A National Cancer Institute-designated Comprehensive Cancer Center

Make an appointment: 800-826-HOPE
Analytical Pharmacology Bookmark and Share

Analytical Pharmacology Core Facility (APCF)

The Analytical Pharmacology Core facility (APCF) encourages and facilitates collaborative research between basic scientists and clinicians by providing a wide range of analytical services that benefit both groups. The APCF conducts pharmacokinetic and pharmacodynamic studies for both chemotherapy clinical trials and peer-reviewed preclinical studies.
 
The primary services of the APCF are:
 
  • Assay development and analysis (LS/MS/MS, GC/MS, HPLC, and AAS) of chemotherapeutic agents and related compounds.
  • Study design and expert analysis of pharmacokinetic, pharmacodynamic, and metabolic data.
 
The facility’s services are available to both City of Hope and external researchers.
 

Research reported in this publication included work performed in the Analytical Pharmacology Core supported by the National Cancer Institute of the National Institutes of Health under award number P30CA33572. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
 

 

Services and Equipment

Services
Services currently offered by the Analytical Pharmacology Core facility:
  • Initial consultation on research projects
  • Study design and protocol review
  • Development, implementation, and validation of analytical methods.
  • Sample analysis (LC/MS/MS, GC/MS, HPLC, and AAS).
  • Pharmacokinetic programming and data analysis
  • Sample acquisition, tracking, and storage. Sample acquisition, storage analysis, including HPLC, LC/MS/MS and GC/MS, and microbiological assay methodologies
  • Participation in collaborative writing

Equipment
Analytical capabilities cover a wide range of available methods including:
  • LC/MC/MS (Waters Quattro Ultima and Quattro Premier XE
  • GC/MS (2 Shimadzu QP-5000's
  • HPLC with UV/Vis, fluorescence, and electrochemical detection (Shimadzu and Thermo Separations)
  • AAS with graphite furnace (PerkinElmer AAnalyst 300

HPLC capability includes four complete systems, which are integrated directly to dedicated computers running EZChrom software for automated post-run analysis. Three of the HPLC systems include automated injection systems for more convenient analysis of a large number of samples.

Instrument control and data acquisition for the Micromass mass detector and the associated Agilent HPLC are coordinated through a MassLynx-NT workstation running MassLynx and QuanLynx software. The GC/MS instrumentation is composed of a Shimadzu Model QP-5000 EI gas chromatograph/mass spectrometer, interfaced directly to a dedicated computer running CLASS-5000 software.

In addition to the chromatographic instrumentation, a Perkin Elmer AAnalyst 300 flameless atomic absorption spectrometer (AA) is available for the determination of metals and metal-containing compounds such as cisplatin and its analogs. Sample analysis by AA utilizes an automated injection system, with data acquisition and analysis using WinLab software on a dedicated computer.

 

Using the Facility

Scheduling Equipment
To access the Analytical Pharmacology Core facility, investigators should contact Timothy Synold to start the process.

Abstract for Grants

The APCF is located in the Shapiro Building and the analytical equipment is in room 1042. Freezers for sample storage are also located in the Beckman Center Freezer Farm, Room 1012. An additional HPLC with a diode array detector that is used part time for core service activities is located in room 1002 of the Fox North Research Building. HPLC capability includes four complete HPLC systems consisting of seven solvent delivery modules (4 Shimadzu LC-10A's, 2 Shimadzu LC-10AD's, 1 SpectraSystem P4000). HPLC detection capabilities cover a wide range of currently available methods, including UV/Vis (Shimadzu SPD-10AV), fluorescence (Shimadzu RF-10A), electrochemical (ESA models 5100A and 5200A), and photodiode array (SpectraSystem UV6000LP) detection systems. The Shimadzu HPLC systems are integrated directly into one of two dedicated PC's running Shimadzu EZChrom software for automated post-run analysis. Likewise, the SpectraSystem system is integrated to a dedicated PC running Chromquest (OEM version of EZChrom software). Three of the four HPLC systems include automated injection systems (2 Shimadzu SIL-10A's, 1 SpectraSystem AS3000) for more convenient analysis of a large number of samples. A Perkin Elmer AAnalyst 300 AA is available for the determination of metals and metal containing compounds, such as cisplatin and its analogs. Sample analysis by AA utilizes an automated injection system, with data acquisition and analysis using WinLab software on a dedicated PC. The GC/MS instrumentation is composed of a Shimadzu Model QP-5000 EI gas chromatograph/mass spectrometer, interfaced directly to a dedicated PC running CLASS-5000 software. A Micromass Quattro Ultima API Triple Stage Quadrupole Mass Spectrometer System (LC/MS/MS) provides exquisite selectivity and sensitivity for analytes in complex biological matrices. Instrument control and data acquisition for the Micromass mass detector and the associated Agilent HPLC are coordinated through a MassLynx-NT Workstation running MassLynx and QuanLynx software. As a result of high demand for LC/MS/.MS services, a second triple quadrupole tandem mass spectrometer (Waters Premier XE) was recently obtained to increase our capabilities. The front-end on the Premier XE is a Waters Agility Ultra-high Pressure Liquid Chromatograph (UPLC) that allows us to maximize sensitivity and minimize run-times, thereby increasing throughput. Like the Quattro Ultima, instrument control and data analysis on the Premier XE is performed with MassLynx and QuanLynx software. In addition, MetaboLynx software was purchased to aid in metabolite identification.

