Cells make and interconvert these coenzymes in order to allow us to keep the electrons flowing. NAD coenzymes are at the essential core of all vital processes.
Though one could consider NAD+, NADH, NADP+ and NADPH the crown jewels of metabolism, they are unlike crown jewels that are locked inside a vault inside a castle patrolled by armed guards. We discovered that NAD coenzymes are exposed to the elements of metabolic stress.
Dozens of conditions of metabolic stress — including overnutrition, alcoholism, DNA damage, free radical damage, heart failure, central and peripheral neurodegeneration, postpartum, and coronavirus infection — disturb the NAD system, thereby challenging a tissue’s ability to self-repair, conduct fundamental bioenergetic processes, and convert biosynthetic inputs into working structures.
Our approach to NAD metabolism was initially based on enzyme characterization and gene discovery, particularly of the eukaryotic nicotinamide riboside (NR) kinase pathway to NAD, and the identification of NR and nicotinic acid riboside as NAD precursors. Since then, we developed quantitative targeted NAD metabolomic technologies to identify and characterize conditions of metabolic stress that disturb the NAD system.
We have repeatedly identified pathological and developmental conditions in which:
NAD+ and/or NADPH are under attack
Low abundance, stress-signaling metabolites such as NMN, cADPR or NAADP accumulate; and/or
The NAD gene set is altered at the transcriptional level
Significantly, we have found that bioenergetic crises, such as in heart failure and neurodegeneration, involve a simultaneous attack on NAD+ and induction of the NMRK1 and/or NMRK2 genes. This is evidence of an endogenous system that releases NR for the benefit of damaged cells. Moreover, in these conditions, we have found that the oral provision of NR repletes the NAD metabolome and results in substantial protection from the underlying insult.
We have also identified malignancies with specific disturbances in NAD metabolism that make them vulnerable to biomarker-informed targeting of particular NAD pathways. The lab is expanding. Please read our publications and stay in touch.
Principal Investigator: Charles Brenner, Ph.D.
The Alfred E. Mann Family Foundation Chair in the Department of Diabetes & Cancer Metabolism, Charles Brenner conducts research that focuses on NAD metabolomics and systems biology, coronavirus biology, postpartum, lactation and neurodevelopment, diabesity and fatty liver and genotype-specific cancer targeting.
Aging-Related Inflammation Driven By Cellular Senescence Enhances NAD Consumption Via Activation Of CD38+ Macrophages
Covarrubias, A.J., Lopez -Dominguez, J. A., Perrone, R., Kale, A., Newman, J., Iyer, S.S., Schmidt, M.S., Kasler, H.G., K.-O. Shin, Lee, Y.-M., Ben-Sahra, I., Ott, M., Brenner, C., Campisi, J., & Verdin E.
Mechanisms to reduce the cytotoxicity of pharmacological nicotinamide concentrations in the pathogenic fungus Candida albicans.(2021). The FEBS journal, 288(11), 3478–3506.
Ghugari, R., Tsao, S., Schmidt, M., Bonneil, E., Brenner, C., & Verreault, A.
IL8 drives an elaborate signal transduction process for CD38 to produce NAADP from NAAD and NADP+ in endolysosomes to effect cell migration. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(9), 1256
Nam, T.S., Park, D.R., Rah, S.-Y., Woo, T.G., Chung, H.T., Brenner, C., & Kim, U.-H.
Targeting NAD+ In Translational Research To Relieve Diseases And Conditions Of Metabolic Stress And Ageing. Mechanisms of ageing and development, 186, 111208. (2020)
Gilmour, B.C., Gudmundsrud, R., Frank, J., Hov, A., Hindkjær Lautrup, S., Aman, Y., Røsjø, H., Brenner, C., Ziegler, M., Tysnes, O.B., Tzoulis, C., Omland, T., Søraas, A., Holmøy, T., Bergersen, L.H., Storm-Mathisen, J., Nilsen, H., Evandro, F., & Fang, E.F.