The Analytical Pharmacology Core Facility (APCF) encourages and facilitates collaborative research between basic scientists and clinicians by providing extensive pharmacology expertise and a wide range of analytical services. To this end, the APCF conducts pharmacokinetic and pharmacodynamic studies for both clinical trials and peer-reviewed preclinical studies. The facility and its services are available to both City of Hope and non-City of Hope researchers, and it has been supported by City of Hope’s NCI-funded Cancer Center Support Grant since its establishment in 1994.
Key instrumentation includes 4 triple quadrupole mass spectrometers, including a Micromass Quattro Ultima, a Waters Quattro Premier XE, an AB Sciex QTRAP 5500, and a Waters Xevo-TQXS. All systems provide state-of-the-art selectivity and sensitivity for detecting and quantifying analytes in complex biological matrices and are interfaced with a range of HPLC front-end components. Additional key core equipment includes an Agilent 8800 inductively-coupled plasma triple quadrupole mass spectrometer (ICP-MS) for quantitation of metals and metal-containing compounds. For analysis of biological agents, the Core has two FLEXMAP 3D Bioplex instruments for multiplex immunoassays, an Isolight benchtop single cell cytokine analyzer, a Bio-Rad QX200 Droplet Digital PCR, two Bio-Rad CFX96 QPCR instruments, a FLUOstar OPTIMA multi-label plate reader, a Miltenyi Biotech MACSQuant Analyzer 10 flow cytometer, and a Guava PCA-96 analytical cell analyzer.