Pricing

Current pricing can be found on our iLab site. Please contact us for further questions.
 

Analytical Pharmacology Team

Contact Us

Edward Newman, Ph.D.
Co-director
626-256-HOPE (4673)
enewman@coh.org
 
Timothy W. Synold, Pharm.D.
Co-director
626-256-HOPE (4673)
tsynold@coh.org
 
City of Hope and Beckman Research Institute
1500 East Duarte Road
Duarte, CA  91010-3000
 
Shapiro Building
Room 1042
Phone: 626-256-HOPE (4673), ext. 62110
Fax: 626-301-8898
 

Analytical Pharmacology

Analytical Pharmacology Core Facility (APCF)

The Analytical Pharmacology Core facility (APCF) encourages and facilitates collaborative research between basic scientists and clinicians by providing a wide range of analytical services that benefit both groups. The APCF conducts pharmacokinetic and pharmacodynamic studies for both chemotherapy clinical trials and peer-reviewed preclinical studies.
 
The primary services of the APCF are:
 
  • Assay development and analysis (LS/MS/MS, GC/MS, HPLC, and AAS) of chemotherapeutic agents and related compounds.
  • Study design and expert analysis of pharmacokinetic, pharmacodynamic, and metabolic data.
 
The facility’s services are available to both City of Hope and external researchers.
 

Research reported in this publication included work performed in the Analytical Pharmacology Core supported by the National Cancer Institute of the National Institutes of Health under award number P30CA33572. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
 

 

Services and Equipment

Services and Equipment

Services
Services currently offered by the Analytical Pharmacology Core facility:
  • Initial consultation on research projects
  • Study design and protocol review
  • Development, implementation, and validation of analytical methods.
  • Sample analysis (LC/MS/MS, GC/MS, HPLC, and AAS).
  • Pharmacokinetic programming and data analysis
  • Sample acquisition, tracking, and storage. Sample acquisition, storage analysis, including HPLC, LC/MS/MS and GC/MS, and microbiological assay methodologies
  • Participation in collaborative writing

Equipment
Analytical capabilities cover a wide range of available methods including:
  • LC/MC/MS (Waters Quattro Ultima and Quattro Premier XE
  • GC/MS (2 Shimadzu QP-5000's
  • HPLC with UV/Vis, fluorescence, and electrochemical detection (Shimadzu and Thermo Separations)
  • AAS with graphite furnace (PerkinElmer AAnalyst 300

HPLC capability includes four complete systems, which are integrated directly to dedicated computers running EZChrom software for automated post-run analysis. Three of the HPLC systems include automated injection systems for more convenient analysis of a large number of samples.

Instrument control and data acquisition for the Micromass mass detector and the associated Agilent HPLC are coordinated through a MassLynx-NT workstation running MassLynx and QuanLynx software. The GC/MS instrumentation is composed of a Shimadzu Model QP-5000 EI gas chromatograph/mass spectrometer, interfaced directly to a dedicated computer running CLASS-5000 software.

