITC or Fluorescein (green)                           520
Texas-red (red)                                                  620
Rhodamine (red)                                              590
DAPI (blue)                                                         452
Spectrum green                                                538
Spectrum red                                                     612
Spectrum orange                                              588
Aqua                                                                    480
 
Applied Spectral Imaging SKY System

 
Bioview System
 
The Bioview Duet capture screen displaying a live fluorescent image (left) with its corresponding morphological image (right).  The lower right images provide a gallery view of all captured cells on this slide.  
 

Abstract for Grants

Abstract for Grants

The City of Hope Cytogenetics Core laboratory provides classic and molecular cytogenetics [fluorescence in-situ hybridization (FISH)] services to the City of Hope and Beckman Research Institute scientific investigators. The Cytogenetics Core laboratory equipment, including high-quality fluorescence and light photomicroscopes, three computerized imaging systems, tissue culture facilities, and FISH instrumentation for probe labeling and hybridization, is available to City of Hope and Beckman Research Institute researchers on an appointment basis. The core laboratory also provides training, cell line tumor banking, and consultation services for applied molecular cytogenetic testing.

Contact Us

Contact Us

Joyce Murata Collins, Ph.D.
Director
626-256-HOPE (4673), ext. 62313
jcollins@coh.org
 
Location
City of Hope
1500 East Duarte Road
Duarte, CA 91010-3000

Northwest Building
Room 2265

Phone: 626-256-HOPE (4673), ext. 62025
Fax: 626-301-8877
 

Pricing

Pricing

Current service offering and pricing can be found on our iLab Solutions site. Please contact us with any questions. 

 

 

Using the Facility

Using the Facility

New users of the fluorescent microscope and imaging systems must schedule an introductory training session with Vicki Bedell at 626-256-HOPE (4673), ext. 62025, before using the system. Once trained, researchers may use the system independently.

In order to reserve the facility, City of Hope researchers may schedule microscope use via the online calendaring system in MS Outlook or contact the core facility.Non-City of Hope personnel should contact the core facility. All users should book the facility as far in advance as possible, and must complete and submit the Core Lab Services Request Form . If you have limited needs (e.g., a few photographs), you may request that these be processed by Cytogenetics Core facility personnel.
 
Supplies Needed
Most of the reagents needed are provided by the Cytogenetics Core Laboratory. However, researchers should specify the tissue culture medium required for their cell lines, and any special reagent(s) for their project (e.g., ASI Mouse SKY Probe kit). Users must also complete and submit theCytogenetics Core Lab Service Request Form.
 
Turnaround Time
The turn-around time is highly project-dependent. Please contactJoyce Collins, Ph.D.to discuss the project for estimated turn-around-times.
Research Shared Services

City of Hope embodies the spirit of scientific collaboration by sharing services and core facilities with colleagues here and around the world.
 

Recognized nationwide for its innovative biomedical research, City of Hope's Beckman Research Institute is home to some of the most tenacious and creative minds in science.
City of Hope is one of only 41 Comprehensive Cancer Centers in the country, the highest designation awarded by the National Cancer Institute to institutions that lead the way in cancer research, treatment, prevention and professional education.
Learn more about City of Hope's institutional distinctions, breakthrough innovations and collaborations.
Support Our Research
By giving to City of Hope, you support breakthrough discoveries in laboratory research that translate into lifesaving treatments for patients with cancer and other serious diseases.
 
 
 
 
Media Inquiries/Social Media
 
CONNECT WITH US
Facebook  Twitter  YouTube  Blog
 


NEWS & UPDATES
  • The physical side effects of cancer can damage anyone’s self-confidence, but especially that of women who, rightly or wrongly, are more likely to find their appearance (or their own perception of their appearance) directly connected to their ability to face the world with something resembling ap...
  • The promise of stem cell therapy has long been studied in laboratories. Now, as medicine enters an era in which this therapy will be increasingly available to patients, the nurses who help deliver it will be in the spotlight. City of Hope, which has launched its Alpha Clinic for Cell Therapy and Innovation (ACT...
  • Just because you can treat a condition, such as high cholesterol, at the end of life — well, that doesn’t mean you should. That’s the basic lesson of a study to be published March 30 in JAMA Internal Medicine. The ramifications go far beyond that. The research, in which City of Hope’s Betty Fe...
  • The understanding of the relationship between genetics and cancer risk continues to grow, with more genetic testing than ever before available to patients. However, the adage that a little knowledge is a dangerous thing is applicable: Without context for what a test result means, and without meaningful guidance...
  • Standard prostate biopsies haven’t changed significantly in the past 30 years – nor have the problems inherent with them. Regular biopsies have an expected error rate: Tumors may potentially be undersampled and, 30 percent of the time, men who undergo a radical prostatectomy are found to have more aggress...
  • In the field of cancer, patients have had surgery, chemotherapy and radiation therapy as options. Now, as City of Hope officially opens the Alpha Clinic for Cellular Therapy and Innovation, patients battling cancer and other life-threatening diseases have another option: stem-cell-based therapy. The Alpha Clini...
  • How does the environment affect our health? Specifically, how does it affect our risk of cancer? City of Hope physicians and researchers recently answered those questions in an Ask the Experts event in Corona, California, explaining the underlying facts about how the environment can affect our health. Moderator...
  • Nurses and other medical professionals have come to understand that it’s not enough just to fight disease. They also must provide pain relief, symptom control, and an unrelenting commitment to improve patients’ quality of life — especially at the end of life. Not too long ago, this was a relatively ...
  • “Tonight, I’m launching a new precision medicine initiative to bring us closer to curing diseases like cancer.” These were the words of President Barack Obama on Jan. 20, 2015, during his State of the Union address. So what is precision medicine, and how close are we to making it a reality for...
  • March is Colon Cancer Awareness Month. How sad, yet how serendipitous, that the co-creator of “The Simpsons” Sam Simon passed away in March after a four-year battle against colon cancer. What message can we all learn from his illness that can help us prevent and overcome colon cancer in our own lives? Colon can...
  • Misagh Karimi, M.D., assistant clinical professor, is a medical oncologist at one of City of Hope’s newest community practice locations, located in Corona in Riverside County. A recent community health report from Corona’s public health department stated that obesity rates for teens and adults in Riverside Coun...
  • In 1975, the median survival for patients with ovarian cancer was about 12 months. Today, the median survival is more than 5 years. Although researchers and clinicians are far from satisfied, the progress in ovarian cancer treatment is encouraging, said Robert Morgan, M.D., F.A.C.P., professor of medical oncolo...
  • Colorectal cancer may be one of the most common cancers in both men and women, but it’s also one of the most curable cancers. Today, because of effective screening tests and more advanced treatment options, there are more than 1 million survivors of colorectal cancer in the United States. Here, colorectal...
  • Breast cancer treatment can damage a woman’s ability to become pregnant, making the impact on fertility one of the key factors that many consider when choosing a therapy regimen. Now a study has found that breast cancer patients treated with a hormone-blocking drug in addition to chemotherapy were less li...
  • My colleagues in the clinic know I’ve got a soft spot. Last week, a patient of mine offered me a fantastic compliment. “You’re looking younger these days, Dr. Pal!” she said, offering me a big hug as she proceeded out of the clinic room. Lovely, I thought. The early morning workouts are paying off. She continue...