In addition to the chromatographic instrumentation, a Perkin Elmer AAnalyst 300 flameless atomic absorption spectrometer (AA) is available for the determination of metals and metal-containing compounds such as cisplatin and its analogs. Sample analysis by AA utilizes an automated injection system, with data acquisition and analysis using WinLab software on a dedicated computer.

 

Using the Facility

Using the Facility

Scheduling Equipment
To access the Analytical Pharmacology Core facility, investigators should contact Timothy Synold to start the process.

Abstract for Grants

Abstract for Grants

The APCF is located in the Shapiro Building and the analytical equipment is in room 1042. Freezers for sample storage are also located in the Beckman Center Freezer Farm, Room 1012. An additional HPLC with a diode array detector that is used part time for core service activities is located in room 1002 of the Fox North Research Building. HPLC capability includes four complete HPLC systems consisting of seven solvent delivery modules (4 Shimadzu LC-10A's, 2 Shimadzu LC-10AD's, 1 SpectraSystem P4000). HPLC detection capabilities cover a wide range of currently available methods, including UV/Vis (Shimadzu SPD-10AV), fluorescence (Shimadzu RF-10A), electrochemical (ESA models 5100A and 5200A), and photodiode array (SpectraSystem UV6000LP) detection systems. The Shimadzu HPLC systems are integrated directly into one of two dedicated PC's running Shimadzu EZChrom software for automated post-run analysis. Likewise, the SpectraSystem system is integrated to a dedicated PC running Chromquest (OEM version of EZChrom software). Three of the four HPLC systems include automated injection systems (2 Shimadzu SIL-10A's, 1 SpectraSystem AS3000) for more convenient analysis of a large number of samples. A Perkin Elmer AAnalyst 300 AA is available for the determination of metals and metal containing compounds, such as cisplatin and its analogs. Sample analysis by AA utilizes an automated injection system, with data acquisition and analysis using WinLab software on a dedicated PC. The GC/MS instrumentation is composed of a Shimadzu Model QP-5000 EI gas chromatograph/mass spectrometer, interfaced directly to a dedicated PC running CLASS-5000 software. A Micromass Quattro Ultima API Triple Stage Quadrupole Mass Spectrometer System (LC/MS/MS) provides exquisite selectivity and sensitivity for analytes in complex biological matrices. Instrument control and data acquisition for the Micromass mass detector and the associated Agilent HPLC are coordinated through a MassLynx-NT Workstation running MassLynx and QuanLynx software. As a result of high demand for LC/MS/.MS services, a second triple quadrupole tandem mass spectrometer (Waters Premier XE) was recently obtained to increase our capabilities. The front-end on the Premier XE is a Waters Agility Ultra-high Pressure Liquid Chromatograph (UPLC) that allows us to maximize sensitivity and minimize run-times, thereby increasing throughput. Like the Quattro Ultima, instrument control and data analysis on the Premier XE is performed with MassLynx and QuanLynx software. In addition, MetaboLynx software was purchased to aid in metabolite identification.

Pricing

Pricing

Current pricing can be found on our iLab site. Please contact us for further questions.
 

Analytical Pharmacology Team

Analytical Pharmacology Team

Contact Us

Contact Us

Edward Newman, Ph.D.
Co-director
626-256-HOPE (4673)
enewman@coh.org
 
Timothy W. Synold, Pharm.D.
Co-director
626-256-HOPE (4673)
tsynold@coh.org
 
City of Hope and Beckman Research Institute
1500 East Duarte Road
Duarte, CA  91010-3000
 
Shapiro Building
Room 1042
Phone: 626-256-HOPE (4673), ext. 62110
Fax: 626-301-8898
 
Research Shared Services

City of Hope embodies the spirit of scientific collaboration by sharing services and core facilities with colleagues here and around the world.
 

Recognized nationwide for its innovative biomedical research, City of Hope's Beckman Research Institute is home to some of the most tenacious and creative minds in science.
City of Hope is one of only 41 Comprehensive Cancer Centers in the country, the highest designation awarded by the National Cancer Institute to institutions that lead the way in cancer research, treatment, prevention and professional education.
Learn more about City of Hope's institutional distinctions, breakthrough innovations and collaborations.
Support Our Research
By giving to City of Hope, you support breakthrough discoveries in laboratory research that translate into lifesaving treatments for patients with cancer and other serious diseases.
 
 
 
 
Media Inquiries/Social Media
 
CONNECT WITH US
Facebook  Twitter  YouTube  Blog
 


NEWS & UPDATES
  • Henry Ford said it well: “Working together is success.” For biomedical researchers, this is especially true. The challenges they face often require expertise from multiple fields to find answers and solutions. Scientists seeking cures for type 1 diabetes in particular must overcome biological, medical and techn...
  • Superheroes are making plenty of headlines as the summer blockbuster season opens. At City of Hope, a 9-year-old girl wept as she hugged her own superhero: someone who had the superpower of healing her cancer. He didn’t wear flashy armor or a cape, but rather a plaid shirt. He doesn’t have a secret ...
  • Known for his ability to bring together, and lead, effective research teams, world-renowned translational research scientist and physician Larry W. Kwak, M.D., Ph.D., has joined City of Hope in a key leadership role within the institution’s new Hematologic Malignancies and Stem Cell Transplantation Instit...
  • To detect melanoma, the most deadly form of skin cancer, at its earliest, most treatable stage, conduct a head-to-toe skin self-examination once a month to check for suspicious moles.   Unusual, or atypical, moles can ultimately develop into skin cancer. Here is the ABCDE guide to potentially cancerous mol...
  • “Superheroes,” “grateful” and “lifesavers”: All are words patients have used to describe their bone marrow donors. For donors, “a great feeling” and “the right thing to do” seems to sum up their view of donating the stem cells used to save someone’s life. Bone marrow transplants of...
  • Updated: May 1, 2015 More than a decade after joining the bone marrow registry during a blood drive at the United States Military Academy at West Point, Phil Ratcliff received a call that he was a match for a leukemia patient. By then, he’d left his military career to start his own financial business, married [...
  • Updated: May 1. For Lars Nijland, the reason to become a member of a bone marrow registry was simple. “I always thought there would be no easier way to save somebody’s life,” said the 24-year-old student at Germany’s University of Goettingen, who signed up for the registry during a drive on his campus. Ni...
  • Updated: May 1 No parent ever wants to see their child hurting or sick in any way. Joanne Cooper’s daughter Amanda wasn’t sick, though. She seemed healthy. Vibrant. A straight-A student whose only major health ailment had been bouts of stress-related nausea. Then a blood test revealed that Amanda – now 9 years ...
  • Noe Chavez became animated when he recalled the story: “We were running a health event, screening folks for diabetes,” said the enthusiastic City of Hope population health researcher, “and this man comes over and starts talking to us about the trouble he’s having with his eyes. I spoke with him, listened ...
  • When Keith McKinny, 29, was first diagnosed with lymphoma and leukemia in 2010, the first person he thought of was former boyfriend Jason Mullins. The two hadn’t been in contact with each other for some time, but McKinny couldn’t think of anyone else with whom he wanted to be during that difficult period....
  • Updated: May 1 Yesenia Portillo’s search for a bone marrow donor started close to home. Her brother, sister and seven cousins all underwent testing, but none of them were a close enough match to donate the bone marrow stem cells she desperately needed for her transplant. Yesenia, now almost 16, had always been ...
  • Some of City of Hope’s most high-impact achievements have arisen from City of Hope’s globally recognized bone marrow transplant (BMT) program. The annual Karl G. Blume – Gerhard Schmidt Memorial Lecture in Transplantation Biology & Medicine — commemorating two of the most influential and revered...
  • Guido Marcucci, M.D., wants to put himself out of business. A respected clinician and esteemed basic and translational scientist, Marcucci joins City of Hope as co-director of the Gehr Family Center for Leukemia Research within the Hematologic Malignancies and Stem Cell Transplantation Institute. In this positi...
  • To say that myelofibrosis patients need more treatment options would be an understatement. The severely low platelet counts, known as thrombocytopenia, that are one of the hallmark symptoms of the disease can lead to chronic fatigue and weakness that not only damage quality of life but, ultimately, shorten life...
  • Patients with metastatic colorectal cancer often stop responding to the primary drugs used against the disease, leaving them with few options and little hope. Determined to increase those options, doctors and researchers at City of Hope are conducting two clinical trials that could lead to new treatments for pe...